Biochemical and stability analysis of variations of indigenous Brassica juncea genotypes in different agro-climatic environmental conditions of Pakistan

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Research Paper 01/06/2018
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Biochemical and stability analysis of variations of indigenous Brassica juncea genotypes in different agro-climatic environmental conditions of Pakistan

Faiza Nawaz, Hakim Khan, Naushad Ali Turi, Inamullah, Sidra Pervez, Sadia Qayum, Farrah Andaleeb, Saira Gul, Farhana Maqbool, Ayesha Bibi
Int. J. Biosci.12( 6), 139-151, June 2018.
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Stability and biochemical analysis give the information about the genetic diversity of plants, which further help the scientists and breeders for the cultivation of plants in different climatic conditions. The local indigenous species collection from the different environments are the best for researcher because these local varieties have many resistant genes against environmental disasters. Reproducible and diverse genotypes are very important for the successful plant breeding. Therefore, this study was designed to analyse the stability and biochemical profiling of different locally collected genotypes of Brassica juncea. For this purpose, 25 genotypes of B. juncea along with one check cultivar were obtained from various regions of Pakistan. Stability was investigated using Randomized Complete Block Design (RCBD) and ANOVA while biochemical analysis was performed using Near Infra-Red (NIR) spectroscopy. ANOVA results showed highly significant differences in the all studied parameters for genotypes, genotypes x year, location x year, genotypes x location x year except location which was no significant with an overall (cv) coefficient of variance was 3.37 for days to flower completion, 7.05 for seed silique, 4.25 for protein content, 5.06 for glucosinolates and 4.57 for erucic acid. Stability analysis showed that 1611, 1616 and 1617 were the most stable varieties in adverse environmental condition because they have less b regression coefficient and S2d deviation from regression. Current findings revealed that among all checked genotypes, some remained stable under different harsh environmental conditions for abiotic factors further no effect of these adverse conditions was observed on biochemical profiling.


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