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Bioactivity of medicinal plants Mentha arvensis and Peganum harmala extracts against Heterotermes indicola (Wasmann) (Isoptera)

Research Paper | November 1, 2015

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Ayesha Aihetasham, Maryam Umer, Muhammad Saeed Akhtar, Muhammad Imran Din, Khalid Zamir Rasib

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Int. J. Biosci.7( 5), 116-126, November 2015

DOI: http://dx.doi.org/10.12692/ijb/7.5.116-126


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As termites are hazardous causing damages to wooden structures leading to huge economic loss, efforts are directed towards the study of plant based products for effective and eco-friendly termite control. Present study was performed to determine the chemical constituents of the seed extracts from two medicinal plant species of Peganum harmala and Mentha arvensis against Heterotermes indicola. An impregnated filter paper no-choice bioassay method was applied. In Gas Chromatography Mass Spectrometry (GC-MS) analysis of M. arvensis, eleven different compounds were identified. Compounds in seed extractives of P. harmala were Cyclopropanepentanoic acid, 2-undecyl-, methyl ester, trans 5-Octadecenoic acid, methyl ester, Linoleic acid ethyl ester, Leptaflorine and Harmine. The biological activity of these seed extracts was investigated against H. indicola in laboratory bioassay. Results revealed that extracts of P. harmala exhibited anti-termitic activity in a dose-dependent manner and showed a significant activity after 40 hours of exposure while the extract of M. arvensis was toxic and also attractant.TheLT50 value of M. arvensis was 38.48, 67.54 and 83.19 hour respectively for 10%, 5% and 3% respectively. However, In case of P. harmala the LT50 was76.56,142.6 and 147.9 hours for 10%, 5% and 3% respectively. Gas Chromatography Mass Spectrometry (GC-MS) identified components could be further utilizes to explore antitermitic activity of each component against other pest termite species in Pakistan.


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Bioactivity of medicinal plants Mentha arvensis and Peganum harmala extracts against Heterotermes indicola (Wasmann) (Isoptera)

Abbas M, Shahid M, Iqbal M, Anjum F, Sharif S, Ahmed S, Pirzada T. 2013. Antitermitic activity and phytochemical analysis of fifteen medicinal plant seeds. Journal of Medicinal Plant Research7 (22), 1608-1617. http://dx.doi.org/10.5897/JMPR12.683

Al-Alawy, Sady Abd–Al mehsin. 1987. Classification and ecological studies of termites in Iraq, Phd. Thesis, college of agriculture, Baghdad university .323 P. (Arabic)

Ahmed S, Hussain A, Zafar MI, Riaz, MA, Shahid M. 2011. Evaluation of Plant Extracts on Mortality and Tunneling Activities of Subterranean Termites in Pakistan. INTECH Open Access Publisher.

Ajayi OE, Adedire CO, LajideL. 2012. Evaluation of Partially Purified Fractions of Crude Extracts of the Leaves of Morinda lucida (Benth.) and Datura stramonium (L.) for Suppression of Wood Damage by Subterranean Termites. Journal of Agricultural Science 4(5), p125. http://dx.doi.org/10.5539/jas.v4n5p125

Akhtar MS. 1983. Wood destroying termites (Isoptera: Rhinotermitidae) of Pakistan, Material und Organismen 18(4), 277-291.

Brito AM. 2007. Avaliaηγo da atividadeantileishmanial dos σleosessenciais das plantas Cymbopogoncitratus (DC) Stapf, Eucalyptus citriodora Hook, Menthaarvensis L, e MenthapiperitaL. Dissertaηγo do programa de pσs-graduaηγoem Saϊde e Ambienteda Universidade Tiradentes. http://dx.doi.org/10.4103/0975-1483.57066

Benner JP. 1993. Pesticidal compounds from higher plants. Pesticide Science 39, 95-102. http://dx.doi.org/10.1002/ps.2780390202

Bertini LM, Perieira AF, Oliveira CLL, Menezes EA, Morais SM, Cunha FA, Cavalcanti ESB. 2005. Perfi de sensibilidade de bactériasfrente a óleosessenciais de algumasplantas do nordeste do Brasil.-Infarma 17, 80- 83.

Castillo-Juarez I, Gonzalez V, Jaime-Aguilar H, Martinez G, Linares E, Bye R, Romero I. 2009. Anti-Helicobacter pylori activity of plants used in Mexican traditional medicine for gastrointestinal disorders.  Journal  of  Ethnopharmacology 122(2), 402-405. http://dx.doi.org/10.1016/j.jep.2008.12.021

Cheng SS, Chang HT, Wu CL, Chang ST. 2007. Anti-termitic activities of essential oils from coniferous trees against Coptotermes formosanus. BioresourceTechnology 98, 456-459. http://dx.doi.org/10.1016/j.biortech.2006.01.006

Chang ST, Cheng SS, Wang SY. 2001. Antitermitic activity of essential oils and components from Taiwania (Taiwania cryptomerioides). Journal of chemical ecology 27, 717–724.

Delate KM, Grace JK. 1995. Susceptibility of neem to attack by the Formosan Subterranean Termite, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae). Journal of Applied Entomology 119, 93-95. http://dx.doi.org/10.1111/j.1439-0418.1995.tb01250.x

Deschamps C, Zanatta JL, Roswalka L, Oliveira MC, Bizzo HR, Alquini Y. 2006. Densidade de tricomasglandulares e produηγo de σleoessencialemMentha arvensis L, Mentha x piperita L. e Mentha cf. aquatica L. Ciκncia e Natura, UFSM 28, 23-34.

Ding W, Hu XP. 2010. Antitermitic effect of the Lantana camara plant on subterranean termites (Isoptera: Rhinotermitidae). Insect Science 17(5), 427–433. http://dx.doi.org/10.1111/j.1744-7917.2010.01326.x

Elango G, Rahuman AA, Kamaraj C, Bagavan A, Zahir AA, Santhoshkumar T, Rajakumar G. 2012. Efficacy of medicinal plant extracts against Formosan subterranean termite, Coptotermes formosanus. Industrial Crops and Products 36(1), 524-530. http://dx.doi.org/10.1016/j.indcrop.2011.10.032

Finney DJ. 1971. Probit analysis 3rd edition. Cambridge University, London, UK 333.

Gende LB, Mendiara S, Fernandez NJ, Baren CV, Lira AD, Bandoni A, Fritz R, FlorisI. Eguarus M. 2014. Essentials oils of some Mentha spp. and their relation with antimicrobial activity against Paenibacillus larvae, the causative agent of American foulbrood in honey bees, by using the bioautography technique. Bulletin of Insectology 67 (1), 13-20.

Gracindo LAMB, Grisi MCM, Silva DB, Alves RBN, Bizzo HR, Vieira RF. 2006.. Chemical characterization of mint (Mentha spp.) germplasm at Federal District, Brazil. Revista Brasileira de Plantas Medicinais 8, 5-9.

Hertel H. 2000. Finding Alternatives to Persistent Organic Pollutants (POPS) for Termite Management. Global IPM Facility Expert Group Termite Biology Management Stockholm Convention, FAO, Rome, Italy, 118- 168.

Isman MB. 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomology 51, 45-66. http://dx.doi.org/10.1146/annurev.ento.51.110104.151146

Jembere B, Getahun D, Negash M, Sevoum E. 2005. Toxicity of Birbira (Milletia erruginea) seed crude extracts to some insect pests as compared to other botanical and synthetic insecticides. 11th NAPRECA (Natural Products and Drug Delivery) Symposium Book of Proceeding, Astanarivo, Madagascar, 88-96 P.

Lee SE, Lee BH, Choi WS, Park BS, Kim JG, Campbell BC. 2001. Fumigant Toxicity of volatile natural products from Korean spices and medicinal plants towards the rice weevil, Sitophilus oryzae(L) Pest Management Science 57(6), 548-553). http://dx.doi.org/10.1002/ps.322

Lewis VR. 1997. Alternative control strategies for termites. Journal of Agricultural Entomology 14(3), 291–307. http://dx.doi.org/10.1016/j.ibiod.2009.05.009

Logan JWM, Cowie RH, Wood TG. 1990. Termite (Isoptera) control in agriculture and forestry by non-chemical methods: a review. Bulletin of Entomological Research 80(3), 309-330 http://dx.doi.org/10.1017/S0007485300050513

Matias JR. 2005. Menthol propyleneglycol carbonate and analogs there of as insect pest repellents. World intellectual property organization. PCT international publication number WO 2005/025313 A1.

Malaka    SLO.   1996.   Termites   in    West   Africa. 1stedition. University of Lagos press,1-151.

Meepagala KM, Osbrink W, Sturtz G, Lax A. 2006. Plant‐derived natural products exhibiting activity against formosan subterranean termites (Coptotermes formosanus). Pest management science 62(6), 565-570. http://dx.doi.org/10.1002/ps.1214Nasciment

EMM, Rodrigues FFG, Campos AR, Costa JGM. 2009. Phytochemical prospection, toxicity and antimicrobial  activity  of  Menth  arvensis  (labiatae) from northeast of Brazil. Pharmacognosys 1(3), 210-212. http://dx.doi.org/10.4103/0975-1483.57066

Nisar MS, Ahmed S, Ashfaq M, Sahi ST. 2012. Effect of Leaf and Seed Extracts of Jatropha curcas Linn. On Mortality and Tunneling of Subterranean Termites, Odontotermes obesus (Ramb.) (Termitidae: Isoptera).Pakistan Journal of Life and Social Sciences 10(1), 33-38.

Ohtani Y, Hazama M, Sameshima K. 1996. Crucial chemical factors of the termiticidal activity of hinoki wood (Chamaecyparis obtusa) II. Variations in termiticidal activities among five individual samples of hinoki wood. Mokuzai Gakkaishi 42, 1228-1233.

Oliver B. 1960. Nigeria’s useful plants. Nigerian Field, 25(4), 174-192.

Park IK, Shin SC. 2005. Fumigant activity of plant essential oils and components from garlic (Allium sativum) and clove bud (Eugenia caryophyllata) oils against the Japanese termite (Reticulitermes speratus Kolbe). Journal of Agriculture, Food and Chemistry 53, 4388-92. http://dx.doi.org/10.1021/jf050393r

Pramila DM, Xavier R, Marimuthu K, Kathiresan S, Khoo ML, Senthilkumar M, Sreeramanan S. 2012. Phytochemical analysis and antimicrobial potential of methanolic leaf extract of peppermint (Menthapiperita: Lamiaceae). Jounal of Medicinal Plants Research 6(2), 331-335. http://dx.doi.org/10.5897/JMPR11.1232

Qureshi NA, Qureshi MZ, Ali N, Athar M, Aziz Ullah A. 2012. Protozoidal activities of Eucalyptus cammeldulensis, Dalbergia sissoo and Acacia arabica woods and their different parts on the entozoic flagellates of Heterotermes indicola and Coptotermes heimi. African Journal of Biotechnology 11(57), 12094-12102. http://dx.doi.org/10.5897/AJB12.375

Qureshi NA, Ashraf A, Afzal M, Naseer Ullah IA, Haleem S. 2015. Toxic potential of Melia azedarach leaves extract against Odontotermes obesus and Microtermes obesi. International Journal of Biosciences l6(2), 120-127. http://dx.doi.org/10.12692/ijb/6.2.120-127

Sakasegawa M, Hori K, Yatagi M. 2003. Composition and anti-termite activities of essential oils and Melaleuca species. Journal of Wood Science 49(2), 181-187. http://dx.doi.org/10.1007/s100860300029

Sartoratto A, Machado ALM, Delarmilina C, Figueira GM, Duarte MCT, Rehder VL. 2004.Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil. Brazilian journal of microbiology 35, 275-280. http://dx.doi.org/10.1590/S1517838220040003000 01

Sattar A, Naeem M, Ehsan-ul-Haq. 2014. Efficacy of Plant Extracts Against Subterranean Termites i.e. Microtermes obesi and Odontotermes lokanandi (Blattodea:Termitidae). Journal of Biodiversity Bioprospecting and Development 1,122. http://dx.doi.org/10.4172/ijbbd.1000122

Shonouda  M, Osman  S, Salama  O, Ayoub  A. 2008. Toxical effect of Peganum harmala L. leaves on the cotton leaf worm, Spodoptera littoralis Boisd and its parasitoids Microplitis rufiventris Kok. Pakistan Journal of Biological Sciences 11(4), 546-52. http://dx.doi.org/10.3923/pjbs.2008.546.552

Supriadi Ismanto A. 2010. Potential Use of Botanical Termiticide. Perspektif, 9(1), 12-20.

Thamer SJ. 2008. The effect of some plants extracts and essential oils on the worker of termite laboratory Microcerotermes gabriles (Isoptera: Termitidae).Bas.J.Vet.,Res. 7 (2).

Venkateswara Rao J, Parvathi K, Kavitha P, Jakka NM, Pallela R. 2005. Effect of chlorpyrifos and monocrotophos on locomotor behavior and acetylcholinesterase activity of subterranean termites, Odontotermes obesus. Pest Management Science 61, 417-421. http://dx.doi.org/10.1002/ps.986Verma

M, Sharma S, Prasad R.2009. Biological alternatives for termite control: a review. International Journal of Biodeterioration & Biodegradation 63(8), 959-972. http://dx.doi.org/10.1016/j.ibiod.2009.05.00.9

Zhu BCR, Henderson G, Chen F, Fei H, Laine RA. 2001. Evaluation of vetiver oil and seven insect active essential oils against the Formosan subterranean termite. Journal of Chemical Ecology 27(8), 1617-25. http://dx.doi.org/10.1023/a:1010410325174