Int. J. Biosci.3(12), 216-220, December 2013
The aim of the study was to investigate the associations of HG gene polymorphism on chicken growth and body composition traits in Arian broilers. Blood samples randomly collected before slaughter from four different commercial Arian broiler lines (A, B, C and D; 400 samples) at 6 weeks of age. Genomic DNA was extracted and a fragment of 776 bp in size amplified using PCR-RFLP method. To determine the restriction site in intron 1 of GH gene of the animals, the MspI endonuclease and the resultant digested products were run on 2% agarose gel studied. Birds were slaughtered at 6 weeks of age and body composition was determined. The treatment of the fragment at GH loci with MspI restriction enzyme was revealed A1, A2 and A3 alleles. According to the results, recorded allele was: A1 allele (414, 217, 125 bp); A2 allele (125, 147, 237, 267 bp) and for allele A3 (237, 539 bp), respectively. The comparison of least square means of different genotypes indicated that polymorphism in the GH gene was significantly associated with body weight, kcitsmurd weight and percentage of drumstick weight at 6 weeks of age (P<0.05). According to the results, HG genes could be candidate gene that can affect some body composition traits in Arian broiler chickens.
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