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Bioactive compounds proximate composition and cytotoxicity of Echinoidea (Sea Urchin): Basis for future policy preservation and conservation

Genevieve U Garcia, Nenita D Palmes, Oliva P Canencia

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Int. J. Biosci.14(4), 349-358, April 2019

DOI: http://dx.doi.org/10.12692/ijb/14.4.349-358


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The presence of Echinoidea in marine ecosystem at the inter-tidal zone of the Philippines is abundant. The proximate composition and cytotoxicity of the combined spines, tests and gonads of the selected three species of sea urchin namely: Diadema setusom, Tripnuestes gratilla and Astropyga radiata greatly vary in their proximate composition. Specifically, the three-sample species are potential source of nutrients and minerals. The cytotoxic activity using brine shrimp lethality test differ in selected sea urchins. The test for cytotoxicity showed that LC50 is ˃1000 ppm which is non-toxic. The results confirmed samples non-toxicity since the species are edible and local folks eat and sell them as source of livelihood. Preservation and conservation of sea urchin species are necessary for it is one of the most significant marine invertebrates used as bioindicator and as source for livelihood and commercialization.


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Bioactive compounds proximate composition and cytotoxicity of Echinoidea (Sea Urchin): Basis for future policy preservation and conservation

Arafa S, Chouaibi M, Sadok S, El Abed A. 2012. The influence of season on the gonad index and biochemical composition of the sea urchin Paracentrotus lividus from the Gulf of Tunis. Scientific World Journal p. 1- 8.

Association of Analytical Chemists (AOAC). 2000. In: 15th Ed. Official methods of analysis. Washington DC, USA 141-144.

Barbarino E, Lourenço S. 2009. Comparison of CHN analysis and Hach acid digestion to quantify total nitrogen in marine organisms. Limnology and Oceanography: Methods 7, 751-760. https://doi.org

Bragadeeswara S, Kumaran NS, Sankar PP, Prabahar R. 2013. Bioactive potential of sea urchin Temnopleurus toreumaticus from Devanampattinam, Southeast coast of India. Journal of Pharmacy and Alternative Medicine 3, 9-18.

Chiarelli R, Roccheri MC. 2014. Marine invertebrates as bioindicators of heavy metal pollution. Open Journal of Metal 4, 93-106. https://doi.org/10.4236/ojmetal.2014.44011

Clarkson C, Maharaj VJ, Crouch NR, Grace OM, Pillay P, Matsabisa M Bhagwandin N, Smith PJ, Folb PI. 2004. In vitro antiplasmodial activity of medicinal plants native to or naturalized in South Africa. Journal of Ethnopharmacology 92, 177-191.

Department of Environment and Natural Resources (DENR). 2002. PBCP-Phil Biodiversity Conservation Priorities. Quezon City, Philippines.

Diniz GS, Barbarino E, Oiano-neto J, Pacheco S, Lourenço SO. 2014. Proximate composition of marine invertebrates from tropical coastal waters , with emphasis on the relationship between nitrogen and protein contents. Latin American Journal of Aquatic Research 2, 332-352.https://doi.org/10.3856

Finney DJ. 1952. Probit Analysis. Journal of the Institute of Actuaries 3, 388-390. https://doi.org/10.1017

Ghazarian H, Idoni B, Oppenheimer S. 2012. A glycobiology review: carbohydrates, lectins, and implications in cancer therapeutics. National Institute of Health 3, 236-247. https://doi.org/10.1016/

Gonzales BJ. 2005. Guide to species selection and principles of marine stock enhancement in the Philippines.Fisheries Resource Management Project. Quezon City, Philippines 1-27.

Hughes TP, Bellwood DR, Folke, Steneck RS, Wilson J. 2005. New paradigms for supporting the resilience of marine ecosystems. Trends in Ecology and Evolution 7, 381-386. https://doi.org/

Ibañez E, Herrero M, Mendiola JA, Castro-puyana M. 2012. Extraction and characterization of bioactive compounds with health benefits from marine resources : macro and micro algae, cyanobacteria, and invertebrates. M. Hayes (ed.), Marine Bioactive Compounds: Sources, Characterization and Applications. Madrid, Spain: Springer Science+Business Media 55-98. https://doi.org/10.1007

Jinadasa BKKK, De Zoysa HKS, Jayasinghe GDTM, Edirisinghe EMRKB. 2016. Determination of the biometrical parameters, biochemical composition and essential trace metals of edible sea urchin (Stomopneustes variolaris) in Sri Lanka. Cogent Food & Agriculture 2, 1-12. https://doi.org/10.1080

Lopes VR, Fernández N, Martins RF, Vasconcelos V. 2010. Primary screening of the bioactivity of brackishwater cyanobacteria: Toxicity of crude extracts to Artemia salina larvae and Paracentrotus lividus embryos. Marine Drugs 3, 471-482. https://doi.org/10.3390/md8030471

Lukyanova ON, Zhuravel EV, Chulchekov DN, Mazur AA. 2017. Sea urchin embryogenesis as bioindicators of marine pollution in impact areas of the sea of Japan / East Sea and the Sea of Okhotsk. Archives of Environmental Contamination and Toxicology 2, 322-333.

Meyer B, Ferrigni N, Putnam J, Jacobse L, Nichols D, McLaughlin J. 1982. BrineShrimp: A Convenient General Bioassay for Active Plant Constituents. Planta Medica 5, 31-34. https://doi.org

Michio K, Kengo K, Yasunori K, Hitoshi M, Takayuki Y, Hideaki Y, Hiroshi S. 2003. Elects of deposit feeder Stichopus japonicus on algal bloom and organic matter contents of bottom sediments of the enclosed sea. Marine Pollution Bulletin 47, 118-125.

Mills SC, Peyrot-Clausade M, Fontaine MF. 2000. Ingestion and transformation of algal turf by Echinometra mathaei on Tiahura fringing reef (French Polynesia). Journal of Experimental Marine and Ecology 1, 71-84. https://doi.org/10.4049

Mol S, Baygar T, Var-Lik C, Tosun SY. 2008. Seasonal variations in yield, fatty acids, amino acids and proximate compositions of sea urchin (Paracentrotus lividus) roe. Journal of Food and Drug Analysis 2, 68-74.

Mooi R, Munguia A. 2014. Sea urchins of the Philippines. In: California Academy of Sciences Ed. The coral triangle: The 2011 Hearst Philippine Biodiversity Expedition. California, USA: Research Gate 213-235. https://doi.org/10.4049

Nievales MF, Juinio-Meñez MA, Bangi HG. 2006. Status and Prospects of Aquaculture of Threatened Echinoderms in the Philippines for Stock Enhancement and Restocking. Southeast Asian Fisheries Development Center (SEAFDEC). Iloilo, Philippines, 61-69.

Pates ALA, Responte JAA, Mag-aso AV, Dagoc KMF. 2015. Density of Tripneustesgratilla (Linnaeus, 1758) in the coastal area of Binuni, Lanao Del Norte: Potentials for sea urchin grow-out culture. Advances in Environmental Biology 9, 171-175.

Primavera JH. 2005. Mangroves and aquaculture in Southeast Asia. In: Aquaculture Department, Southeast Asian Fisheries Development Center (SEAFDEC). Iloilo, Philippines 61-69.

Rahman MA, Arshad A, Yusoff FM. 2014. Sea urchins (Echinodermata:Echinoidea): Their biology, culture and bioactive compounds. Ecological and Medica Sciences 39-48. https://doi.org/10.15242

Salma WO, Hafizah I, Nurtamin T, Haya LOM, Suryani A. 2016. The potential gonad of Diadema setosum as a healthy food to improve the nutritional status of coastal’s children. International Journal of Sciences: Basic and Applied Research 2, 189-195.

Schoppe S. 2000. Echinoderms of the Philippines : a guide to common shallow water sea stars, brittle stars, sea urchins, sea cucumbers and feather stars. In:VISCA-GTZProgram on Applied Tropical Ecology, Visayas Sate College of Agriculture. Singapore: Times Edition 137-144.

Tenuzzo BA, Carata E, Mariano S, Dini L. 2017. Morphological and biochemical profiles of the gonadal cycle in the sea urchin Paracentrotus lividus: Wild type vs. bred. In: Sea Urchin – From Environment to Aquaculture and Biomedicine. Lecce, Italy: Licensee In Tech, 73-91. https://doi.org

Thompson RJ, Macdonald BA. 1990. The role of environmental conditions in the seasonal synthesis and utilisation of biochemical energy reserves in the giant scallop, Placopecten magellanicus. Can. J. Zool 68, 750-756. https://doi.org/10.1139/z90-108

Turner RK. 2000. Integrating natural and socio-economic science in coastal management. Journal of Marine Systems 25, 447-460. https://doi.org/10.1016

United Nations Economic and Social Council (UNESCO). 2017. Progress towards the Sustainable Development Goals. Report of the Secretary-General 1-19.

Verachia W, Niven B, Bremer PJ. 2012. The effect of postharvest handling and processing on sea urchin (Evechinus chloroticus) gonad quality. International Journal of Food Science and Technology 12, 2545-2553. https://doi.org

Verachia W, Niven B, Then S, Brennan N, Silcock P, Bremer PJ. 2012. Determination of the similarity between gonads recovered from single sea urchins (Evechinus chloroticus) compared to the similarity of gonads recovered from different urchins. LWT-Food Science and Technology 49, 102-107. https://doi.org/10.1080/00288330.2012.697070


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