Biofloc-based nursery production system: heeding the call towards a sustainable shrimp culture industry in the Philippines

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Biofloc-based nursery production system: heeding the call towards a sustainable shrimp culture industry in the Philippines

Christopher Marlowe A. Caipang, Kathleen Mae P. Trebol, Fernand F. Fagutao, Rolando V. Pakingking, Jr., Joel E. Deocampo, Jr
Int. J. Biosci.20( 3), 250-259, March 2022.
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The increasing global population resulted in intense pressure on the food production sectors to meet the rise in food demand. The aquaculture industry, which is one of the major food production sectors, provides opportunities in addressing issues on malnutrition and poverty alleviation. Shrimp farming is an important sub-sector in aquaculture because shrimp are not only good sources of food, but they contribute to the national economy through export revenues. This resulted in the rapid intensification of shrimp aquaculture, which created negative issues on sustainability and environmental impacts. Hence, this necessitates an urgent need to develop aquaculture production systems that yield high productivity and profitability yet possess a low carbon footprint. Biofloc technology (BFT) fit into these criteria as this technology permits intensive culture of aquatic species, less use of resources, and improved water quality as a consequence of the production and activity of beneficial microbial biomass, which, at the same time, can be utilized as a source of feed for the growing shrimp. BFT has been shown to be successful on a commercial scale during shrimp grow-out, and recent studies have shown that this technology can be further refined and optimized for the production of shrimp during the nursery phase. This review, therefore, highlights the basics of BFT and how this technology is being optimized in the production of shrimp during the nursery phase. More specifically, this discusses the benefits of this approach in ensuring a productive yet sustainable way of producing shrimp in the context of Philippine aquaculture.


Apostol-Albaladejo MAG. 2016. Status of acute hepatopancreatic necrosis disease (AHPND) of cultured shrimps in the Philippines. In Addressing Acute Hepatopancreatic Necrosis Disease (AHPND) and Other Transboundary Diseases for Improved Aquatic Animal Health in Southeast Asia: Proceedings of the ASEAN Regional Technical Consultation on EMS/AHPND and Other Transboundary Diseases for Improved Aquatic Animal Health in Southeast Asia, 22-24 February 2016, Makati City, Philippines (pp. 65-72). Aquaculture Department, Southeast Asian Fisheries Development Center.

Arnold SJ, Coman FE, Jackson CJ, Groves SA. 2009. High-intensity, zero water-exchange production of juvenile tiger shrimp, Penaeus monodon: an evaluation of artificial substrates and stocking density. Aquaculture 293, 42–48.

Asaduzzaman M, Wahab MA, Verdegem MCJ, Adhikary RK, Rahman SMS, Azim ME. 2010. Effects of carbohydrate source for maintaining a high C:N ratio and fish driven re-suspension on pond ecology and production in periphyton-based freshwater prawn culture systems. Aquaculture 301, 37–46.

Avnimelech Y. 1999. Carbon/nitrogen ratio as a control element in aquaculture systems. Aquaculture 176, 227–235.

Avnimelech Y. 2007. Feeding with microbial flocs by tilapia in minimal discharge bio-flocs technology ponds. Aquaculture 264 (1-4), 140-147.

Avnimelech Y. 2015. Biofloc Technology. A Practical Guidebook, 3rd edn. The World Aquaculture Society, Baton Rouge, LA.

Avnimelech Y, Mokady S, Schroeder GL. 1989. Circulated ponds as efficient bioreactors for single-cell protein production. Israel Journal of Aquaculture, Bamidgeh 41, 58-66.

Azim ME, Little DC. 2008. The biofloc technology (BFT) in indoor tanks: water quality, biofloc composition, and growth and welfare of Nile tilapia (Oreochromis niloticus). Aquaculture 283, 29–35.

Barcenal ARB, Traifalgar RFM, Corre Jr VL. 2015. Anti-Vibrio harveyi property of Micrococcus luteus isolated from rearing water under biofloc technology culture system. Current Research in Bacteriology 8(2), 26-33.

Browdy CL, Bratvold D, Stokesland AD, McIntosh P. 2001. Perspectives on the application of closed shrimp culture systems. In: Browdy CL, Jory DE (eds) New Wave, Proceedings of the Special Session on Sustainable Shrimp Farming, p 20–34. World aqua. Soc., Baton Rough, LA.

Burford MA, Thompson PJ, McIntosh RP, Bauman RH, Pearson DC. 2004. The contribution of flocculated material to shrimp (Litopenaeus vannamei) nutrition in a high-intensity, zero-exchange system. Aquaculture 232, 525–537.

Cadiz RE, Traifalgar RFM, Sanares RC, Andrino-Felarca KGS, Corre Jr VL. 2016. Comparative efficacies of tilapia green water and biofloc technology (BFT) in suppressing population growth of green Vibrios and Vibrio parahaemolyticus in the intensive tank culture of Penaeus vannamei. Aquaculture, Aquarium, Conservation & Legislation 9(2), 195-203.

Caipang CMA, Choo HX, Bai Z, Huang H and Lay-yag CM. 2015. Viability of sweet potato flour as carbon source for the production of biofloc in freshwater culture of tilapia, Oreochromis sp. International Aquatic Research 7, 329-336.

Chen QL, Zhang RJ, Wang YH. 2015. The Effect of biofloc technology on nursery system of Litopenaeus vannamei. Applied Mechanics and Materials 737, 358–361.

Choo HX, Caipang CMA. 2015. Biofloc technology (BFT) and its application towards improved production in freshwater tilapia culture. Aquaculture, Aquarium, Conservation & Legislation 8, 362-366.

Crab R, Chielens B, Wille M, Bossier P, Verstraete W. 2010. The effect of different carbon sources on the nutritional value of bioflocs, a feed for Macrobrachium rosenbergii postlarvae. Aquaculture Research 41, 559–567.

Crab R, Defoirdt T, Bossier P, Verstraete W. 2012. Biofloc technology in aquaculture: beneficial effects and future challenges. Aquaculture 356, 351-356.

Cuzon G, Lawrence A, Gaxiola G, Rosas C, Guillaume J. 2004. Nutrition of Litopenaeus vannamei reared in tanks or in ponds. Aquaculture 235, 513–551.

Ekasari J, Angela D, Waluyo SH, Bachtiar T, Surawidjaja EH, Bossier P. 2014. The size of biofloc determines the nutritional composition and the nitrogen recovery by aquaculture animals. Aquaculture 426–427, 105–111.

ElSayed AFM. 2021. Use of biofloc technology in shrimp aquaculture: a comprehensive review, with emphasis on the last decade. Reviews in Aquaculture 13(1), 676-705.

Emerenciano M, Ballester ELC, Cavalli RO, Wasielesky W. 2011. Effect of biofloc technology (BFT) on the early postlarval stage of pink shrimp Farfantepenaeus paulensis: growth performance, floc composition and salinity stress tolerance. Aquaculture International 19, 891–901.

Emerenciano M, Ballester ELC, Cavalli RO, Wasielesky W. 2012. Biofloc technology application as a food source in a limited water exchange nursery system for pink shrimp Farfantepenaeus brasiliensis (Latreille, 1817). Aquaculture Research 43, 447–457.

Emerenciano M, Gaxiola G, Cuzon G. 2013. Biofloc technology (BFT): a review for aquaculture application and animal food industry. Biomass now-cultivation and utilization, pp.301-328.

FAO. 2016. The state of world fisheries and aquaculture 2016. Contributing to food security and nutrition for all. FAO, Rome. p 200.

FAO. 2020. Global Aquaculture Production 1950–2018.

Hargreaves JA. 2006. Photosynthetic suspended-growth systems in aquaculture. Aquacultural Engineering 34, 344–363.

Hargreaves JA. 2013. Biofloc production systems for aquaculture. Stoneville, MS: Southern Regional Aquaculture Center.

Jang IK, Pang Z, Yu J, Kim SK, Seo HC, Cho YR. 2011. Selectively enhanced expression of prophenoloxidase activating enzyme 1 (PPAE1) at a bacteria clearance site in the white shrimp, Litopenaeus vannamei. BMC immunology 12(1), 1-11.

Ju ZY, Forster I, Conquest L, Dominy W, Kuo WC, Horgen FD. 2008. Determination of microbial community structures of shrimp floc cultures by biomarkers and analysis of floc amino acid profiles. Aquaculture Research 39, 118–133.

López-Elías JA, Moreno-Arias A, Miranda-Baeza A. 2015. Proximate composition of bioflocs in culture systems containing hybrid Red tilapia fed diets with varying levels of vegetable meal inclusion. North American Journal of Aquaculture 77, 102–109.

Luo GZ, Gao Q, Wang CH, Liu WC, Sun DC, Li L, Tan HX. 2014.  Growth, digestive activity, welfare, and partial cost-effectiveness of genetically improved farmed tilapia (Oreochromis niloticus) cultured in a recirculating aquaculture system and an indoor biofloc system. Aquaculture 422-423, 1-7.

Michaud L, Blancheton JP, Bruni V, Piedrahita R. 2006. Effect of particulate organic carbon on heterotrophic bacterial populations and nitrification efficiency in biological filters. Aquaculture Engineering 34, 224–233.

Mishra JK, Samocha TM, Patnaik S, Speed M, Gandy RL, Ali A. 2008. Performance of an intensive nursery system for the Pacific white shrimp, Litopenaeus vannamei, under limited discharge condition. Aquacultural Engineering 38, 2-15.

Muegue MSF, Caipang CMA, Geduspan JS. 2015. Current status of shrimp aquaculture in the Philippines. In: Caipang CMA, Bacano-Maningas MBI and Fagutao FF (eds) Biotechnological Advances in Shrimp Health Management in the Philippines, Research Signpost, Kerala, India, p 1-17.

Palanca-Tan R. 2018. Aquaculture, poverty and environment in the philippines. The Journal of Social, Political, and Economic Studies 43, 294-315.

Panigrahi A, Saranya C, Sundaram M, Kannan SV, Das RR, Kumar RS. 2018.  Carbon: nitrogen (C: N) ratio level variation influences microbial community of the system and growth as well as immunity of shrimp (Litopenaeus vannamei) in biofloc based culture system. Fish & Shellfish Immunology 81, 329–337.

Rathore SS, Yusufzai SI and Katira NN. 2016.  Biofloc technology – the futuristic technology for improving the ecological and economic sustainability of aquaculture. Life Sciences International Research Journal 3, 29-32.

Samocha TM, Patnaik S, Speed M, Ali AM, Burger JM, Almeida RV. 2007. Use of molasses as carbon source in limited discharge nursery and grow-out systems for Litopenaeus vannamei. Aquacultural Engineering 36, 184–191.

Serra FP, Gaona CA, Furtado PS, Poersch LH, Wasielesky W. 2015. Use of different carbon sources for the biofloc system adopted during the nursery and grow-out culture of Litopenaeus vannamei. Aquaculture International 23, 1325–1339.

Wasielesky W, Atwood H, Stokes A, Browdy CL. 2006. Effect of natural production in a zero exchange suspended microbial floc based super-intensive culture system for white shrimp Litopenaeus vannamei. Aquaculture 258, 396–403.

Wasielesky W, Froes C, Foes G, Krummenauer D, Lara G, Poersch L. 2013. Nursery of Litopenaeus vannamei reared in a biofloc system: the effect of stocking densities and compensatory growth. Journal of Shellfish Research 32, 799–806.

Xu WJ, Pan LQ. 2013. Enhancement of immune response and antioxidant status of Litopenaeus vannamei juvenile in biofloc-based culture tanks manipulating high C/N ratio of feed input. Aquaculture 412, 117-124.

Yun H, Shahkar E, Hamidoghli A, Lee S, Won S, Bai SC. 2017. Evaluation of dietary soybean meal as fish meal replacer for juvenile whiteleg shrimp, Litopenaeus vannamei reared in biofloc system. International Aquatic Research 9(1), 11-24.