Paper Details

Biological activities of a new antimicrobial peptide from Coriandrum sativum

Masoud Zare-Shehneh, Mohammad Askarfarashah, Leila Ebrahimi, Nasroallah Moradi Kor, Hadi Zare-Zardini, Hossein Soltaninejad, Zahra Hashemian, Fatemeh Jabinian

DOI: https://dx.doi.org/10.12692/ijb/4.6.89-99

Int. J. Biosci. 4(6), 89-99. March, 2014. (PDF)

Abstract:

Antimicrobial compounds from plant sources have varied therapeutic potential, as investigation on which has today been becoming an active field. Purification of peptides was done with gel filtration and reverse-phase high-performance liquid chromatography (RP-HPLC). Antimicrobial activity of new antimicrobial peptide derived from coriander leaf extract has been investigated against different organisms by Radial Diffusion Assay (RDA) and Minimum Inhibitory Concentration (MIC). In the present study, a novel antimicrobial peptide with a wide antimicrobial activity was isolated from coriander leaf extract and the greatest antimicrobial effect of it was shown on Staphylococcus aureus strain with MIC 1.3 mg/ml which is an antibiotic resistant prevalent in hospitals. The new peptide showed effective germicidal effects on Klebsiella pneumonia and halted bacterial growth in concentration 2.65 mg/ml. Among the strains investigated, Pseudomonas aeruginosa was the most resistant strain with MIC of 3.2 mg/ml. This peptide was also influential on the fungi. There was no growth in concentration of 2.5 and 2.3 mg/ml for Penicillium lilacinum and Asperjilus niger, respectively. This peptide showed no hemolytic activity against human red blood cells. By multiple sequence alignment, this new peptide was named Plantaricin CS. Different agents have known in the extract of Coriandrum sativum that have potent biological activity such as antimicrobial activity. The new peptide was acquired in this work, has wide antimicrobial and low hemolytic activity. Thus, this peptide may be a useful drug in treatment infection diseases.