Assessment of the diuretic activity of Hydrocotyle vulgaris methanolic extract in Albino Mice
Paper Details
Assessment of the diuretic activity of Hydrocotyle vulgaris methanolic extract in Albino Mice
Abstract
This study assessed the diuretic potential of methanolic extract of Hydrocotyle vulgaris in albino mice after oral administration. Methanolic extract of H. vulgaris (40 and 20mg/kg) and the reference drug, Furosemide (20mg/kg) were administered intraperitoneally using the modified Lipschitz method. All animals were pretreated with saline before commencing the experiment. Their urine output and electrolyte changes were quantitated at several intervals of time after the dose of 5 hours and after 24 hours. The urine output increased significantly in Furosemide and in both 40 and 20mg/kg extracts (p<0.05). Methanolic extract of H. vulgaris increased the urine volume and electrolyte balance in a dose-dependent manner and qualitatively similar to that of Furosemide. These findings collectively indicate that the extract exhibited significant diuretic activity, providing evidence, at least in part, for its traditional use as a diuretic.
Alvarez M, Ramirez C, Deil U. 2018. Ecology and distribution of Hydrocotyle Cryptocarpa speg. in South America. Gayana Botanica 65(2), 139-144.
Braker J, Borriss RP, Carte B, Cordell G, Soejarto D, Cragg G, Tyler V. 1995. Natural product drug discovery and development: New perspectives on international collaboration. Journal of Natural Products, 58(9), 1325-1357.
Burton J. 2002. Online guide to Umbelliferae of British Isles. Hydrocotyle vulgaris (L) Marsh pennywort. Retrieved from www.spookspring.com/Umbels/Hydro.html
Evans J. 2002. The effect of local resource availability and clonal integration on ramet functional morphology in Hydrocotyle bonariensis. Oecologia 89, 265-276.
Garcia Matilla F, Garcia ontes F, Ribas Serna J. 1999. Relaciones entre diuresis, pH de la orina y litogénesis [Relationships between diuresis, urine pH and lithogenesis]. Actas urologicas espanolas 23(3), 202-213.
Hullati K, Sharada M, Kuppasth I. 2011. Studies on diuretic activity of three plants from Menispermaceae family. Der Pharmacia Sinica 2(1), 129-134.
Jacoby D, Youngson R. 2005. Encyclopedia of Family Health. 3rd ed. Retrieved from New York: Marshall Cavendish Corporation: http://books.google
James J, Dubery I. 2009. Pentacyclic triterpenoids from the medicinal herb, Centella asiatica (L.) Urban. Molecules 14, 3922-3941.
Leong T, Chooi O, Siong Hock A. 2009. Bioassay guided isolation of cytotoxic compounds from Hydrocotyle vulgaris. Malaysian Journal of Biochemistry and Molecular Biology 17(1), 32.
Mazid M, Datta B, Nahar L, Rashid A, Bachar S, Khairul Bashar S, Sarker S. 2009. Antinociceptive, anti-inflammatory and diuretic properties of Polygonum barbatum (L.) Hara var. barbata. Retrieved from Rev. bras. farmacogn: http://www.scielo.br/scielo
Mazid M, Datta B, Nahar L, Rashid A, Bachar S, Khairul Bashar S, Sarker S. 2010. Analgesic and diuretic properties of α-santalone from Polygonum flaccidum. Phytotherapy Research 24, 1084-1087.
Patel U, Kulkarni M, Undale V, Bhosale A. 2009. Evaluation of diuretic activity of aqueous and methanol extracts of Lepidium sativum Garden Cress (Cruciferae) in rats. Tropical Journal of Pharmaceutical Research 8(3), 215-219.
Sadki C, Hacht B, Souliman A, Atmani F. 2010. Acute diuretic activity of aqueous Erica multiflora flowers and Cynodon dactylon rhizomes extracts in rats. Journal of Ethnopharmacology 128, 352-356.
Sangita S, Rashmika P, Rajiv, Kukkar. 2009. Study of phytochemical and diuretic potential of methanol and aqueous extracts of aerial parts of Phyla nodiflora Linn. International Journal of Pharmacy and Pharmaceutical Sciences 1.
Shinkawa T, Yamasaki F, Kikuchi A, Nakakuki M, Nishjima K, Uemura A, Orita Y. 1992. Pharmacological properties of the novel highly potent diuretic 7-chloro-2,3-dihydro-1-(2-methylbenzoyl)- 4(1H)-quinolinone 4-oxime-O-sulfonic acid potassium salt. Arzneimittelforschung 42(12),1466-72.
Singh S, Asmita G, Abhimanyu S, Amla B. 2010. A plant with immense medicinal potential but threatened. International Journal of Pharmacuetical Sciences Review and Research 4(2), 3.
Taylor L. 2000. Plant based drugs and medicines . Retrieved from The Healing Power of Rainforest Herbs: http://www.rain-tree.com/plantdrugs.htm.
Tor M. 2010. How to grow Hydrocotyle herbs. Retrieved from http://www.ehow.com/how_6573675_grow-hydrocotyle-herbs.html.
Vogel H. 2006. Drug discovery and evaluation: safety and pharmacokinetic assays. Retrieved from New York: Springer-Verlag Berlin Heidelberg: http://books.google.com.ph.
MA. Chrischelle F. Bullecer, 2022. Assessment of the diuretic activity of Hydrocotyle vulgaris methanolic extract in Albino Mice. J. Biodiv. Environ. Sci., 21(1), 10-15.
Copyright © 2022 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.