Assessment of nickel nano particles induced spleenotoxicity in male sprague dawley rats

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Research Paper 01/01/2019
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Assessment of nickel nano particles induced spleenotoxicity in male sprague dawley rats

Mehwish Iftikhar, Muhammad Ali, Farhat Jabeen, Azhar Rasool, Muhammad Kashif Zahoor
Int. J. Biosci.14( 1), 131-139, January 2019.
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Nickel compounds are classified as carcinogens therefore; there is a need to determine the toxic effects of nickel nanoparticles (Ni-NPs) on organisms’ health. With enhancement in nanotechnology, Ni-NPs are widely used in various fields of daily life. In the present study, Ni-NPs were used to determine their accumulation and toxic effects on histological profiles of spleen of male Sprague Dawley rats. For this purpose twenty five male Sprague Dawley rats weighing (200-250g) were procured from the animal house of Government College University Faisalabad after approval of the ethical committee on animal experimentation of Government College University Faisalabad. Rats were divided into five groups (n=5) as control (without any treatment), saline (treated with 0.9% sodium chloride for the equivalency of shock) and three nano treated groups (i.e., Ni-NPs @ of either 15 or 30 or 45mg/kg b.wt) with five replicates in each group were used to determine the accumulation and toxic effects of Ni-NPs on histological profile of spleen. At the end of the experiment, histological observations showed abnormalities in spleen structure with the formation of macrophage and increased megakaryocytes number, blood vessel damage and alteration in splenic capsule thickness. Fibrosis and necrosis was also observed. All these histological changes along with bio-distribution of Ni in spleen were dose dependent. Histological alterations and accumulation was more adverse at high dose (45mg/kg b.wt) in comparison with low and medium dose as well as control. Therefore, it is concluded that at higher concentration, exposure to Ni NPs is hazardous and should be handled with care.


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