Biochemical characterization of Pakistani Zea mays landraces growing in the remote areas of Khyber Pakhtunkhwa

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Research Paper 01/09/2017
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Biochemical characterization of Pakistani Zea mays landraces growing in the remote areas of Khyber Pakhtunkhwa

Murad Ali, Marwa Alam, Syed Fazal Wadood, Waqar Khan, Nisar Uddin, Shams Uz Zaman, Nausheen Nazir, Nasiara Karim, Zahid Ali, Mohammad Nisar
Int. J. Biosci.11( 3), 51-58, September 2017.
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Abstract

The landraces extensity cultivated in Malak and Division of Pakistan hold important adaptations to local environmental condition, being express significant level of genetic diversity. In the present investigation, The Genetic divergence in 29 zea maize landraces was assessed using seed storage protein through SDS-PAGE. In total, eight loci were express which were entirely polymorphic and high level of genetic diversity was observed in L-8 i.e.10.34% followed by L-2 with 17.2% polymorphism. Among landraces, ST-2 was unique because of the expression of all loci. Genetic tree based on protein binary data matrix delineated landraces into two main groups. It was found that high level of divergence exist within and between collection sites. In dendrogram, each group represented landraces of different collection sites, but group-1 was the diverse group and represented high level of genetic diversity within landraces reported from Swat, Bajaur, Dir and Buner.In Group-2 majority of the landraces showing tight linkage, although originated from different agro ecological zones like Malakand, Buner and Dir. This may be due to the utilization of shared gene pool at different agro ecological zones. Significant level of genetic diversity existed within landraces will decrease the genetic vulnerability and will help in the selection of superior genotypes for future breeding programs.

VIEWS 4

Lopes MA, Arkins BA.1996. Molecular biology and traditional breeding applied to the improvement of maize nutritional quality In: SOBRAL, B.W.S, (ed).The impact of plant molecular genetics. Springer, the Netherlands.

Gerpacio VR, Pangali PL. 2007. Tropical and subtropical maize in Asia: Production system constraints and Research Priorities” CIMMYT Mexico, ISBN: 978-970-648-155-9, 935 P.

Chaudhry FM. 1994.Kharif Cereal Crops. In: (Eds.): E. Bashir and R. Bantel. Crop Production, National Book Foundation, Islamabad, Pakistan.

Akbar M, Shakoor MS, Hussain A, Sarwar M. 2008. Evaluation of maize 3 way crosses through genetic variability, broad sense heritability, characters association and path analysis. Journal of agricultural resources. 46(1), 39-45.

Abdullah KK. 1999. Corn (maize) response to different methods of nitrogen fertilizer placement periodically at Quetta. Sarhad Journal of Agriculture, 15, 164.

Bellon MR. 2004. Conceptualizing interventions to support on-farm genetic resource conservation. World Development 32(1), 159-172.

GhafoorA, Ahmad Z, Afzal M. 2005. Use of SDS-PAGE markers for determining quantitative traits loci in blackgram  [Vignamungo (L.) Hepper] germplasm. Pakistan Journal of Botany, 37(2), 263-269.

NisarM, Ghafoor A, Ahmad H, Khan MR, Qureshi AS, Ali H, Aslam M. 2008. Evaluation of genetic diversity of pea germplasm through phenotypic trait analysis. Pakistan Journal of Botany, 40, 2081-2086.

Ghafoor A, Ahmad Z, Afzal M. 2005. Use of SDS-PAGE markers for determining quantitative traits loci in blackgram  [Vignamungo (L.) Hepper] germplasm. Pakistan Journal of Botany, 37(2), 263-269.

Wadood SF, Hassan N, Khaliq A, Nausheen Jan T, Ghafoor A, Khan M, Nisar M. 2016. Genetic polymorphism in Lens culinaris collected from Malakand division Khayber Pakhtunkhwa, Pakistan. Journal of Biodiversity and Environmental Sciences, 8(2), 53-60.

Kozioł MJ. 1992. Chemical composition and nutritional evaluation of quinoa (Chenopodium quinoa Willd.). Journal of Food Composition and Analysis, 5(1), 35-68.PCP.