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Biological evaluation and preliminary screening of endophytes of Taxus fauna for taxol production

Muniba Jadoon, Nighat Fatima, Ibrar Khan, Bushra Mirza, Ihsan-Ul-Haq, Muneer Ahmed Qazi, Safia Ahmed

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Int. J. Biosci.7(5), 38-46, November 2015

DOI: http://dx.doi.org/10.12692/ijb/7.5.38-46


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Endophytic fungi of Taxus species, being considered as economically most promising source for taxol production, prompted the current study for screening of endophytic fungi associated with Taxus fuana of Pakistan. Two endophytic fungi Epicoccum sp.NFW1 and MucorhemalisNFW6 were explored for their biological activities and taxol production by utilizing bioassays, TLC, RP-HPLC and LC-ESI-MS analyses. Crude extract of NFW1 showed significant antimicrobial activity with zone of inhibition ranging from 11.8 – 23.1 mm against different test strains. It also expressed cytotoxicity in brine shrimp lethality assay with an IC50 value 10µg/mL. NFW6 expressed moderate antimicrobial activity and high antioxidant potential (89.2%) with IC50 51.2 µg/mL in DPPH assay. RP-HPLC confirmed the presence of taxol in NFW1 at a concentration 280.7 µg/g of dry mycelial mass. It was further validated by LC-ESI-MS analysis. These findings reveal that Epicoccum sp.NFW1 should be studied for the isolation and characterization of taxol and related metabolites.


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Biological evaluation and preliminary screening of endophytes of Taxus fauna for taxol production

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