Assessment of genetic diversity of wheat (Triticum aestivum L.) using agro-morphological characters and microsatellite markers

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Research Paper 01/10/2016
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Assessment of genetic diversity of wheat (Triticum aestivum L.) using agro-morphological characters and microsatellite markers

Karima Kara, Najla Mezghani, Olfa Saddoud Debbabi, Maher Madini, Malika Rached-Kanouni, M’barek Ben Naceur
Int. J. Biosci.9( 4), 92-101, October 2016.
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Abstract

Genetic diversity of ten bread wheat (Triticum aestivum L.) varieties was evaluated at the DNA level using 16 wheat microsatellites molecular markers (Simple Sequence Repeat-SSR) and 6 agro-morphological characters. SSR bands were scored across all genotypes, for presence (1) or absence (0) and transformed into 0/1 binary matrix. A pair-wise similarity matrix was generated with the software NTSYS. The Polymorphism Information Content (PIC) ranged from 0.13 to 0.70 respectively for the primer WMC 24 and WMC 50 with an average of 0.48 and 0.49 per primer pair.  The similarity coefficient between cultivars ranged from 0.33 and 0.90 with an average of 0.63. Most of the genotypes showed a high degree of genetic similarity. The highest genetic distance value of 0.90 has been scored between Milan/S87230/babax and Angi-4. The lowest genetic distance value of 0.33 has been scored between Hammam1 and Attila2Pastor. Genetic similarity values between genotypes, calculated by the molecular derived data, were used to produce a dendrogram. The genotypes were clustered in four clear groups according to their origin, pedigree and in some cases to phenotypic characters similarities. The morpho-agronomical variability was analyzed using the Principal Component Analysis (PCA).The results demonstrate the utility of microsatellite markers for detecting polymorphism to estimate genetic diversity.

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Ali Y, Atta BM, Akhter J, Monneveux P, Lateef Z. 2008. Genetic variability, association and diversity studies in wheat (Triticum aestivum L.) germplasm. Pakistan Journal of Botanic 40 (5), 2087-2097. https://www.researchgate.net/publication/228754594

Araus JL, Slafer GA, Reynolds MP, Royo C. 2002. Plant breeding in Cereals: what should we breed for? Annual of Botanic 89, 925-940. http://www.ncbi.nlm.nih.gov/pubmed/12102518

Babay E, Chaabane R, Mzid-Abdmouleh R, Ben Naceur M. 2015. Diversity of tunisian bread wheat genotypes revealed by Morpho-agronomical and microsatellite markers. Bioscience Journal 31(3) , 701-708.

Bohn M, Friedrich UH, Melchinger AE. 1999.  Genetic similarities among winter wheat cultivars determined on the basis of RFLPs, AFLPs and SSRs and their use for predicting progeny variance. Crop Science 39, 228–237. https://experts.illinois.edu/en/publications/

Chao S, Zhang W, Dubcovsky J,  Sorrells M. 2007. Evaluation of genetic diversity and genome- wide linkage disequilibrium among U.S. wheat (Triticum aestivum L.) germplasm representing different market classes. Crop Science 47, 1018-1030.  http://naldc.nal.usdgovnaldcdownload.xhtml?id=14927

Chen HB, Martin JM, Lavin M, Talbert V. 1994. Genetic diversity in hard red spring wheat based on sequence tagged-site PCR markers. Crop Science 34, 1629-1632. https://dl.sciencesocieties.org/publications/cs/abstracts/34/…/CS0340061628.

Cox TS, Lookhart GL, Walker D, Harrell ELG, Albers LD, Rodgers MD. 1985. Genetic relationships among hard red winter wheat cultivars as evaluated by pedigree analysis and gliadin polyacrylamide-gel electrophoretic patterns. Crop Science 25, 1058-1063. https://dl.sciencesocieties.org/publications/cs/abstracts/25/6/CS0250061058.

Devos KM, Gale MD. 1992. The use of random amplified polymorphic DNA markers in wheat. Theoretical 84, 567-572. http://link.springer.com/article/10.1007/BF00224153.

Devos KM, Bryan GJ, Collins AJ, Gale MD. 1995. Application of two microsatellite sequences in wheat storage proteins as molecular markers. Theoretical and Applied Genetics 90, 247-252. http://link.springer.com/article/10.1007/BF00222209.

Gonzalez FG, Slafer GA, Miralles DJ. 2003. Grain and floret number in response to photoperiod during stem elongation in fully and slightly vernalized wheat. Field Crops Research 81, 17-27. https://www.researchgate.net/publication/229113881.

Hai L, Guo H, Wagner C, Xiao S, Friedt W. 2008. Genomic regions for yield and yield parameters in Chinese winter wheat (Triticum aestivum L.) genotypes tested under varying different wheat materials. Plant Science 175, 226-232. https://www.researchgate.net/publication223085426.

Khan AJ, Azam F, Ali A. 2010. Relationship of morphological traits and yield in recombinant inbread wheat lines grown under drought conditions. Pakistan Journal of Botanic 42(1), 259-267. http://www.pakbs.org/pjbot/PDFs/42(1)/PJB42(1)259.pdf

Leisova L. Kucera L, Dotlacil L. 2007. Microsatellites as tool to evaluated and characterize bread wheat core collection. Wheat Production in stressed Environments 9, 771-778. https://www.researchgate.net/…/225251479

Manjarrez-Sandoval P, Carter TE, Webb DM, Burton JW. 1997. RFLP genetic similarity estimates and coefficient of parentage as genetic variance predictors for soybean yield. Crop Science 37, 698-703. https://dl.sciencesocieties.org/publications/cs/pdfs/37/3/CS0370030698

Maydup M L, Antonietta M, Guiamet JJ Graciano C, Lopez JR, Tambussi EA. 2010. The contribution of ear photosynthesis to grain filling in bread wheat (Triticum aestivum ). Field Crop Research 119, 48-58. https://www.researchgate.net/publication/222512691

Nei M, Li WH. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences U.S.A 76, 5269-5273. http://www.pnas.org/content/76/10/5269

Peng JR, Richards DE, Hartley NM, Murphy GP, Devos KM, Flintham JE, Beales J, Fish LJ, Worland AJ, Pelica F. 1999. “Green revolution’’ genes encode mutant gibberellin response modulators. Nature 400, 256-261. http://www.ncbi.nlm.nih.gov/pubmed/10421366

Plaschke J, Gangland WHMS, Röder MS. 1995. Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theoretical and Applied Genetics 91, 1001-1007. https://www.researchgate.net/publication/258147818

Prasad M, Varshney RK, Roy JK, Balyan HS, Gupta PK. 2000. The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat. Theoretical and Applied Genetics 100, 584-592. http://link.springer.com/article/10.1007/s001220050077

Rajaram S. 2005. Role of conventional plant breeding and biotechnology in future wheat production. Turkish Journal of Agriculture and Forestry 29, 105-111. http://journals.tubitak.gov.tr/agriculture/issues/

Röder MS, Plaschke J, König SU, Börner A, Sorrells ME, Tanksley SD, Ganal MW. 1995. Abundance, variability and chromosomal location of microsatellites in wheat. Molecular Genetics and Genomics 246, 327–333. http://link.springer.com/article/10.1007/BF00288605

Rohlf  FJ. 1989. NTSYS-pc: numerical taxonomy and multivariate analysis system. Analysis system version 2.02, Exeter Software, New York, N.Y: Setauket. http://www.exetersoftware.com/downloads/ntsysguide21.pdf

Salem KFM, El-Zanaty AM, Esmail RM. 2008. Assessing wheat (Triticum aestivum L.) genetic diversity using morphological characters and microsatellite markers. World Journal of Agricultural Sciences 4 (5), 538-544, http://citeseerx.ist.psu.edu/viewdoc/

Schloetterer C, Amos B, Tautz D. 1991. Conservation of polymorphic simple sequence loci in cetacean species. Nature 354, 63-65. www.ncbi.nlm.nih.gov/pubmed/1944571

Sokal RR, Michener CD. 1958. A statistical method for evaluating systematic relationships. The University of Kansas science bulletin 38 (2), 1409–1438, http://www.citeulike.org/user/druvus/article/1327877

Tambussi  E A, Nogues S, Araus JL. 2005. Ear of durum wheat under water stress: water relations and photosynthetic metabolism. Planta 221, 446-458. http://link.springer.com/content/pdf/10.1007/s00425-004-1455-7.pdf

Toklu F, Baloch FS, Karakoy T, Ozkan H. 2015. Effects of different priming applications on seed germination and some agromorphological characteristics of bread wheat (Triticum aestivum L.). Turkish Journal of Agriculture and Forestry 39, 1005-1013. http://journals.tubitak.gov.tr/agriculture/issues/

Vollmann J, Grausgruber H, Stift G, Dryzhyruk V, Lelley T. 2005. Genetic diversity in camelina germplasm as revealed by seed quality characteristics and RAPD polymorphism. Plant Breeding 124, 446-453. http://onlinelibrary.wiley.com/doi/

Yao J, Ma H, Yang X, Yao GU, Zhou M. 2014. Inheritance of grain yield and it’s correlated with yield components in bread wheat (Triticum aestivum L.). African Journal of Biotechnology 13(12), 1379-1385. http://www.academicjournals.org/journal/AJB/