Bioefficacy of botanical extracts and bioagents against sclerotial isolates of Rhizoctonia solani

Paper Details

Research Paper 01/06/2014
Views (208) Download (7)

Bioefficacy of botanical extracts and bioagents against sclerotial isolates of Rhizoctonia solani

Azhar Hussain, Muhammad Saeed Awan, Sher Wali Khan, Muhammad Anees, Sartaj Ali, Qammar Abbas, Amjad Ali
J. Bio. Env. Sci.4( 6), 370-380, June 2014.
Certificate: JBES 2014 [Generate Certificate]


The antifungal efficacy of six botanical extracts viz., Cannabis sativa L., Peganum harmala L., Datura starmonium L., Artemisia brevifolium L., Capparis spinosa L., Mentha royleana L. and two bioagents viz., Trichoderma harizanum and Trichoderma viride were evaluated in vitro against sclerotial isolates of Rhizoctonia solani causing black scurf of potato through food poison and dual culture technique, respectively. The data revealed that increasing concentration form 5 to 15% of botanical extract suppressed the mycelial growth of all isolates. A highest antifungal property was found in C. sativa which was followed by P.harmala and D. starmonium while least in Capparis spinosa. Mycelial inhibition range in the concentrations of 5-15% were recorded in C. sativa (36.43-80.00%) P.harmala (26.71-69.20%), D. starmonium (26.44-70.28 %), A.brevifolium (24.04-66.67%) C. spinosa (21.70-50.16%) and M. royleana (24.14-61.94). Highest sensitivity against botanical extracts was observed in isolates RS10, RS4 and RS5 while least in RS12 and RS16 at concentration of 5, 10 and 15% respectively. Among the tested bioagents mycelial growth inhibition of R. solani isolates was recorded in case of T.harzianum (48.32-72.72%) and T.viride (28.75-56.80%). T. harzanium caused highest mycelial inhibition in isolate RS10 and least effective in isolate RS4 whereas T.viride was most effective in isolates RS3 and least in isolate RS15.


Adebanjo A. Bankole SA. 2004. Evaluation of some fungi and bacteria for biocontrol of anthracnose disease of cowpea. Journal of Basic Microbiology. 44(1), 3-9.

Alice D. Ramamorthy V. Muthusamy M. Seetharaman K. 1998. Biological control of Jasmine wilt incited by Sclerotium rolfsii. Indian Journal of Plant Protection, 26, 91-95.

Aqib S, Shah M. 2014. Phytochemistry, pharmacological and traditional uses of Datura stramonium L. Review. Journal of Pharmacognosy and Phytochemistry, 2(5), 123-125.

Arcury TA, Quandt SA. 2003. Pesticides at work and at home: exposure of migrant farm workers. Lancet 362 (9400), p. 2021.

Beagle-Ristaino JE. Papavizas GC. 1885. Biological controls of Rhizoctonia stem canker and black scurf of potato. Phytopathology 75, 560-564.

Baker KF. 1981. Biological control. In; Fugal wilt diseases of plants. (Eds.) Kace, M.E; Bell, A.A and Beckman, C.H. Academic Press.

Basin H. Ozturk SB. Yegen O. 1999. Efficacy of a biological fungicide (Planter Box Trichoderma harzianum Rifai T=22) against seedling root rot pathogens (Rhizoctonia solani, Fusarium sp. of cotton,” GAP-Environmental Symposium, Sanleurfa, Turkey, p. 137-144.

Bhutta AR, Khan MQ, Muhammad J, Hussain I. 2004. Pathological survey of potato crop in Northern Areas. 2003 FSC & RD/AKRSP, DOA, Islamabad, 109 p.

Chet I. 1987. Trichoderma – application, mode of action and potential as a biocontrol agent of soil borne plant pathogenic fungi In: I. Chet (Ed.), p. 137-160.

Ciampi L. Radic S. Alvarez E. 2006. Patología Vegetal Micológica. Editorial Nuova Firenze. Valdivia, Chile. p. 266.

Coskuntuna A. Ozer N. 2008. Biological control of onion basal rot disease using Trichoderma harzianum and induction of antifungal compounds in onion set following seed treatment. Crop Protection, 27, 330–336.

Cowan MM. 1999. Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12, 564-582. DOI 0893-8512/99/$04.0010.

Das K. Tiwari RKS. Shrivastava DK. 2010. Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends. Journal of Medicinal Plants Research, 4, 104-111. DOI: 10.5897/JMPR09.030

Demirci E. Eken C. 1995. The effect of tuber borne and soilborne inoculum of Rhizoctonia solani Kühn on disease severity on potato. 7th Turkish Phytopathological Congress (26-29 September 1995, Adana-Turkey): 39-43.

Deising HB.Rei mann S. Pascholati SF. 2008. Mechanisms and significance of fungicide resistance. Brazilian Journal of Microbiology, 39 (2), 286–295.

Elad Y. Chet I. Katan J. 1980. Trichoderma harzianum, a biocontrol agent effective against Sclerotium rolfsii and Rhizoctonia solani. Phytopathology 70:2 119-121. DOI 0031-979X/80/02011903/$03.00/0

ElSohly MA. 1985. Cannabis alkaloids. In: Alkaloids, chemical and biological perspectives. Pelletier S.W. Ed. John Wiley & Sons, NY 3, 169-184.

ElSohly MA. Slade D. 2005. Chemical constituents of marijuana: The complex mixture of natural cannabinoids. Life Sciences, 78, 539 – 548. doi:10.1016/j.lfs.2005.09.011

Flores-Sanchez   IJ. Verpoorte   R. 2008. Secondary metabolism in cannabis Review. Phytochemistry 2008, 7,   615. DOI:10.1007/s11101-008-9094-4.

Ganie SA. Ghani MY. Nissar Q. Rehman S. 2013. Bioefficacy of plant extracts and biocontrol agents against Alternaria solani. African Journal of Microbiology, 7(34), 4397-4402. DOI: 10.5897/AJMR2013.5901.

Grosch R. Faltin F. Lottmann J. Kofoet A. Berg G. 2005. Effectiveness of 3 antagonistic bacterial isolates to control Rhizoctonia solani Kühn on lettuce and potato. Canadian Journal of Microbiology 51, 345-353.

Han JS. Cheng JH. Yoon TM. Song J. Rajkarnikar A. Kim WG. Yoo ID. Yang YY. Suh, JW. 2005. Biological control agent of common scab disease by antagonistic strain Bacillus sp. sunhua. Journal of Applied Microbiology, 99, 213-221.

Hillig KW. 2005. Genetic evidence for separation in Cannabis (Cannabaceae). Genet Resour natural cannabinoids, Life Science, 78, 539–548. doi:10.1016/j.lfs.2005.09.011

Howell CR. 2003. Mechanisms employed by Trichoderma species in the biological control of Plant disease: the history and evolution of current concepts. Plant Disease. 87(1), 4-10.

Karunanithi K. Usman KM. 1999. Screening of Trichoderma spp. Against Fusarium oxysporium f.sp. sesame causing wilt in seasmum. Crop Research (Histar), 18, 127 – 130.

Keijer J. Korsman M. Dulleman A. Houterman P. DeBree JY. Van Silfhout C. 1997. Invitro analysis of non host plant specifity in Rhizoctonia solani. Plant Pathology 46, 659-669.

Kuc J. Shain L. 1977. Antifungal compound associated with disease resistance in plants. In: Antifungal compounds Vol. 2: Interactions in biological control and ecological systems. Siegel, M.R. and H.D. Sister (Eds.). Marcel Dekker, Inc, New York. p. 497-535.

Kumar V. Shah T. Parmar NS. Shah GB. Goyal RK. 2005: Antimicrobial activity of Peganum harmala. Indian Journal of Natural Products 21, 24-26.

Manmohan M. Govindaiah S. 2012. In vitro screening of aqueous plant extracts against Fusarium solani (Mart.) Sacc. causing root rot in mulberry. Green Farming Intternational Journal of Applied Agriculture and Horticultural Science, 3(2), 223-225.

Mathur K. Gurjar RBS. 2002. Evaluation of different fungal antagonists, plant extracts and oil cakes against Rhizoctonia solani causing stem rot of chilli. Annals of Plant Protection Sciences 10, 319-322.

Pal GK. Kumar B. 2013. Antifungal activity of some common weed extracts against wilt causing fungi, Fusarium oxysporum. International journal of current discoveries and innovations, 2(1), 62-67.

Papavizas GC. 1985. Trichoderma and Gliocladium: Biology, ecology and potential for biocontrol. Annual Review of Phytopathology, 23, 23-54. DOI:10.1146/

Samuel C. Srivastava LJ. Tripathi SC. 1995. Protection of dry fruits fungal infestation by essential oils of Coleus ambioinicus. Indian Phytopathology 3, 174-179.

Sanjay G. Tiku AK. 2009. Botanicals in Pest Management Current Status and Future Perspectives, Biomedical and life science, p.317.

Sarpeleh A. Sharifi K. Sonbolkar A. 2009. Evidence of antifungal activity of wild rue (Peganum harmala L.) on phytopathogenic fungi, Journal of Plant Diseases and Protection, 116(5), 208–213.

Shahnaz D. Sadia K. Marium T. 2010. Comparative effect of plant extracts of Datura alba Ness and Cynodon dactylon (L.) Pers., alone or in combination with microbial antagonists for the control of root disease of cowpea and okra. Pakistan Journal of Botany 42(2), 1273-1279.

Shalini S. Kotasthane AS. 2007. Parasitism of Rhizoctonia solani by strains of Trichoderma spp. Electronic Journal of Agriculture and Food chemistry, 6(8), 2272-2281.

Shivpuri A. Gupta RBL. 2001. Evaluation of different fungicides and plant extracts against Sclerotinia sclerotiorum causing stem rot of mustard. Phytopathology 52, 272-274

Vincent JM. 1947. Distribution of fungal hyphae in the presence of certain inhibitors. Nature 15, 850.

Weller DM. Raaijmakers JM. Gardener BB. Thomashow LS. 2002. Microbial populations responsible for specific soil suppressiveness to plant pathogens. Annual Review Phytopathology, 40, 309-348.

Zaidi MI. Alvina G. Shaukat A. 2004: Antifungal activity of harmine, HgCl2 and their complex. Sarhad Journal of agriculture, 20, 623-626.