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Assessment of genetic variation among Rosa species using ISSR genetic markers

Leila Mirzaei, Fatemeh Rahmani, Mina Beigmohamadi

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J. Bio. Env. Sci.7(3), 254-260, September 2015

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Abstract

In this study, Inter-Simple Sequence Repeats (ISSR) markers were applied to assess genetic diversity and genetic relationships among seven species of Rosa. Six of ten used primers could amplify 81 scorable ISSR loci which 71 bands were polymorphic (86%). The 811 and 827 primers produced minimum and maximum bands, respectively. The percentage of polymorphic bands ranged from 60 to 100 with an average of 86.25 %. The size of amplified DNA fragments was ranging between 500 to 2500 bp which was used for calculation and statistical analysis. The cluster analysis based on the presence or absence of bands was performed by Jaccard’s similarity coefficient, based on Unweighted Pair Group Method with Arithmetic Averages (UPGMA). Similarity coefficient ranged from 0.30 to 0.66. Based on cluster analysis, the dendrogram revealed three main clusters. First cluster was divided into two subgroups. This investigation showed that genetic distance was relatively significant among these species. The results also propose that ISSR marker is a useful tool for evaluation of genetic diversity and relationships among different Rosa genotypes.

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Assessment of genetic variation among Rosa species using ISSR genetic markers

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