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Research Paper | April 1, 2015

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Biodegradation performance of sequencing batch biofilm reactor for Polychlorinated biphenyls in transformer oil

Fahimeh Teimouri, Mahnaz Nikaeen, Mohammad Mehdi Amin

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J. Bio. Env. Sci.6(4), 65-74, April 2015

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Abstract

In order that the biodegradability of oil contain PCBs (aroclor 1242 and 1254) from electrical transformer, two pilot – scale sequencing batch biofilm reactor were used with polyurethane foam as an inert and growth media. The reactors were operated for 238 days. During the operation, the effects of main operation parameters such as pH, organic loading rate, PCBs loading rate, co-substrate type and initial COD concentration were investigated. It was observed that microorganisms were able to utilize and degrade the oil containing PCBs. Optical density results showed that the increase of neutral pH resulted in the increase in cell number and optical density. The reactors showed PCBs removal efficiency more than 99%, with effluent PCBs concentration of lower than 3µg/l. COD removal efficiency and surface loading rate increased as the initial concentration of PCBs increased. The average of COD reduction by SBBRs was more than 85% corresponding to COD <100 mg/l in reactors effluent. The average of SLR in two reactors was more than 4 corresponding to PCBs removal efficiency which was more than 99% in two reactors. GC-MS chromatography analyses detected the chlorobenzoic compounds in reactors effluent as the byproduct of PCBs biodegradation. The results of 16s rDNA sequence analysis showed that Rodococcus, Pseudoxanthomonas, Agromycess and Pseudomonas were predominant bacteria in reactors. The study showed that SBBRs have a good potential for biodegradation of oil contain PCBs. Also, acetic acid could be recommended as a good co-substrate and alternative solvent because it is inexpensive, abundant and safe for environment.

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Biodegradation performance of sequencing batch biofilm reactor for Polychlorinated biphenyls in transformer oil

Abraham WR, Nogales B, Golyshin PN, Pieper DH, Timmis KN. 2002. Polychlorinated biphenyl-degrading microbial communities in soils and sediments. Current Opinion in Microbiology 5, 246-253.

Amin M. 2004. Performance evaluation of three anaerobic bioreactors, ASBR, HAIS, and UASB. PhD Thesis.Department of Civil and Environmental Engineering University of Illinois at Urbana-Champaign (UIUC) Illinois, USA.

Anthony F. 2000. Bioremediation of polychlorinated biphenyl pollutants with butane-utilizing bacteria, US Patent 6,156,203.

APHA. 2012. Standard Methods for the Examination of Water and Wastewater. American Public Health Association, American Water Works Association, Water Environment Federation.

Atlas RM, PHILP J. 2005. Bioremediation: Applied microbial solutions for real-world environmental cleanup, ASM Press.

ATSDR. 2001. Polychlorinated Biphenyl, U.S. Department of health and human services on line at: http://www.atsdr.cdc.gov/toxfaq.html.

Barrett JC, Fry B, Maller J, Daly M. 2005. Haploview: analysis and visualization of LD and haplotype maps, Bioinformatics 21, 263-265.

Bedard DL, Unterman R, Bopp LH, Brennan MJ, Haberl ML, Johson C. 1986. Rapid assay for screening and characterizing microorganisms for the ability to degrade polychlorinated biphenyls. Applied and environmental microbiology 51, 761-768.

Begonja kolar A, Hrsak D, Fingler S, Ćetkovic H, Petric I, Kolic NU. 2007. PCB-degrading potential of aerobic bacteria enriched from marine sediments. International biodeterioration & biodegradation 60, 16-24.

Borja J, Taleon DM, Auresenia J, Gallardo S. 2005. Polychlorinated biphenyls and their biodegradation. Process Biochemistry 40, 1999-2013.

Casserly C, Erijman L. 2003. Molecular monitoring of microbial diversity in an UASB reactor. International biodeterioration & biodegradation 52, 7-12.

Cheng JZ, Harada R, Campell S, Li Q. 2007. Biodegradation of Dinitrotoluene by Pseudoxanthomonas sp. JA40. J Young Investigators 16(3).

D’angelo E, Nunez A. 2010. Effect of environmental conditions on polychlorinated biphenyl transformations and bacterial communities in a river sediment. Journal of Soils and Sediments 10, 1186-1199.

Dadong S, Changlun C, Xiangke W, Masaaki N. 2010. Removal of polychlorinated biphenyls from aqueous solutions using b-cyclodextrin grafted multiwalled carbon nanotubes. Chemosphere 79, 679.

Fava F, Zanaroli G, Young L. 2003. Microbial reductive dechlorination of pre‐existing PCBs and spiked 2, 3, 4, 5, 6‐pentachlorobiphenyl in anaerobic slurries of a contaminated sediment of Venice Lagoon (Italy). FEMS microbiology ecology 44, 309-318.

Fedi S, Carnevali M, Fava F, Andracchio A, Zappoli S, Zannoni D. 2001. Polychlorinated biphenyl degradation activities and hybridization analyses of fifteen aerobic strains isolated from a PCB-contaminated site. Research in microbiology 152, 583-592.

Gopinath M, Archana K, Saranya G, Priscilla Pushparani V, Dhanasekar R. 2013. Bidegradation of toluene using different bioreactors, a review. National conference on industrial pollution and control technology. International journal of CHEM TECH research 5,1401-1412.

Kuo CE, Liu SM, Liu C. 1999. Biodegradation of coplanar polychlorinated biphenyls by anaerobic microorganisms from estuarine sediments. Chemosphere 39, 1445-1458.

Liu WT, Chan OC, Fang HH. 2002. Characterization of microbial community in granular sludge treating brewery wastewater. Water research 36, 1767-1775.

Mondello F. 2002. Microbial Bioremediation of Polychlorinated Biphenyls: Applicability to the Former GE Canada Transformer Manufacturing Facility Located in Guelph, Ontario, General Electric Company’, GE Global Research Technical Information Series. Online at: www.clu-in.info/…/tinsone.cfm?…%22bioremediation%22% 20and%20…‎

Movahedyan H, Khorsandi H, Salehi R, Nikaeen M. 2009. Detection of phenol degrading bacteria and pseudomonas putida in activated sludge by polymerase chain reaction. Iranian Journal of Environmental Health Science & Engineering 6.

Movahedyan H. 2009. Investigation of Sequencing Bach Reactor efficiency in treatment of wastewater containing 4-ChloroPhenol. PhD thesis. Department of Environmental Engineering, Isfahan University of Medical Sciences School of Health. 71-78.

Okerentugba PO and ezeronye OU. 2003. Petroleum degrading potentials of single and mixed microbial cultures isolated from rivers and refinery effluent in Nigeria. Aferican journal of biotechnology 2,288 -292.

Rittman BE, McCarty PL. 2001. Environmental biotechnology. McGraw-Hill New York.

Sakai M, Masai E, Asami H, Sugiyama K, Kimbara K, Fukuda M. 2002. Diversity of 2, 3-dihydroxybiphenyl dioxygenase genes in a strong PCB degrader,< i> Rhodococcus</i> sp. strain RHA1. Journal of bioscience and bioengineering 93, 421-427.

Seto M, Kimbara K, Shimura M, Hatta T, Fukuda M,Yano K. 1995. A novel transformation of polychlorinated biphenyls by Rhodococcus sp. Strain RHA1. Applied and environmental microbiology 61, 3353-3358.

Tartakovsky B, Michotte A, Cadieux J, Lau P, Hawari J, Guiot S. 2001. Degradation of Aroclor 1242 in a single-stage coupled anaerobic/aerobic bioreactor. Water research 35, 4323-4330.

Torella D, Ellison GM, Mendez-Ferrer S, Ibanez B, Nadal-Ginard B. 2006. Resident human cardiac stem cells: role in cardiac cellular homeostasis and potential for myocardial regeneration. Nature clinical practice Cardiovascular medicine 3, S8-S13.

Wagner-Döbler I, Bennasar A, Vancanneyt M, Strömpl C, Brummer I, Eichner C, Grammel I, Moore E. 1998. Microcosm enrichment of biphenyl-degrading microbial communities from soils and sediments. Applied and environmental microbiology 64, 3014-3022.

Wiegel J, Wu Q. 2000. Microbial reductive dehalogenation of polychlorinated biphenyls. FEMS microbiology ecology 32, 1-15.

Zhao X, Wang Y, Ye Z, Borthwick AG, Ni J. 2006. Oil field wastewater treatment in biological aerated filter by immobilized microorganisms. Process Biochemistry 41, 1475-1483.

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