Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | October 1, 2016

VIEWS 3
| Download 18

Bio-efficacy of Pseudomonas fluorescens isolated from chickpea fields as plant growth promoting rhizobacteria

Shazia Shahzaman, Muhammad Inam-Ul-Haq, Shagufta Bibi, Muhammad Sufyan, Adeela Altaf, Umair Mehmood, Raees Ahmed

Key Words:


Int. J. Biosci.9(4), 138-146, October 2016

DOI: http://dx.doi.org/10.12692/ijb/9.4.138-146

Certification:

IJB 2016 [Generate Certificate]

Abstract

Chickpea is an economically important food crop, which is subjected to infection by a host of fungal, viral and bacterial pathogens. Thirty isolates of Pseudomonas fluorescens were isolated from the rhizosphere of Chickpea fields. These were tested against F. oxysporum in dual culture method. Among these, four (Pf 1, Pf 3, Pf 5 and Pf 8) isolates were showed bright fluorescence under UV light were further tested. All the cultural and biochemical studies confirmed them to be P. fluorescens. The isolates also showed positive response for siderophore production and plant growth promoting activity on Chickpea cultivar Bital 98. Among these isolates Pf 3 and Pf 5 shown significant results by increasing root length and shoot length. Both the Pf 3 and Pf 5 isolates were found significantly superior than other isolates in increasing the shoot length (12.7 cm) and root length (24.5 cm) over control. The isolates Pf 3 was recorded high vigor index (3830) followed by Pf 5 (3648). The least vigor index was recorded by Pf 1 (2631).

VIEWS 3

Copyright © 2016
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Bio-efficacy of Pseudomonas fluorescens isolated from chickpea fields as plant growth promoting rhizobacteria

Angayarkanni T, Kamalakannan A, Santhini E, Predeepa D. 2005. Identification of biochemical markers for the selection of Pseudomonas fluorescens against Pythium spp. In: Asian conference on Emerging Trends in Plant-Miceobial Interactions. University of Madras, Chennai. 295-303 p.

Anija KR. 1996. 2nd experiments in Microbiology, Plant pathology Tissue Culture and Mushroom Cultivaiton. New Delhi Vishwa Prakashan. 14(1): 11-234.

Anuratha CS, Gnanamanickam SS. 1990. Biological control of bacterial wilt caused by pseudomonas solanacearum in India with anatagonistic bacteria. Plant Soil 124(2), 109-116. http://dx.doi.org/10.1007/BF00010938

Baki AA, Anderson JD. 1973. Vigour determination in soybean seeds by multiple criteria. Crop Science 13(2), 630-633. http://dx.doi.org/10.2135/cropsci1973.0011183X001300060013x

Balasubramanian R. 1994. Biological control of Chickpeablast: Role of antifungal antibiotic in disease suppression. Ph.D. Dissertation, University of Madras, Madras.

Buchannan RE, Gibbson NE. 1974. Bergeys manual of determinative bacteriology. 8th Ed. Williams and Wilkins Co. Baltimore.

Buyer TS, Leong J. 1986. Iron transport mediated antagonism between plant growth promoting and plant deleterious Pseudomonas strains. Journal of Biological Chemistry 261(2), 791-94.

Gnanamanickam SS, Mew TW. 1992. Biological control of blast disease of Chickpea (Oryza sativa L.) with antagonistic bacteria and its mediation by a Pseusomonas antibiotic. Annual Phytopathological Society Japan 58(1), 380-385.

Harrigen WF, Margeret E. 1966. Laboratory methods in Microbiology. Academic Press.

King EO, Wood MK, Raney DE. 1954. Two simple media for the demonstration of pyocyanin and luorescein. Journal of Laboratory Clinical Medicine. 44(2), 301-307.

Leong J. 1986. Siderophore: Their biochemistry and possible role in the biocontrol of plant pathogens. Annual Review of Phytopathology 24(2), 187-209. http://dx.doi.org/10.1146/annurev.py.24.090186.001155

Mew TW, Rosales AM. 1986. Bacterization of Chickpea plants for control of sheath blight caused by Rhizoctonia solani. Phytopathology 76(2), 1260-1264. http://dx.doi.org/10.1094/Phyto-76-1260

Mortan DJ, Stroube WH. 1955. Antagonistic and stimulatory effect of soil microorganism upon Sclerotium. Phytopathology 45(2), 417-420.

Nathan P, Rathinam X, Kasi M, Abdul Rahman Z, Subramanian S. 2011. A pilot study on the isolation and biochemical characterization of Pseudomonas from chemical intensive rice ecosystem. African journal of Biotechnology Vol. 10(59), 12653-12656. http://dx.doi.org/10.5897/AJB10.1819

Neilands JB. 1981. Microbial iron compounds. Annual review of Biochemistry 50(2), 712-31. http://dx.doi.org/10.1146/annurev.bi.50.070181.003435

O’Sullivan DJ, O’Gara F. 1992. Traits of fluorescent Pseudomonas spp. Involved in suppression of plant root pathogen. Microbiology Review 56(2), 662-626.

Ramanathan A, Shanmugam V, Raguchander T, Samiyappan R. 2002. Induction of systemic resistance in ragi against blast disease by Pseudomonas fluorescens. Annual Plant Protection Society 10(2), 313-318.

Sakthivel N, Gnanamanickam SS. 1887. Evaluation of Pseudomonas fluorescens for suppression of sheath rot disease and for enhancement of grain yields in rice (Oryza sativa L.) Applied Environmental Microbiology 53(1), 2056-2059.

Schwyan B, Neiland JB. 1987. University chemical assay for detection and determination of siderophores. Annual Biochemistry 60(2), 47-56. http://dx.doi.org/10.1016/0003-2697(87)90612-9

Sivamani E, Gnanamanickam SS. 1988. Biological control of Fusarium oxysporum f. sp. cubense in banana by inoculation with Pseudomonas fluroscense. Plant soil 107(2), 3-9. http://dx.doi.org/10.1007/BF02371537

Thara KV, Gnanamanickam SS. 1994. Biological control of sheath blight in India: lack of correlation between chitinase production by bacterial antagonists and sheath blight suppression. Plant soil 160(1), 277-280. http://dx.doi.org/10.1007/BF00010153

Todar K. 2004. Pseudomonas and related bacteria. Todar’s online text book of bacteriology. http://textbookofbacteriology.net/Pseudomonas.etc.

SUBMIT MANUSCRIPT

Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background