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Assessment of genetic variability of 36 Populations of Sainfoin (Onobrychis sativa) based on RAPD markers

Mohamad Rasouli, Ali Ashraf Jafari, Seyed Reza Tabaei-Aghdaei, Parvin Salehi Shanjani, Farokh Darvish

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Int. J. Biosci.3(10), 15-26, October 2013

DOI: http://dx.doi.org/10.12692/ijb/3.10.15-26

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Abstract

Random amplified polymorphic DNA (RAPD) markers have been used to characterize the genetic diversity of 36 Iranian populations of sainfoin (Onobrychis sativa Scop.). Five out of 20 tested RAPD primers produced 79 polymorphic bands and the average band produced for each primer was 15.8 polymorphic bands. The highest and lowest percentage of polymorphic loci was obtained for the population Divandara (80%) and the population Aligodarz3 (49%), respectively, with average 66%. Dendrograms were prepared using cluster analysis based on Nei’s distance coefficients. Clustering pattern, made on the basis of RAPD data, grouped the accessions differently and gave no clear indication of origin/source. Further examination of the different components of genetic variation by analysis of molecular variance indicated that larger proportions of variability existed within populations (78%). The results also showed that comprehensive germplasm collection in major geographic regions and exploitation of the existing variation are required to widen the genetic base and sample the full extent of the available variation in breeding strategies for sainfoin.

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Assessment of genetic variability of 36 Populations of Sainfoin (Onobrychis sativa) based on RAPD markers

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