Assessment of the crude ethanol leaf extracts of Momordica charantia Linn., Moringa oleifera Lam., Tabernaemontana pandacaqui Poir. and Mollugo oppositifolia Linn. for its wound healing activity in castrated piglets

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Research Paper 08/01/2024
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Assessment of the crude ethanol leaf extracts of Momordica charantia Linn., Moringa oleifera Lam., Tabernaemontana pandacaqui Poir. and Mollugo oppositifolia Linn. for its wound healing activity in castrated piglets

Jr. Priscilo P. Fontanilla, Angelina T. Gonzales
Int. J. Biosci. 24(1), 166-178, January 2024.
Copyright Statement: Copyright 2024; The Author(s).
License: CC BY-NC 4.0

Abstract

Botanicals possess healing and medicinal properties which could be an alternative to synthetic therapeutic drugs. The study evaluated the healing activity of selected botanicals on castration wounds of piglets. Crude ethanol leaf extracts of selected botanicals were subjected to wound healing activity assessment. Wound surface area, photography of wounds, epithelialization and scar appearance were assessed.  Results showed that all plants contain reducing sugars, tannins, carbohydrates, polyphenols, and flavonoids which may have contributed to wound healing activity independently or synergistically. Momordica charantia Linn., Moringa oleifera Lam., Tabernaemontana pandacaqui Poir. and Mollugo oppositifolia Linn. crude ethanol leaf extracts exhibited pro-healing activity by affecting the various phases of healing process, wound closure, and epithelialization of castration wounds of piglets. Tabernaemontana pandacaqui Poir. and Momordica charantia Linn. crude ethanol leaf extracts have the best wound healing potential compared to the other extracts. The crude ethanol leaf extracts have properties that render them capable of promoting accelerated wound healing activity. The use of botanicals in the treatment of castration wounds of piglets is far more economical than using commercial preparations.

Acha B, Serrano C, Acha J, Roa L. 2005. Segmentation and classification of burn images by color and texture information. Journal of Biomedical Optics 10(3), 034014.

Advanced Tissue. 2014. The Signs and stages of wound healing. Retrieved November 7, 2019 from https://advancedtissue.com/2014/09/signs-wound-healing-stages/

Agyare C, Bekoe E, Boakye Y, Dapaah S, Appiah T, Bekoe S. 2016. Medicinal plants and natural products with demonstrated wound healing properties. New insights into Ancient Challenges. IntechOpen. DOI: 10.5772/63574.

American Veterinary Medical Association. 2013. Literature review on the welfare implications of swine castration. Retrieved May 31, 2019 from https://www.avma.org/KB/Resources/LiteratureReviews/Pages/WelfareImplications-of Swine Castration .aspx

Aslam M, Ahmad M, Riaz H, Raza S, Hussain S, Qureshi O, Javed O. 2018. Role of flavonoids as wound healing agent. Intech Open. DOI:10.5772/intechopen.79179.

Barku V, Boye A, Ayaba S. 2013. Phytochemical screening and assessment of wound healing activity of the leaves of Anogeissus leiocarpus. Pelagia Research Library European Journal of Experimental Biology 3(4), 18-25.

Boakye Y, Agyare C, Ayande G, Titiloye N, Asiamah E, Danuah K. 2018. Assessment of wound-healing properties of medicinal plants: The case of Phyllanthus muellerianus. Frontiers in Pharmacology 9, 945. DOI: 10.3389/fphar. 2018.00945.

Bolla S, Al-Subaie A, Al-Jindan R, Balakrishna J, Ravi P, Veeraraghavan V, Surapaneni K. 2019. In vitro wound healing potency of methanolic leaf extract of Aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression. Heliyon 5(5).

Bowler P. 2003. Bacterial growth guideline: Reassessing its clinical relevance in wound healing. Wound management and prevention 49(1), 44-53.

Dahanukar A, Kulkarni A, Rege N. 2000. Pharmacology of medicinal plants and natural products. Indian Journal of Pharmacology 32, S81–118.

Drugs.com 2018. Furacin (Nitrofurazone topical). Retrieved August 11, 2019 from https://www.drugs.com/cons/furacin-topical.html

Enoch S, Price P. 2004. Cellular, molecular and biochemical differences in the pathophysiology of healing between acute wounds, chronic wounds and wounds in the aged. World of wounds. Retrieved November 6, 2019 from http://www.world widewounds.com/2004/august/Enoch/Pathophysiology-Of-Healing.html

Gushiken L, Hussni C, Bastos J, Rozza A, Padovani C, Takahira R, ellizon C. 2017. Hydroalcoholic extract from Copaifera langsdorffii has skin wound healing activity in rats. International Journal of Complementary & Alternative Medicine 6(1).

Hopf H, Kelly M, Shapshak D. 2008. Chapter 11 -Oxygen and the basic mechanisms of wound healing. Physiology and Medicine of Hyperbaric Oxygen Therapy. Retrieved October 25, 2019 from https://www.sciencedirect.com/topics/medicine-and-dentistry/epithelization

Houghton P, Kincaid C, Campbell K, Woodbury G, Keast D. 2000. Photographic assessment of the appearance of chronic pressure and leg ulcers. Ostomy Wound Management 46 (4), 21-23.

Humbert P, Meaune S, Gharbi T. 2004. Wound healing assessment. Phlebolymphology.org. Retrieved June 29, 2019 from https://www. phlebolymphology.org/wound-healing-assessment/

Leonard J. 2019. How to recognize and treat an infected wound. Medical news today. Retrieved November 7, 2019 from https://www.medical news today.com/articles/ 325040.php

Maynard J. 2015. How wounds heal: The 4 main phases of wound healing. Shield Health Care. Retrieved November 16, 2019 from http://www.shieldhealthcare.com/community/popular/2015/12/18/how-wounds-heal-the-4-main-hases-of-wound-healing/

Maver T, Kurečič M, Smrke D, Kleinschek K, Maver U. 2018. Plant-derived medicines with potential use in wound treatment. Herbal Medicine, Philip F. Builders, IntechOpen, DOI: 0.5772/intechopen.72813.

Mechesso A, Tadese A, Tesfaye R, Tamiru W, Eguale T. 2016. Experimental evaluation of wound healing activity of Croton macrostachyus in rat. African Journal of Pharmacy and Pharmacology 10(39), 832-838. DOI: 10.5897/AJPP2015.4454.

Mohan S, Sasikala K, Anand T. 2014. Antimicrobial and wound healing potential of Canthium coromandelicum leaf extract-a preliminary study. Research Journal of Phytochemistry 8 (2), 35-41. DOI: 10.3923/rjphyto.2014.35.41.

Padgett D, Marucha P, Sheridan J. 2007. Chapter 39 -Stress and wound healing: animal models. Psychoneuroimmunology (4th edition) 2, 837-850.

Ranade D, Collins N. 2014. Nutrition 411: An introduction to herbs for wound healing professionals. Wound Management and Prevention 60 (6), 1943-2720.

Shah A, Amini-Nik S. 2017. The role of phytochemicals in the inflammatory phase of wound healing. International Journal of Molecular Sciences 18(5), 1068. DOI:  10.3390/ijms18051068.

Singer A, Aora B, Dagum A, Valentine S, Hollander J. 2007. Development and validation of a novel scar evaluation scale. Plastic and Reconstructive Surgery 120(7) 1892-1897. DOI: 10.1097/01.prs.0000287275.15511.10

Thakur R, Jain N, Pathak R, Sandhu S. 2011. Practices in wound healing studies of plants. Evidence-Based Complementary and Alternative Medicine 2011, 17.

Thangapazham R, Sharad S,  Maheshwari R. 2016. Phytochemicals in wound healing. Advances in Wound Care 5(5), 230–241. DOI: 10.1089/ wound.2013.0505.

Wang J, Ruan J, Cai Y, Luo Q, Xu H, Wu Y. 2011. In vitro and in vivo evaluation of the wound healing properties of Siegesbeckia pubescens. Journal of Ethnopharmacology 134 (3), 1033–1038.

Zeng Q, Xie H, Song H, Nie F, Wang J, Chen D, Wang F. 2016. In vivo wound healing activity of Abrus cantoniensis extract. Evidence-Based Complementary and Alternative Medicine 2016, 7.

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