Assessment of genetic variation among Rosa species using ISSR genetic markers
Paper Details
Assessment of genetic variation among Rosa species using ISSR genetic markers
Abstract
In this study, Inter-Simple Sequence Repeats (ISSR) markers were applied to assess genetic diversity and genetic relationships among seven species of Rosa. Six of ten used primers could amplify 81 scorable ISSR loci which 71 bands were polymorphic (86%). The 811 and 827 primers produced minimum and maximum bands, respectively. The percentage of polymorphic bands ranged from 60 to 100 with an average of 86.25 %. The size of amplified DNA fragments was ranging between 500 to 2500 bp which was used for calculation and statistical analysis. The cluster analysis based on the presence or absence of bands was performed by Jaccard’s similarity coefficient, based on Unweighted Pair Group Method with Arithmetic Averages (UPGMA). Similarity coefficient ranged from 0.30 to 0.66. Based on cluster analysis, the dendrogram revealed three main clusters. First cluster was divided into two subgroups. This investigation showed that genetic distance was relatively significant among these species. The results also propose that ISSR marker is a useful tool for evaluation of genetic diversity and relationships among different Rosa genotypes.
Agaoglu Y, Ergul A, Baydar N. 2000. Molecular analyses of genetic diversity of oil Rose (Rosa damascene Mill) grown in Isparta (Turkey) region. Biotechnology and Biotechnological Equipment 14, 16-18.
Baydar Ng, Baydar H, Debener T. 2004. Analysis of genetic relationships among Rosa damascena plant grown in Turkey by using AFLP and microsatellite markers. Journal of Biotechnology 111, 263-267.
Chowdhury MA, Vandenberg V, Warkentin T. 2002. Cultivar identification and genetic relationship among selected breeding lines and cultivars in chickpea (Cicer arietinum L.). Euphytica 127, 317– 325.
Darvishzade R, Azizi M, Hatamimaleki H, Bernousi I, Abdollahimandolkani B, JafariM, Sarrafi A. 2010. Molecular characterization and similarity relationships among sunflower (Helianthus annuus L.) inbred lines using some mapped simple sequence repeats. African Journal of Biotechnology 439, 7280-7288.
Debener T, Mattiesch L. 1998. Effective pairwise combination of long primers for RAPD analyses in Roses. Plant Breeding 117, 147-151.
Degenerate M, Mattiesch L. 1999. Construction of a genetic linkage map for roses using RAPD and AFLP markers. Theoretical and Applied Genetics 99, 891-899.
Dirlewanger E, Cosson P, Tavaud M, Aranzana MJ, Poizat C, Zanetto A, Arusplaigret F. 2002. Development of microsatellite markers in peach [Prunus persica (L.) Batsch] and their use in genetic diversity analysis in peach and sweet cherry (Prunus avium L). Theoretical and Applied Genetics 105, 127-138.
Ge XJ, Yu Y, Zhao NX, Chen HS, Qi WQ. 2003. Genetic variation in the endangered Inner Mongolia endemic shrub Tetraena mongolica Maxim (Zygophyllaceae).Biological Conservation 111, 427– 43.
Gudin S. 2000. Rose: genetics and breeding. Plant Breed 17, 59-189.
Gupta SH, Srivastava M, Mishra GP, Naik PK, Chauhan RS, Tiwari SK, Kumar M, Singh R. 2008. Analogy of ISSR and RAPD markers for comparative analysis of genetic diversity among different Jatropha curcas genotypes. African Journal of Biotechnology 42, 30-43.
Kaur N, Sharma RK, Sharma M, Singh V, Ahuja PS. 2007. Molecular Ealuation and Micropropagation of Field Selected Elites of R.damascena Gen. Plant physiology 33, 171-186.
Mahmood N, Piacente S, Pizza C, Burke A, Khan AI, Hay AJ. 1996. The Anti-HIV Activity and Mechanisms of Action of Pure Compounds Isolated from Rosa damascene .Biochemistry and Biophysics Research Communication 20, 73-79.
Millan T, OsunaF, Cobos S, Torres AM, Cubero JI. 1996. Using RAPDs to study phylogenetic relationships in Rosa. Theoretical and Applied Genetics 92, 273–277.
Paplauskiene V, Dabkeviciena G. 2008.Genetic variability determination using ISSR-PCR markers in red clover varieties. Biologica 54, 56-59.
Phillips R, Rix M. 1988. Roses. Random House, New York, 224 p.
Pezhmanmehr M, Hassani ME, Jahansooz F, Najafi AA, Sefidkon F, Mardi M, Pirsiedi M. 2009. Assessment of genetic diversity in some Iranian populations of Bunium persicum using RAPD and AFLP markers. Iranian journal of Biotechnoogy 33, 202-204.
Porter WM, Smith DH. 1982. Detection of identification errors in germplasm collection. Crop Scientia 22, 701–703.
SamieiL, Naderi R, Khalighi A, Shahnejatbushehri AA, Mozaffarian V, Esselink GD, Kazempourosaloo S, Smulders MJM. 2010. Genetic diversity and genetic similarities between Iranian rose. Journal of Horticultural Science & Biotechnology 85, 231–237.
Sarkhosh A, Zamani Z, Fatahi R, Ebadi A. 2006. RAPD markers reveal polymorphism among some Iranian pomegranate genotypes. Scientia Horticulturae 111, 24-29.
Shulaev V, Korban SS, Sosinski B, Abbott AG, Aldwinckle HS, Folta KM, Iezzoni A, Main D, Arus P, Dandekar AM, Lewers K, Brown SK, Davis TM, Gardiner SE, Potter Dand Veilleux RE. 2008. Multiple Models for Rosaceae Genomics. American Society of Plant Biologists 147, 985–1003.
Sofalian O, Chaparzadeh N, Javanmard A, Hejazi MS. 2008. Study the Genetic Diversity of Wheat Landraces from Northwest of Iran Based on ISSR Molecular Markers. Journal of Agriculture & Biology 10, 465–8.
Tabaeiaghdaei SR, Hosseinimonfared H, Fahimi H, Ebrahimzade H, Jebelly M, Naghavi MR, Babaei A. 2006. Genetic Variation Analysis of Different Population of Rosa damascena in NW.Iran using RAPD Markers. Iranian journal of Botany 12, 121-127.
Teng Y, Tanabe K, Tamaru F, Itai A. 2002. Genetic relationship of Pyrus species and cultivar native to East Asia revealed by randomly amplified polymorphic DNA markers. Journal of the American Society for Horticultural Science 127, 262-270.
Tripathyogendra K, Gurha P, Ghosh D, Kumar RV, Prakash V. 2007. Determination of Phytogenetic Relationships among Isoetes Species Using Random Primers. Turkish Juornal of Botany 31, 367-372.
Yao Q, Yang K, Pan G, Romg T. 2007. Genetic Diversity of Maize (Zea mays L.) Landraces from Southwest China Based on SSR Data . Journal of Genetics and Genomics 349, 851-860.
Yousefi B, Tabaei SR, Darvish F, Assareh MH. 2009. Flower yield performance and stability of Rosa damascene Mill.landraces under different ecological conditions. Scientia Horticulturae 121, 333-33.
Leila Mirzaei, Fatemeh Rahmani, Mina Beigmohamadi (2015), Assessment of genetic variation among Rosa species using ISSR genetic markers; JBES, V7, N3, September, P254-260
https://innspub.net/assessment-of-genetic-variation-among-rosa-species-using-issr-genetic-markers/
Copyright © 2015
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0