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Bacteriological profile and antibiogram of blood culture isolates from patients of rural tertiary care hospital

Research Paper | October 1, 2016

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S. Oza Sweta, J. Mehta Sanjay, M. Kunjan Kikani, G. Oza Sunil

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Int. J. Micro. Myco.4( 3), 1-7, October 2016


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Microbial invasion of blood stream is associated with significant mortality and morbidity. Identification of bacterial isolates and antibiotic susceptibility of bacteria isolated from blood culture would guide the antibiotics treatment for patients with bacteremia. 1) To determine age – wise blood culture positivity rate in bacteremia 2) To identify age – wise common bacterial species isolates in bacteremia 3) To determine Antibiotic sensitivity pattern of the bacterial isolates. Atotal of 247 blood culture samples received from various clinical departments of rural teaching hospital from August 2013 to September 2015 were included in the study. Samples were collected in brain heart infusion broth. Identification of isolates and antimicrobial susceptibility was done as per standard microbiological methods. Out of 247 specimens bacteria sp. was isolated from 46 (18.62%) samples. Blood culture positivity was noted highest among neonates age group (38.71%). Lowest rate was observed among elders (4.55%). Klebsiella pneumoniae, Coagulase negative staphylococcus (CONs), and S. aureus were common blood culture isolates. In neonates Klebsiella pneumoniae was the most common isolate. Out of 27 gram negative bacilli, 14 (51.85%) were extended spectrum betalactamases (ESBL) positive. High resistance was noted against amoxycillin and amoxicillin/clavulanic acid and third generation cephalosporins in all gram negative organisms except, S. typhi. Out of 12 Staphylococcus sp., none of these were methicillin resistant. Routine antibiotic susceptibility surveillance helps in choice of antibiotics for treatment, identification of resistance and control of its spread.


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Bacteriological profile and antibiogram of blood culture isolates from patients of rural tertiary care hospital

Agarwal A, Bhat S. 2015. Clinico-microbial study of neonatal sepsis. J of International Medicine and Dentistry 2(1), 22-9.

Arpi M, Victor MA, Moller JK, Jonsson V, Hansen MM, Peterslund NA, Bruun B. 1994. Changing etiology of bacteremia in patients with hematological malign-ancies in Denmark. Scand J Infect Dis 26, 157-62.

Betty AF, Daniel FS, Alice SW. 2007. Bailey and Scott’s diagnostic microbiology: Wilson L, Wurm E, editors. Blood stream infections. 12th ed. China: Elsevier publisher; 778-84.

Cockerill FR, Patel JB, Alder JG, Bradford PA, David MN, Hardy DJ et al. CLSI Performance standards for Antimicrobial susceptibility testing; Twenty-third Informational Supplement, Approved standard-11th edition, NCCLS Publishers, Wayne PA, M100-S 23 (January 2013).

Cohen ML. 1997. Epidemiological factors influencing the emergence of antimicrobial resistance. Ciba Found Symp 20, 223-31.

Collee JG, Miles RS, Watt B. 1996. Tests for the identification of bacteria. In: Collee JG, Fraser AG, Marmion BP, Simmons A, editors. Mackie and McCartney Practical Medical Microbiology. 14th Ed. London: Livingstone 131-49.

Collee JG, Miles RS, Watt B. Mackie & McCartney Practical Medical Microbiology: Collee JG, Fraser AG, Marmion BP, Simmons A, editors. 2006. Tests for the identification of bacteria. 14th ed. Elsevier Publisher: Rajkamal electric press 121-3.

Meremkwer MM, Nwachukwu CE, Asuquo AE, Okebe J, Utsalo SJ. 2005. Bacterial isolates from blood cultures of children with suspected septicaemia in Calabar, Nigeria. BMC Infect Dis 5, 110-5.

Onipede AO, Onayade AA, Elusiyan JB, Obiajunwa PO, Ogundare EO, Olaniran OO et al. 2009. Invasive bacteria isolates from children with severe infections in a Nigerian hospital. J Infect DevCtries 3, 429-36.

Prabhu K, Bhat S, Rao S. 2010. Bacteriologic profile and antibiogram of blood culture isolates in a pediatric care unit. J laboratory physicians 2(2), 85-8.

Reacher MH, Shah A, Livermore DM, Wale MC, Graham C, Johnson AP et al. 2000. Bacteraemia and antibiotic resistance of its pathogens reported in England and Wales between 1990 and 1998: Trend analysis. BMJ 320(7229), 213-6.

Roy I, Jain A, Kumar M, Agarwal SK. 2002. Bacteriology of neonatal septicaemia in a tertiary care hospital of northern India. Indian J Med Microbiol 20,156-9.

Saraswathi DA,  Gogate KA, Fernandes  AR. 1995. Bacteremia in hospitalized children-A one year prospective study.Indian J Med Microbiol 13, 72-5.

Shrestha S, Amatya R, Shrestha R, Shrestha R. 2014. frequency of blood culture isolates and their antibiogram in a teaching hospital. J Nepal Med Assoc 52(193), 692-6.

Sobhani A, Shodjai H, Javanbhakht S. 2004. Drug resistance pattern in isolated bacteria from blood culture. Actamedicairanica 42(1), 46-9.

Thomson KS, Sanders CC. 1992. Detection of extended spectrum beta lactamases in members of family enterobacteriaceae-comparison of double disk and 3D test. Antimicrob Agents Chemother 36, 1877-82.