Biodiesel production from microbial whole cell biocatalyst

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Research Paper 01/08/2013
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Biodiesel production from microbial whole cell biocatalyst

Ravichandran Indumathi, Samuel Paul Raj
J. Biodiv. & Environ. Sci. 3(8), 94-101, August 2013.
Copyright Statement: Copyright 2013; The Author(s).
License: CC BY-NC 4.0

Abstract

Enzymatic mode of biodiesel production has recently gained a special attention among researchers because it eliminates the disadvantages of alkaline process such as end product recovery, waste water treatment, energy intensive and etc. Recently, considerable attention has paid in the direct use of intracellular lipase as a whole-cell biocatalyst (i.e immobilization of the enzyme secreting microbial cells) for biodiesel production where immobilization was carried out at the of microbial cell cultivation. The present investigation is focused on to establish appropriate conditions for immobilized whole cell biocatalyst for the production of biodiesel through transesterification. Fungi strains were isolated from wastes generated from oil industries and screened for the production of extracellular lipolytic enzymes and the most productive strain B2 was identified as Aspergillus sp. for further applications.

Akoh CC, Chang S, Lee G, Shaw J. 2007. Enzymatic approach to biodiesel production. Journal of Agricultural and Food Chemistry 55, 8995-9005.

Ban K, Kaieda M, Matsumoto T, Kondo A, Fukuda H. 2001. Whole-cell biocatalyst for biodiesel fuel production utilizing Rhizopus oryzae cells immobilized within biomass support particles. Biochemical Engineering Journal 8, 39–43.

Fukuda H, Hama S, Tamalampudi S, Noda H. 2008. Wholecell biocatalysts for biodiesel fuel production. Trends in Biotechnology 26, 668–673.

Fukuda H, Kondo A, Tamalampudi S. 2009. Bioenergy: sustainable fuels from biomass by yeast and fungal whole-cell biocatalysts. Biochemical Engineering Journal 44, 2–12.

Kuhad RC, Kapoor M, Rustagi R. 2004. Enhanced production of an alkaline pectinase by Streptomyces sp. RCK-SC by wholecell immobilization and solid-state cultivation. World Journal of Microbiology and Biotechnology 20, 257-263.

Li W, Du W, Liu D. 2007. Rhizopus oryzae IFO 4697 whole cell catalyzed methanolysis of crude and acidified rapeseed oils for biodiesel production in tert-butanol system. Process Biochemistry 42, 1481– 1485.

Meher LC, Vidya SD, Naik SN. 2006. Technical aspects of biodiesel production by transesterification–a review. Renewable and Sustainable Energy Reviews 10, 248–268.

Satyanarayana M, Muraleedharan C. 2011. Comparative Studies of Biodiesel Production from Rubber Seed Oil, Coconut Oil, and Palm Oil Including Thermogravimetric Analysis. Energy Sources Part A 33, 925-937.

Singh AP, Thompson JC, He BB. 2004. A Continuous-flow Reactive Distillation Reactor for Biodiesel Preparation from Seed Oils, ASAE/CSAE Annual International Meeting, Paper number: 046071.

Tamalampudi S, Talukder MR, Hama S, Numata T, Kondo A, Fukuda H. 2008. Enzymatic production of biodiesel from jatropha oil: a comparative study of immobilized-whole cell and commercial lipases as a biocatalyst. Biochemical Engineering Journal 39, 185–189.

Van Gerpen J, Shanks B, Pruszko R, Clements D, Knothe G. 2004. Biodiesel production technology, National Renewable Energy Laboratory NREL/SR-510-36244, Golden USA

Vicente G, Coteron A, Martinez M, Aracil J. 1998. Application of the factorial design of experiments and response surface methodology to optimize biodiesel production. Industrial Crops and Products 8, 29-35.

Xiao M, Mathew S, Obbard JP. 2009. Biodiesel fuel production via transesterification of oils using lipase biocatalyst. GCB Bioenergy 1, 115–125.

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