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Assessment of genotypic variation and self-incompatibility in cauliflower (Brassica oleracea var. botrytis) genotypes

Research Paper | February 1, 2020

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Muhammad Najeebullah, Saba Aleem, Etlas Amin, Rasheda Aslam, Mehvish Tahir, Rabia Kanwal, Nusrat Parveen

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Int. J. Biosci.16( 2), 173-182, February 2020

DOI: http://dx.doi.org/10.12692/ijb/16.2.173-182


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Cauliflower is an important cool season vegetable. Knowledge of genetic variation and self incompatibility of cauliflower genotypes may be important as it provides a base for breeding strategies. In this study, genetic variation and strength of self-incompatibility were assessed in different exotic and indigenous genotypes. Mean comparison of genotypes revealed significant variability in all the genotypes regarding quantitative traits i.e. curd weight, plant weight, biomass and curd yield. Extensive variability was also observed for qualitative traits with Shannon–Weaver diversity index (H’ = 0.60- 1.08) among the studied genotype. Coverage of curd by leaves, leaf attitude and curd shape in longitudinal section showed the highest degree of genetic variation. Genotypes also showed variation regarding vegetation period, in this respect HCF-12 was characterized as early maturing while HCF-23 as a late maturing genotype. Regarding self-incompatibility all the genotypes showed significant difference when assessed by artificial selfing method with coefficients of variation (CV= 40%) while less variations were observed through natural selfing method (CV= 2%). On the basis of artificial sellfing HCF-22 and HCF-23 (SI >95%) genotypes were categorized into strongly self-incompatible and HCF-12, HCF-13 into self-compatible (50%≤SI≥5%). Snow Wizard, Snow Mountain and FDIII were classified into partial self-incompatible (95%≤SI≥50%). These findings of genotypic variation in phenotypic traits and self-incompatibility expressed the usefulness of these genotypes as parents in the cauliflower breeding program.


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Assessment of genotypic variation and self-incompatibility in cauliflower (Brassica oleracea var. botrytis) genotypes

Amin E, Raza MM, Iqbal M, Najeebullah M. 2018. Kheera local: a new high yielding, well adaptable and heat tolerant cucumber (Cucumis sativus L.) Variety. Journal of Agricultural Research 56, 13-16.

FSC, RD. 2017, Federal seed certification and registration department, Islamabad, 2017-18.

Bateman AJ. 1955. Self‐incompatibility systems in angiosperms. III. Cruciferae. Heredity 9, 52–68.

Chittora A, Singh DK.  2015. Genetic variability studies in early cauliflower (Brassica oleracea var. Botrytis l.). Electronic Journal of Plant Breeding 6, 842-847.

Damake SR, Singh BP, Dubey RK, Ram HH. 2004. Level of self-incompatibility in tropical cauliflower (Brassica oleracea var. Botrytis L. Pantnagar Journal of Research 2, 65-68.

Gray, Crisp. 1977. Breeding system, taxonomy, and breeding strategy in cauliflower (Brassica oleracea var. Botrytis L.), Euphytica 26, 369-375.

Higdon JV, Delage B, Williams DE, Dashwood RH. 2007. Cruciferous vegetables and human cancer risk: epidemiologic evidence and mechanistic basis. Pharmacological Research 55, 224-236.

Jung HJ, Ahmed NU, Park JI, Chung MY, Cho YG, Nou IS. 2013. Molecular genetic aspects of self-incompatibility in Brassicaceae. Plant Breeding and Biotechnology 1, 205-217.

Kitashiba H, Nasrallah JB. 2014. Self-incompatibility in Brassicaceae crops: lessons for interspecific incompatibility. Breeding Science 64, 23-37.

Kumar V, Singh DK, Panchbhaiya A, Singh N. 2017. Genetic divergence studies in mid-season cauliflower (Brassica oleracea var. Botrytis L.) Through principal component analysis (PCA) and D2 analysis. International journal of chemical studies 5, 989-993.

Li X, Fang Z, Liu Y, Wang H, Shen D. 2008. Descriptions and data standard for cauliflower (Brassica oleracea L. Var. Botrytis) and Brassica oleracea L. Var. Italica plenck. China agriculture Press, Beijing, China

Magurran A. 2004. Measuring biological diversity. Oxford: Blackwell.

Nieuwhof M. 1974. The occurrence of self-incompatibility in cauliflower (Brassica oleracea var. Botrytis L. Subvar. Cauliflora dc.) and the possibilities to produce uniform varieties. Euphytica 23, 473-478.

Pilatova M, Chripkova M, Mojzis J. 2011. Cruciferous vegetables in cancer prevention. Acta Facultatis Pharmaceuticae Universitatis Comenianae, 58, 62-71.

Purugganan MD, Boyles AL, Suddith JI. 2000. Variation and selection at the cauliflower floral homeotic gene accompanying the evolution of domesticated brassica oleracea. Genetics 155, 855-862.

Rabara RC, Marilyn CF, Celia LD, Newingham CVM, Gabriel OR. 2014. Phenotypic diversity of farmers’ traditional rice varieties in the Philippines, Agronomy 4, 217-241.

Ruffio-Chable V, Herve Y, Dumas C, Gaude T. 1997. Distribution of s-haplotypes and its relationship with self-incompatibility in Brassica oleracea. Part 1. In inbred lines of cauliflower (B. oleracea var botrytis). Theoretical Applied Genetics 94, 338-346.

Sahu PK. 2017. Studies on genetic variability and self-incompatibility in indian cauliflower (Brassica oleracea (L.) Var. Botrytis). M. Sc. Thesis. Department of Horticulture, Indira Gandhi Agriculture University Raipur.

Sarwar B. 2008. Variety descriptor for cauliflower (Brassica oleracea var. Botrytis L.), Facilitation unit for participatory vegetable seed & nursery production program.

Shakhatreh Y, Haddad N, Alrababah M, Grando S, Ceccarelli S. 2010. Phenotypic diversity in wild barley (Hordeum Vulgare L. ssp. Spontaneum (C. Koch) Thell.) accessions collected in Jordan. Genetic Resources and Crop Evolution 57, 131–146.

Shannon CE, Weaver W. 1949. The mathematical theory of communication. Urbana: University of Illinois press.

Singh H, Singh V, Kumar R., Baranwal D, Ray P. 2014. Assessment of genetic diversity based on cluster and principal component analyses for yield and its contributing characters in bitter gourd. Indian Journal of  Horticulture 71, 55-60.

Singh S, Vidyasagar. 2012. Effect of common salt (NaCl) sprays to overcome the self-incompatibility in the S-allele lines of Brassica oleracea var. capitata L. SABRAO Journal of Breeding and Genetics 44,339-348.

Singh J, Singh M, Singh R. 2002. Level of self-incompatibility in tropical cauliflower (Brassica oleracea L.Var. Botrytis). Vegetable Science.

Tahir M, Aslam R, Saeed A. 2018. Determination of genetic diversity in onion genotypes using multivariate analysis under short day conditions. Journal of Agriculture Research 56, 169-172.

Vanlalneihi B, Saha P, Kalia P, Singh S, Saha N, Kundu A, Singh N, Bhowmik A. 2019. Genetic and principal component analysis for agro-morphological traits, bioactive compounds, antioxidant activity variation in breeding lines of early indian cauliflower and their suitability for breeding. Journal of Horticulture  Science and  Biotechnology, 1-13.

Watts L. 1963. Investigations into the breeding system of cauliflower Brassica oleracea var. Botrytis (L.). Euphytica 12, 323-340.

Zhu S, Zhang X, Liu Q, Luo T, Tang Z, Zhou Y. 2018. Phenotypic variation and diversity of cauliflower (Brassica oleracea var. Botrytis) inbred lines. International Jornal of Agriculture and Biology 20, 1041-1048.