Biocontrol of larvae of dengue vector Aedes aegypti (L.) Using fresh seed extract of some selected indigenous plants

Paper Details

Research Paper 01/10/2020
Views (291) Download (19)

Biocontrol of larvae of dengue vector Aedes aegypti (L.) Using fresh seed extract of some selected indigenous plants

Md. Ahsan Shahriar Tohfa, Tahmina Akter, Md. Junayed, Saadia Ahmad
Int. J. Biosci.17( 4), 46-59, October 2020.
Certificate: IJB 2020 [Generate Certificate]


Present study was conducted to control dengue vector mosquito, Aedes aegypti using environmentally safe biological materials, the seeds of some selected indigenous plants under laboratory condition. Here test plants were Anacardium occidentale, Azadirachta indica, Corchorus capsularis, Momordica charantia, Swietenia mahagoni and Terminalia catappa. For this late 3rd instar larvae of Aedes aegypti (L.) were exposed to different concentrations (5%, 4%, 3%, 2% and 1%) of aquatic extract of fresh seeds of 6 selected plants. After 24 hours of exposure highest mortality (95%) observed in crude seed extract of Corchorus capsularis followed by 90% mortality in crude extract of Anacardium occidentale and Swietenia mahagoni, 75% in Azadirachta indica, 60% in Terminalia catappa respectively. Lowest mortality observed in Momordica charantia killing 50% larvae after 24 hours of exposure. Among all the test plants minimum LC50 value was recorded in fresh seed extract of Corchorus capsularis (1.020) followed by Anacardium occidentale (1.44), Azadiracta indica (2.44), Swietenia mahagoni (3.11), Terminalia catappa (3.78) and Momordica charantia (5.47) after 24 hours of exposure. Among all the test plants crude seed extract of Corchorus capsularis was observed most toxic against late 3rd instar larvae of Aedes aegypti having LC50 (1.02, 0.78 and 0.59), Lc90 (4.74, 3.30 and 2.27) and LC95 (5.96, 4.47 and 3.32) values after 24, 48 and 72 hours of exposure respectively. From the above study, it can be said that plant seeds might contain certain phytochemicals that can be used in controlling Aedes mosquitoe larvae is an ideal environmental healthy approach.


Ahmed SM, Chandler H, Peroira J. 1981. Insecticidal potential and biological activity of Indian indigenous plants against Muscadomestica International Pest Control 23, 170-175.

Burdick GE, Arris EJ, Dean HJ, Walker TM, Skea J, Colby D. 1964. Transaction of the American Fisheries Society 93, 127-136.[127:TAODIL]2.0.CO;2

Chowdhury MA, Hossain MI, Ahmed TU. 2002. Aedes larval survey report in Dhaka. Star Weekend Magazine. August   23, 2002.

Cottam L. 1965. The ecologists’ role in problem of pesticide pollution. Bioscience 15, 457-463.

Croft BA, Brown AWA. 1975. Responses of arthropod natural enemies to insecticides. Annual Review of Entomology 20, 285- 335.

Das NG, Goswami D, Rabha B. 2007. Preliminary evolution of mosquito larvicidal efficacy of plant extracts. Journal Veterinary Borne Disease 44, 145-148.

Desmarchelier JM. 1979. Stability and efficacy of pyrethrins on grain in storage. Pyrethrum   Post 15, 3-8.

Dinham   B. 1993. The pesticide hazard: a global health and environmental audit. Zed Books, London p 283.

Elangovan A, Dhanasekaran S, Anandan A, Krishnappa K, Gokulakrishnan J, Elumalai K. 2012. Mosquitocidal activities of Corchorus capsularis L (Malvaceae) against a common malaria vector, Anopheles stephensi (Liston) and a dengue vector Aedes aegypti (L) (Diptera: Culicidae). International Journal of Recent Scientific Research 3(6), 564-8.

Freedmen B, Nowak LJ, Kwolek WF, Berry EC, Guthrie WD. 1979. A bioassay for plant-derived pest control agents using the European corn borer. Journal of Economic Entomology 72, 541-545.

Gerrits R, Van Latum EBJ. 1988. Plant derived pesticides in developing countries- possibilities and research needs. Ministry of Housing, Physical Planning and Environment, Hague, the Netherlands p 104.

Grainge M, Ahmed S. 1988. Handbook of Plants with Pest-control properties. John Wiley and Sons, New York p 470.

Gubler DJ, Kuno G. 1997. Dengue and Dengue Hemorrhagic Fever. CAB International. Cambridge.

Horsfall WR. 1955. Mosquitoes, Their Bionomics and Relation to Disease. The Ronald Press Company, New York p 409-532.

Heckman CW. 1993. The fate of pesticides in heavily polluted aquatic systems. In: Hypertrophic and polluted freshwater ecosystems: Ecological bases for water resource management p 39-90.

Hossain MI, Ameen M, Rafique AKM. 1995. Efficacy of two pyrethroid insecticidal against Culex quinquefasciatus larvae in Dhaka city. Bangladesh Journal of Zoology 23, 187-192.

Jalees S, Sharma SK, Rahman SJ, Verghese T. 1993. Evaluation of   insecticidal properties of an indigenous plant, Cannabis sativa (Linn.), against mosquito larvae under laboratory conditions. Journal of Entomological Research (New Delhi) 17(2), 117-120.

Kalra RL, Chawla RP. 1986. Pesticidal contamination of foods in the year 2000 188-204. Indian National Science Academy, New Delhi.

Koul O. 1982. Insect feeding deterrents in plants. Indian Review of Life Science 2, 97-125.

Koneri R, Pontororing HH. 2016. Uji Ekstrak Biji Mahoni (Swietenia macrophylla) terhadap Larva Aedes aegypti Vektor Penyakit Demam Berdarah. Media Kesehatan Masyarakat Indonesia. The Indonesian Journal of Public Health 12(4), 216-223.

Khan JK. 1999. Effect of the crude extract of different plant on the larvae of mosquito Jahangirnagar University, Bangladesh.iii+69., Culex quinquefasciatus Say (Diptera: Culicidae). M. Sc. Thesis, Department of Zoology. Jahangirnagar University, Bangladesh iii+69.

Marini- Bettello GB. 1997. Modern trends in the use of natural products for controlling pests and plant diseases. In: Marini-Bettello, G.B. (ed.).Natural products and protection of plants. Elsevier Scientific Publishing Company, New York. p 5-13.

Metcalf CL. 1980. Changing role of insecticides in crop protection. Annual Review of Entomology 25, 219- 256.

Monzon RB, Alvior JP, Luczon LC, Morales AS, Mutuc F. 1994. Larvicidal potential of five Philippines plants against Aedes aegypti and Culex quinquefasciatus Southeast Asian Journal of Tropical medicine Public health 23, 755-759.

Moses M. 1991. Pesticide related health problems and farmworkers. In: The pesticide   handbook profiles for action, 3rd edition pp. 283- 295.

Mulrennan JJA. 1995. Vector control without chemicals: A public health perspective. Journal of American Mosquito Control Association 11, 256-257.

Minjas JN, Sarda RK. 1986. Laboratory observations of the toxicity of Swartziam madagascariensis extract to mosquito larvae. Transitional Royal Society of Tropical Medicine 80, 460-461.

Nour AH, Jessinta D, Nour AH. 2012. Larvicidal activity of extracts from different parts of neem (Azadirachta indica) against Aedes aegypti mosquitos’ larvae. Scientific Research and essays 7(31), 2810-2815.

Pimental D, Acquay H, Biltonen M, Rice P, Silva M, Nelson J, Lipner V, Giordano S, Horowitz A, Amoer M. 1992. Environmental and economic costs of pesticide use. Bioscience 42, 750- 760.

Prain D. 1963. Bangal plants Botanical survey of India. Calcutta, p 291.

Scot IM, Kaushik NK. 1998. The toxicity of Margosan, a product of neem.

Singh RK, Dhiman RC, Mittal PK. 2006. Mosquito larvicidal properties of Momordica charantia Linn. (Family: Cucarbitaceae). Parasitology Research 102(5), 45-47.

Weekly Epidemiological Record. 2002. International Conference on Mosquito Control, Controlling Aedes aegypti (vector of Dengue and Yellow Fever) and general mosquito control. Dengue Bulletin   24, 137-138.