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Assessment of genetic diversity of iranian population of Anethum graveolens L. (dill) using AFLP molecular markers

Hariri Akbari F., Omidi M., Torabi S., Khoshkharam M., Bovard R., Shafiee M., Safakish kashani MB., Saeed P., Behjat Sasan, B.

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J. Bio. Env. Sci.4(6), 141-152, June 2014

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Abstract

Anethum graveolens L. (dill) is an important medicinal and industrial plant. The essential oil of this species is used in different industrial such as pharmacy, nutrition, perfume manufacturing and veterinary. The populations collected from different regions of the country were cultivated in order to investigate the genetic diversity of dill and were studied in aspect of genetic variation and its correlation with geographical distribution. 337 alleles were observed polymorphic and 108 alleles in monomorphic of total 455 scorable alleles. Percentage of polymorphic bands was equal to 74 and the number of alleles observed for each primer combination ranged from 18 to 60. The highest number of alleles was associated with M22-E2 and E11-M17 primer and the lowest value was observed in E2-M35 primer combination. The coefficient of genetic similarity was varied between genotypes from 56/0 to 88/0. The minimum of genetic similarity was between populations of Shahrekord, Ahvaz and Yasuj with similarity coefficient 34%. The maximum of similarity was observed among samples of 17 and 18 that genetic difference was very little and almost zero between them. Cluster analysis of populations using UPGMA algorithm and SM indicated high genetic diversity among populations of dil and also no correlation between molecular diversity and geographic distribution. The existence of genetic diversity in these samples confirms that the phytochemical and morphological differences of the samples is not simply due to environmental impacts, but are controlled by genetic factors as well. The results of this study can be useful in the management of germplasm of dill.

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Assessment of genetic diversity of iranian population of Anethum graveolens L. (dill) using AFLP molecular markers

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