Biodegradation of polystyrene by bacteria isolated from talabang tsinelas (Crassostrea iredalei) in Buguey Lagoon, Cagayan
Paper Details
Biodegradation of polystyrene by bacteria isolated from talabang tsinelas (Crassostrea iredalei) in Buguey Lagoon, Cagayan
Abstract
The substantial surge in waste entering our aquatic ecosystems poses one of the leading environmental hazards. With the Philippines ranked as the third-largest contributor of plastic into the ocean annually, efforts to mitigate this issue through biodegradable alternatives are crucial. This study assessed the polystyrene (PS) biodegradation capability of microorganisms from talabang tsinelas (Crassostrea iredalei) in Buguey Lagoon, Cagayan. Two distinct colonies, Pseudomonas aeruginosa and Enterobacter cloacae, were identified using the API 20E biochemical test kit for their potential in polymer degradation. SEM analysis confirmed substantial surface deterioration of PS films exposed to these strains, indicating microbial degradation. One-way ANOVA analysis revealed no significant difference in polystyrene degradation among the isolated microorganisms, as all p-values exceeded the 0.05 significance level. The F-value for days 15, 30, and 45 were 1.207892, 1.324312, 1.665196, 1.665196, respectively, with corresponding p-values of 0.362385 for day zero (0), 0.333897 for day fifteen (15), 0.265922 on day thirty (30), and 0.167041 for day forty-five (45). These results indicate that the weight loss of polystyrene over time did not significantly differ between P. aeruginosa and E. cloacae. Results of the SEM also revealed the ability of P. aeruginosa and E. cloacae, to degrade PS plastic. The PS film subjected to P. aeruginosa exhibited the most extensive surface deterioration. This was characterized by the formation of deep pits, significant increases in surface roughness, and the development of cracks. Conversely, the PS film exposed to E. cloacae exhibited minimal or lesser degree of surface degradation compared to P. aeruginosa.
Asmita K, Shubhamsingh T, Tejashree S, Road DW, Road DW. 2015. Isolation of plastic degrading micro-organisms from soil samples collected at various locations in Mumbai, India. International Research Journal of Environment Sciences 4(3), 77–85.
Balasubramanian V, Natarajan K, Hemambika B, Ramesh N, Sumathi CS, Kottaimuthu R, Rajesh Kannan V. 2010. High-density polyethylene (HDPE)-degrading potential bacteria from marine ecosystem of Gulf of Mannar, India. Letters in Applied Microbiology 51(2), 205-211. https://doi.org/10.1111/j.1472-765X.2010.02883.x
Bhatia M, Girdhar A, Tiwari A, Nayarisseri A. 2014. Implications of a novel Pseudomonas species on low-density polyethylene biodegradation: an in vitro to in silico approach. SpringerPlus 3, 497. https://doi.org/10.1186/2193-1801-3-497
Das MP, Kumar S. 2015. An approach to low-density polyethylene biodegradation by Bacillus amyloliquefaciens. 3 Biotech 5(1), 81-86. https://doi.org/10.1007/s13205-014-0205-1
Domingo LC. 2016. Oyster industry expats on Cagayan coast. The Manila Times, p. B5. http://hdl.handle.net/20.500.12174/1628
Fisheries and Aquaculture Department, Food and Agriculture Organization of the United Nations (FAO). 2018. The state of world fisheries and aquaculture: meeting the sustainable development goals. Food and Agriculture Organization of the United Nations. https://doi.org/10.1364/OE.17.003331
Ghosh SK, Pal S, Ray S. 2013. Study of microbes having potentiality for biodegradation of plastics. Environmental Science and Pollution Research International 20(7), 4339-4355. https://doi.org/10.1007/s11356-013-1706-x
Gilan I, Hadar Y, Sivan A. 2004. Colonization, biofilm formation, and biodegradation of polyethylene by a strain of Rhodococcus ruber. Applied Microbiology and Biotechnology 65(1), 97-104. https://doi.org/10.1007/s00253-004-1584-8
Jambre KGE. 2021. Density of microplastics in Philippine cupped oyster (Crassostrea iredalei). International Journal of Advanced Multidisciplinary Studies 1(4), 287-294.
Jiang J, Wu J. 2012. Isolation and characterization of polycyclic aromatic hydrocarbons-degrading Enterobacter sp. strain GS-1. Microbiological Research 167(3), 151-156.
Joshi G, Goswami P, Verma P, Prakash G, Simon P, Vinithkumar NV, Dharani G. 2022. Unraveling the plastic degradation potentials of the plastisphere-associated marine bacterial consortium as a key player for the low-density polyethylene degradation. Journal of Hazardous Materials 425, 128005. https://doi.org/10.1016/j.jhazmat.2021.128005
Kyaw BM, Champakalakshmi R, Sakharkar MK, Lim CS, Sakharkar KR. 2012. Biodegradation of low-density polythene (LDPE) by Pseudomonas species. Indian Journal of Microbiology 52(3), 411-419. https://doi.org/10.1007/s12088-012-0250-6
Luis-Villaseñor IE, Zamudio-Armenta OO, Voltolina D, Rochin-Arenas JA, Gómez-Gil B, Audelo-Naranjo JM, Flores-Higuera FA. 2018. Bacterial communities of the oysters Crassostrea corteziensis and C. sikamea of Cospita Bay, Sinaloa, Mexico. Revista Internacional de Contaminación Ambiental 34(2), 203-213. https://doi.org/10.20937/RICA.2018.34.02.02
Mohanan N, Montazer Z, Sharma PK, Levin DB. 2020. Microbial and enzymatic degradation of synthetic plastics. Frontiers in Microbiology 11, 580709. https://doi.org/10.3389/fmicb.2020.580709
Moradali MF, Ghods S, Rehm BH. 2017. Pseudomonas aeruginosa lifestyle: a paradigm for adaptation, survival, and persistence. Frontiers in Cellular and Infection Microbiology 7, 39. https://doi.org/10.3389/fcimb.2017.00039
Pathak VM, Navneet. 2017. Review on the current status of polymer degradation: a microbial approach. Bioresource and Bioprocessing 4, 15. https://doi.org/10.1186/s40643-017-0145-9
Prakash S, Vadivel V. 2014. Biodegradation of plastics by Pseudomonas putida and Enterobacter cloacae isolated from mangrove soil. Journal of Pure and Applied Microbiology 8(6), 4703-4708.
Pramila R, Ramesh KV. 2011. Biodegradation of low-density polyethylene (LDPE) by fungi isolated from marine water: a SEM analysis. African Journal of Microbiology Research 5(28), 5013-5018. https://doi.org/10.5897/AJMR11.670
Sarker RK, Chakraborty P, Paul P, Chatterjee A, Tribedi P. 2020. Degradation of low-density polyethylene (LDPE) by Enterobacter cloacae AKS7: a potential step towards sustainable environmental remediation. Archives of Microbiology 202(8), 2117-2125. https://doi.org/10.1007/s00203-020-01926-8
Wei R, Wierckx N. 2021. Editorial: microbial degradation of plastics. Frontiers in Microbiology 12, 635621. https://doi.org/10.3389/fmicb.2021.635621
Yang Y, Wang J, Xia M. 2020. Biodegradation and mineralization of polystyrene by plastic-eating superworms Zophobas atratus. Science of the Total Environment 708, 135233. https://doi.org/10.1016/j.scitotenv.2019.135233
Yang Y, Yang J, Wu WM, Zhao J, Song Y, Gao L, Yang R, Jiang L. 2015. Biodegradation and mineralization of polystyrene by plastic-eating mealworms: part 2. role of gut microorganisms. Environmental Science & Technology 49(20), 12087-12093. https://doi.org/10.1021/acs.est.5b02663
Robelyn N. Marcos, Jen Pearl L. Benarao, Danica Marie T. Pagatpatan, Mark Key John V. Sabio, Madel B. Tanguilan, Jefferson K. Soriano (2025), Biodegradation of polystyrene by bacteria isolated from talabang tsinelas (Crassostrea iredalei) in Buguey Lagoon, Cagayan; IJMM, V26, N3, March, P1-11
https://innspub.net/biodegradation-of-polystyrene-by-bacteria-isolated-from-talabang-tsinelas-crassostrea-iredalei-in-buguey-lagoon-cagayan/
Copyright © 2025
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0