Assessment of in vitro antitumor potential of luteolin loaded polymeric nanofromulations

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Research Paper 01/11/2018
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Assessment of in vitro antitumor potential of luteolin loaded polymeric nanofromulations

Umara Afzal, Muhammad Gulfraz, S.M. Saqlan Naqvi, Nadeem Akhtar Abbasi, Muhammad Awais, Warda Ahmad, Salma Batool
Int. J. Biosci.13( 5), 195-204, November 2018.
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Non-small cell lung cancer (NSCLC) is one of the fetal type of lung cancer worldwide with high resistant against current chemotherapeutic agents due to increase their aggressive features. Many plant derived natural compounds have been found to possess antimetastatic potential against NSCLC by inhibiting molecular product and correspondingly their growth. Luteolin is plant derived flavonoid with potential antioxidant, anti-inflammatory and anticancer potential against multiple malignancies but its bioavailability is low due to hydrophobic nature with less half-life. Current study was designed to increase bioavailability of luteolin by formulate in physiologically stable and biodegradable polymer Poly (lactic-co-glycolic) acid. Surface modified PLGA with PEG (PEG-PLGA NPs) was used as comparative formulation. Both formulations were made by single emulsion method. Physiochemical characteristics including surface morphology, size and charge and stability in NaCl and serum medium was done for both formulations. Invitro antitumor potential of free and formulated luteolin against Non-small cell lung cancer (NSCLC) was done using CCK-8 assay and % viability was determined. Results from physiochemical characterization showed 156nm particles of PEG-PLGA and slightly large 350nm sized particles of PLGA suspended in PBS whereas TEM images showed <100nm sized PEGylated NPs with high payload of luteolin drug with sustained release up to two days as determined by HPLC analysis. Invitro tumor growth inhibition assay showed cytotoxic potential of nanofromulation of luteolin as compared to free luteolin. Results of both PLGA and PEG-PLGA formulations of luteolin showed promising potential for further in vivo therapeutic approach against NSCLC.


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