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Bacillus thuringiensis berliner cells population growth in some naturally media and the patogenicity against Plutella xylostella Caterpilars

Akhmad Gazali, Achmad Jaelani, Ilhamiyah Ilhamiyah, Siti Erlina

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Int. J. Biosci.14(2), 209-215, February 2019

DOI: http://dx.doi.org/10.12692/ijb/14.2.209-215


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Bacillus thuringiensis is an important biological control agent in nature. For farmers to utilize B.  thuringiensis, it needs to be researched in media where it can be reproduced easily, quickly, and produce many cells with high pathogenicity. The study aimed to test the influence of some propagation media on the growth of B.  thuringiensis Berliner cell populations that have a high pathogenicity and compares the pathogenicity between B. thuringiensis propagated in some media against Plutella  xylostella caterpillars. Bacillus thuringiensis isolates were taken from the tidal land on the islands of Borneo.  The research used a completely randomized design with five treatments a) J Media (corn extract; b) K Media (soybean extract); c) B Media (rice extract); d) C Media (extract mixture of corn, soybean, and rice ratio of 1: 1: 1); and e) Nutrien Broth Media (NB Media) and four replications. The parameter measured was the number of cells. Pathogenicity test between B. thuringiensis that is propagated in some media against P. xylostella caterpillars were determined by probit analysis.  The results showed that, growth media that produced the most B. thuringiensis cells are J Media (corn extract), B  Media (extract of rice), and C Media (extract mixture of rice, corn, and soybeans). The highest pathogenicity of B.  thuringiensis which is propagated in the C Media (extract mixture of corn, rice and soybeans) with LC50 value is 7.96 × 105 cells/ml suspension. The media type which produce the most B. thuringiensis cells and have the highest pathogenicity is C media (extract mixture of corn, rice and soybeans).


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Bacillus thuringiensis berliner cells population growth in some naturally media and the patogenicity against Plutella xylostella Caterpilars

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