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Assessment of selected culture media for competent growth characteristics of Ceratocystis manginecans (Cause of Mango Sudden Death)

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Research Paper 01/05/2017
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Assessment of selected culture media for competent growth characteristics of Ceratocystis manginecans (Cause of Mango Sudden Death)

Kokab Jabeen, Shahzad Asad
J. Bio. Env. Sci.10( 5), 148-154, May 2017.
Certificate: JBES 2017 [Generate Certificate]
Key: Ceratoc ystismanginecansCulture mediaMango sudden death (MSD)

Abstract

Comprehensive and critical facts of aspects which influence patterns of growth and nutrition of the fungi is precondition for research related to the relationship of host and pathogen. A number of media compositions likewise influence the colony morphology of fungus. Most conjoint and distressing disease in mango trees is Mango sudden death (MSD) caused by Ceratocystis manginecans. Present study is aimed to evaluate the influence of selected six different culture media (agar and broth media) on sporulation; colony characteristics and growth of the C. manginecans isolated from MSD infected mango trees. Statistical analysis of the data gathered revealed that all the treatments were significantly different from each other. Among solid media, Malt Extract Agar and Carrot Juice Agar supported best growth of C. manginecans89.53mm and 85.31mm respectively. Among broth media Carrot Juice supported better growth (503.33mg dry weight) than other media. The Characteristics of growth like color of substrate, colony color and margins, mycelia topography as well as sporulation of the C. manginecans were studied. Best sporulation of C. manginecans was shown in Malt Extract Agar media. Selection of most effective culture media for efficient growth characteristics of C. manginecans is necessary for its physiological and taxonomical study as well as for suitable disease management strategies.

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Diba K, Kordbacheh P, Mirhendi SH, Rezaie S, Mahmoudi M. 2007. Identification of Aspergillus species using morphological characteristics. Pakistan Journal of Medical Sciences 23, 867-872.

Khuhro RD, Nizamani SM, Abbasi QD, Solangi GS, Jiskani MM. 2005. Mango Tree Mortality due to Asian ambrosia beetle, Xylosandrus crassiusculus Mot. (Coleoptera: Scolytidae) Pak. J. Agri. Agril. Engg., Vet. Sc 21(1), 39-42.

Koley S, Mahapatra SS. 2015. Evaluation of culture media for growth characteristics of Alternaria solani, causing early blight of tomato. Journal of Plant Pathology and Microbiology S1, 2-5. DOI: 10. 4172/2157-7471. 1000S1-005.

Masood A, Saeed S, Iqbal N, Malik MT, Kazmi MR. 2010. Methodology for the evaluation of symptoms severity of mango sudden death syndrome in Pakistan. Pakistan J. Bot. 42(2), 1289-1299.

Mishra PT, Mishra V. 2012. Effect of media, temperature and pH on growth of Alternaria alternata causing leaf spot of cotton. Annals of Plant Protection Sciences 20, 246-247.

Munde VG, Diwakar MP, Thombre BB, Dey U. 2013. Cultural and morphological characters of Alternaria solani on different media. Bioinfolet. 10, 984-986.

Rashid. A,Iram S, Ahmad I. 2014. Molecular characterization of Ceratocystis Manginecans sp. from Mango in Pakistan.  Pakistan Journal of Agricultural Sciences 51(3), 1-5.

Saha A, Mandal P, Dasgupta S, Saha D. 2008. Influence of culture media and environmental factors on mycelial growth and sporulation of Lasiodiplodia theobromae (Pat.) Griffon and Maubl. Journal of Environmental Biology 29, 407-410.

Wyk VM, Al Adawi AO, Khan IA, Deadman ML, Al Jahwari AA, Ploetz R, Wingfield BD, Al Subhi AM, Wingfield MJ. 2007. Ceratocystis manginecans sp. Nov., causal agent of a destructive mango wilts disease in Oman and Pakistan. Fungal Diversity 27, 213-230.

Zain ME, Razak AA, El-Sheikh HH, Soliman HG, Khalil AM. 2009. Influence of growth medium on diagnostic characters of Aspergillus’s and Penicillium species. African Journal of Microbiology Research 3(5), 280-286.

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