Bioactive agents of Origanum vulgare against Staphylococcus haemolyticus

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Research Paper 01/12/2020
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Bioactive agents of Origanum vulgare against Staphylococcus haemolyticus

Javeria Zaheer, Sehr Syed, Fiaz Ahmed, Kainat Riaz, Sadia Mushtaq, Madiha Riaz, Aimen Aisha, Muhammad Anas Aslam, Maryam Khan, Saba Shamim
Int. J. Biosci.17( 6), 305-316, December 2020.
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There are several bacterial infections that are associated with the use of medical devices in patients. Compromised immune system tend to play an important role in the pathogenesis of disease. In this research work, the microbial flora of medical devices of the patients being treated at OPD (Out Patient Door) wards of three local tertiary care hospitals was investigated. After the morphological, biochemical, and molecular characterization of the most common bacterial isolate, it was found to be Staphylococcus haemolyticus. For the determination of antibacterial and bioactive potential of O. vulgare, its extracts were prepared and examined for their antibacterial potential. The results demonstrated that the methanolic extract was the most effective against S. haemolyticus. It was selected on this basis for further analysis. The suppression of proteins in case of both extracts was also observed during the quantitative and qualitative analysis of proteins by Bradford assay and SDS-PAGE, respectively. The phytochemical analysis of various compounds of the methanolic extract comprised of the Thin Layer Chromatography (TLC) and GC/MS analysis, respectively. TLC demonstrated the presence of compounds of Rf values of 0.9 and 0.81, respectively. The GC/MS analysis of O. vulgare extract revealed the presence of 12 phytochemical compounds in the methanolic extracts.


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