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Bacillus thuringiensis berliner cells population growth in some naturally media and the patogenicity against Plutella xylostella Caterpilars

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Research Paper 01/02/2019
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Bacillus thuringiensis berliner cells population growth in some naturally media and the patogenicity against Plutella xylostella Caterpilars

Akhmad Gazali, Achmad Jaelani, Ilhamiyah Ilhamiyah, Siti Erlina
Int. J. Biosci.14( 2), 209-215, February 2019.
Certificate: IJB 2019 [Generate Certificate]
Key: Bacillus thuringiensis BerlinerDiamondback mothMixture extract mediaPlutella xylostellaPopulation growth

Abstract

Bacillus thuringiensis is an important biological control agent in nature. For farmers to utilize B.  thuringiensis, it needs to be researched in media where it can be reproduced easily, quickly, and produce many cells with high pathogenicity. The study aimed to test the influence of some propagation media on the growth of B.  thuringiensis Berliner cell populations that have a high pathogenicity and compares the pathogenicity between B. thuringiensis propagated in some media against Plutella  xylostella caterpillars. Bacillus thuringiensis isolates were taken from the tidal land on the islands of Borneo.  The research used a completely randomized design with five treatments a) J Media (corn extract; b) K Media (soybean extract); c) B Media (rice extract); d) C Media (extract mixture of corn, soybean, and rice ratio of 1: 1: 1); and e) Nutrien Broth Media (NB Media) and four replications. The parameter measured was the number of cells. Pathogenicity test between B. thuringiensis that is propagated in some media against P. xylostella caterpillars were determined by probit analysis.  The results showed that, growth media that produced the most B. thuringiensis cells are J Media (corn extract), B  Media (extract of rice), and C Media (extract mixture of rice, corn, and soybeans). The highest pathogenicity of B.  thuringiensis which is propagated in the C Media (extract mixture of corn, rice and soybeans) with LC50 value is 7.96 × 105 cells/ml suspension. The media type which produce the most B. thuringiensis cells and have the highest pathogenicity is C media (extract mixture of corn, rice and soybeans).

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Abdelkefi-Mesrati  L, Boukedi H, Dammak-Karray M, Sellami-Boudawara T, Jaoua S,  Tounsi S.   2011.   Study of the Bacillus thuringiensis Vip3Aa16 histopathological effects and determination of its putative binding proteins in the midgut of Spodoptera littoralis. Journal of Invertebrate Pathology 106, 250–254.

Berbert-Molina MA, Prata AMR, Pessanha LG, Silveira MM. 2008. Kinetics of Bacillus thuringiensis var. israelensis growth on high glucose concentrations. Journal of  Industrial Microbiology and Biotechnology  35, 1397–1404.

Bhowmik A, Mourin M, Shishir A, Khan SN, Hoq M. 2015.  Development of a Cost Effective Medium for Enhanced Production of Bacillus thuringiensis δ -endotoxin 32, 1–6.

Chen ML, Chen PH, Pang JC, Lin CW, Hwang CF, Tsen HY. 2014.  The correlation of the presence and expression levels of cry genes with the insecticidal activities against Plutella xylostella for Bacillus thuringiensis Strains, Toxins 6, 2453–2470. http://dx.doi.org/10.3390/toxins6082453.

Deist BR, Rausch MA, Fernandez-Luna MT, Adang MJ, Bonning BC. 2014.  Bt toxin modification for enhanced efficacy, Toxins 6, 3005–3027. http://dx.doi.org/10.3390/toxins6103005.

Gazali A, Ilhamiyah, Jaelani A. 2015.  Patogenicity of Bacillus thuringiensis which Isolated from Tidal Ecosystem against Diamond Backmoth Larvae , Plutella xylostella Linn, Asian Journal of Applied Sciences 03, 513–518.

Gazali A, Ilhamiyah, Jaelani A. 2017a. Bacillus thuringiensis: Biologi, Isolasi, Perbanyakan dan Cara Aplikasinya. 1st edn. Banjarmasin: Pustaka Banua. Available at: http://eprints.ulm.ac.id/eprint/4082.

Gazali A, Ilhamiyah, Jaelani A. 2017b.  Agroecosystem Stability and Breakdown Leaves on Mustard Cropping after Application by the Bacillus thuringiensis, International Journal of Science and Research (IJSR) 6, 433–437. http://dx.doi.org/10.21275/ART20172344.

Guidelli-thuler AM, Abreu ILD, Victor M, Lemos F.  2009.  Expression of the  Sigma 35 and Cry2ab Genes Involved in Bacillus thuringiensis  Virulence, (June) p 403–409.

Hoa NT. 2014. Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus thuringiensis Delta Endotoxin Production’, American Journal of Agriculture and Forestry 2, p. 219. http://dx.doi.org/10.11648/j.ajaf.20140205.12.

Van Der Hoeven N, Vijver EMG, Van Straalen NM. 2014.  Bacillus thuringiensis toxins: Their mode of action and the potential interaction between them, Ecostat,  1–186. http://dx.doi.org/10.4161/bbug.1.1.10519.

Höfte H, Whiteley HR. 1989.  Insecticidal crystal proteins of Bacillus thuringiensis. Microbiological reviews 53, 242–255.

Marzban R. 2012.  Investigation on the suitable isolate and medium for production of Bacillus thuringiensis, Journal of Biopesticides 5, 144–147.

Nadu T. 2015. Combined effect of Bacillus thuringiensis and Bacillus subtilis against Helicoverpa armigera’, International Journal of current Micobiology and Applied Science 4, 127–141.

Soccol CR, Pollom TEV, Fendrich RC, Prochmann FA, Mohan R, Blaskowski MMM, Melo ALA, Carvalho CJB,  Soccol VT.  2009. Brazilian Archives of Biology and Technology:  Development of a Low Cost Bioprocess for Endotoxin Production by Bacillus thuringiensis var israelensis Intended for Biological Control of Aedes aegypti, Arch. Biol. Technol 52, 121–130.

Sedlak M, Walter T, Aronson A. 2000. Regulation by overlapping promoters of the rate of synthesis and deposition into crystalline inclusions of Bacillus thuringiensis δ- endotoxins, Journal of Bacteriology 182, 734–741. http://dx.doi.org/10.1128/JB.182.3.734-741.2000.

Travers RS, Martin PAW, Reichelderfer CF. 1987.  Selective Process for Efficient Isolation of Soil Bacillus spp, Applied and Environmental Microbiology  53, 1263–1266.

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