Welcome to International Network for Natural Sciences | INNSpub

Assessment of some polymers for the formulation of rhizobial liquid inoculants

Research Paper | November 1, 2017

| Download 6

Mohammed A. Hassan, Somaya S. Mohamed, Migdam E. Abdelgani

Key Words:

Int. J. Biosci.11( 5), 277-285, November 2017

DOI: http://dx.doi.org/10.12692/ijb/11.5.277-285


IJB 2017 [Generate Certificate]


The present study was conducted to screen and evaluate four polymeric additives, polyvinylpyrrolidone (PVP), polyvinyl alcohol (PVA), polyethylene glycol (PEG) and Gum Arabic at different concentrations for their ability to maintain and support bacterial viability of rhizobial strains TAL 380, USDA 110 and local isolates ENRRI 12 and ENRRI 1 during the initial period of growth compared with growth in YEM medium without additives. The comparison of the different concentrations for each polymer was carried out after 8 days of incubation and Data were expressed as log number of Colony Forming Units (CFU) ml-1. The results of the study clearly indicate that most of the tested liquid formulations supported an adequate survival of rhizobial strains providing more than 108 bacteria ml-1 at the end of the experiment. Liquid inoculants formulated with Gum Arabic at 0.1%, 0.5% and 0.8% promoted long term survival of ENRRI 1, ENRRI 12 and TAL 380 while PVP at 0.1% supported the growth of strain USDA 110. The selected appropriate concentrations of the polymers for each rhizobial strain and isolate were then recommended for further studies concerning their uses as liquid inoculants. Few concentrations tended to slightly reduce cell density when compared to growth in YEM media. The study confirms the better survival of rhizobial cells in liquid inoculants amended with different concentrations of polymeric additives compared to liquid broth medium during initial growth. In addition, a degree of interaction between strains of rhizobia and polymeric additives was also noticed.


Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Assessment of some polymers for the formulation of rhizobial liquid inoculants

Albareda M, Rodríguez-Navarro DN, Camacho M, Temprano FJ. 2008. Alternatives to peat as a carrier for rhizobia inoculants: solid and liquid formulations. Soil Biology and Biochemistry 40, 2771-2779. https://doi.org/10.1016/j.soilbio.2008.07.021

Bashan Y, de-Bashan LE, Prabhu SR, Hernandez JP. 2013. Advances in plant growth-promoting bacterial inoculant technology: Formulations and practical perspectives (1998-2013). Plant and Soil 378, 1-33. https:// doi.org/10.1007/s11104-013-1956-x

Dayamani KJ, Brahmaprakash GP. 2014. Influence of form and Concentration of the Osmolytes in Liquid Inoculants Formulations of Plant Growth Promoting Bacteria. International Journal of Scientific and Research 4(7), 157-189

Deaker R, Roughley RJ, Kennedy I R. 2004.Legume seed inoculation technology-a review. Soil Biology and Biochemistry 36,1275-1288. https://doi.org/10.1016/j.soilbio.2004.04.009.

Demerlis CC, Schoneker DR. 2003. Review of the oral toxicity of polyvinyl alcohol (PVA). Food and Chemical Toxicology 41, 319-326. https://doi.org/ 10.1016/S0278-6915(02)00258-2.

Denardin ND, Freire, JRJ. 2000. Assessment of polymers for the formulation of legume inoculants.World Journal of Microbiology and Biotechnology 16, 215-217. https://doi.org/10.1023/ A:1008914223467

El Shafie AE, El Hussein AA. 1991. An evaluation of Rhizobium survival in two carriers new to Sudan. Experimental Agriculture 27, 319-321. https://doi. org/10.1017/S0014479700019037

Elsalahi RH, Mohamed SS, Sherif AM, Osman AG. 2016. Rhizobium Biofertilizer (Okadin) Production and Future Prospects in Sudan. Environment and Natural Resources International Journal 1(1), 01-12.

Errington JR, Pablo GD, Brian AP. 2002. The stability of proteins in a polyvinylpyrrolidone matrix. Department of chemical engineering, Princeton University USA.

Fernandes Júnior PI, Tiago Gusmão Rohr TG, De Oliveira PJ, Xavier GR, Rumjanek NG. 2009. Polymers as carriers for rhizobial inoculant formulations. Pesquisa Agropecuária Brasileira 44(9),1184-1190. http://dx.doi.org/10.1590/S0100-204X2009000900017

Gadalla AE, Migdam EA, Awad GO, Somaya SM, Belgees SA. 2010. Potential Production and Application of Biofertilizers in Sudan. Pakistan Journal of Nutrition 9(9), 926-934. http://dx.doi.org /10.3923/pjn.2010.926.934

Kavi Karunya S, Reetha D. 2014. Survival of saline tolerant PGPR in different carriers and liquid formulations. International Journal of Advanced Research in Biological Sciences 1(2), 179-183.

Leo Daniel Amalraj E, Maiyappan S, John Peter A. 2012. In vivo and in vitro studies of Bacillus megaterium var. phosphaticum on nutrient mobilization, antagonism and plant growth promoting traits. Journal of Ecobiotechnology 4, 35-42.

Mugnier J, Jung G. 1985.Survival of bacteria and fungi in relation to water activity and solvent properties of water in biopolymer. Applied Environmental Microbiology 50, 108-114.

Sarr A, Diop B, Peltier R, Neyra M, Lesueur D. 2005. Effect of rhizobial inoculation methods and host plant provenances on nodulation and growth of Acacia senegal and Acacia nilotica. New Forests 29, 75-87. https://doi.org/10.1007/s11056-004-5232-z

Singleton P, Keyser H, Sande E. 2002. Development and evaluation of liquid inoculants. Australian Centre for International Agricultural Research, Canberra 52-66.

Somasegaran P, Hoben HJ. 1994.Handbook for rhizobia, methods in legume-Rhizobium technology. New York: Springer-Verlag. https://doi.org/10.1007/ 978-1-4613-8375-8

Temprano FJ, Albareda M, Camacho M, Daza A, Santamaría C, et al. 2002. Survival of several Rhizobium/Bradyrhizobium strains on different inoculant formulations and inoculated seeds. International Microbiology 5, 81-86. https://doi.org/10.1007/ s10123-002-0067-y

Tittabutr P, Payakaponga W, Teaumroonga N, Singletonb W, Boonkerda N. 2007. Growth, Survival and Field Performance of Bradyrhizobial Liquid Inoculant Formulations with Polymeric Additives. Science Asia 33, 69-77. https://doi.org.10.2306/scienceasia1513-1874.2007.33.069

Valetti L, Angelini JG, Taurian T, Ibáñez FJ, Muñoz VL, Anzuay MS, Ludueña LM, Fabra A. 2016. Development and Field Evaluation of Liquid Inoculants with Native Bradyrhizobial strains for Peanut production. African Crop Science Journal 24(1), 1-13. http://dx.doi.org/10.4314/acsj.v24i1.1