Biological evaluation and preliminary screening of endophytes of Taxus fauna for taxol production

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Research Paper 01/11/2015
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Biological evaluation and preliminary screening of endophytes of Taxus fauna for taxol production

Muniba Jadoon, Nighat Fatima, Ibrar Khan, Bushra Mirza, Ihsan-Ul-Haq, Muneer Ahmed Qazi, Safia Ahmed
Int. J. Biosci.7( 5), 38-46, November 2015.
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Endophytic fungi of Taxus species, being considered as economically most promising source for taxol production, prompted the current study for screening of endophytic fungi associated with Taxus fuana of Pakistan. Two endophytic fungi Epicoccum sp.NFW1 and MucorhemalisNFW6 were explored for their biological activities and taxol production by utilizing bioassays, TLC, RP-HPLC and LC-ESI-MS analyses. Crude extract of NFW1 showed significant antimicrobial activity with zone of inhibition ranging from 11.8 – 23.1 mm against different test strains. It also expressed cytotoxicity in brine shrimp lethality assay with an IC50 value 10µg/mL. NFW6 expressed moderate antimicrobial activity and high antioxidant potential (89.2%) with IC50 51.2 µg/mL in DPPH assay. RP-HPLC confirmed the presence of taxol in NFW1 at a concentration 280.7 µg/g of dry mycelial mass. It was further validated by LC-ESI-MS analysis. These findings reveal that Epicoccum sp.NFW1 should be studied for the isolation and characterization of taxol and related metabolites.


Backman PA, Sikora RA. 2008. Endophytes: An emerging tool for biological control. Biological Control 46, 1–3.

Bhimba VB, Franco DA, Mathew JM, Jose GM, Joel EL. 2010. Anticancer and antimicrobial activity of mangrove derived fungi Hypocrealixii VB1. ChineseJournal ofNaturalMedicines 10, 77-80.

Cardellina JH. 1991. HPLC separation of taxol and cephalomannine. Journal of Chromatography 14, 659−665.

Fávaro LC, De Souza Sebastianes FL, Araújo WZ. 2012. Epicoccumnigrum P16,a sugarcane endophyte, produces antifungal compounds and induces root growth. PLoS One 7(6), e36826.

Gangadevi V, Muthumary J. 2008. Isolation of Colletotrichumgloeosporioides, a novel endophytic Taxol-producing fungus from Justiciagendarussa. MycologiaBalcanica 5, 1-4.

Gangadevi V, Murguan M, Muthumary J. 2008. Taxol determination from Pestalotiopsispauciseta, a fungal endophyte of a medicinal plant. Chinese Journal of Biotechnology 24(8), 1433-1438.

Gunatilaka  AAL.  2006.  Natural  products  from plant-associated microorganisms: Distribution, structural diversity, bioactivity and important implication of their occurrence. Journal of Natural Products 69, 509-526.

Haq IU, Ullah N, Bibi G, Kanwal S, Ahmad M S, Mirza B. 2012. Antioxidant and cytotoxic activities and phytochemical analysis of Euphorbiawallichii root extract and its fractions. Iranian Journal of Pharmaceutical Research  11(1),241.

Hazalin NA, Ramasamy K, Lim SM, Wahab IA, Cole ALJ, Majeed ABA. 2009. Cytotoxic and antibacterial activities of endophytic fungi isolated from plants at the National Park, Pahang, Malaysia. BMC Complementary and Alternative Medicine 9, 46.

Hoffman AM, Mayer SG, Strobel GA, Hess WM, Sovocool GW, Grange AH, Kelley Swift EG. 2008. Purification, identification and activity of phomodione, a furandione from an endophyticPhoma species. Phytochemistry 69(4), 1049–56.

Joseph B, Priya RM. 2011. Bioactive compounds from endophytes and their potential in pharmaceutical effects: a review. American Journal of Biochemistry and Molecular Biology 1(3), 291-309.

Kanan GJ, Al-Najar RA. 2008. In vitro antifungal activities of various plant crude extracts and fractions against citrus post-harvest disease agent Penicilliumdigitatum. Jordan  Jorunal  of  Biological Sciences 1(3), 89-99.

Khan R, Saleem S, Muhammad IC, Shakeel K, Ahmed A. Communities of endophytic fungi in medicinal plant Withaniasomnifera. Pakistan Journal of Botany 42(2), 1281-1287.

Kumar S, Aharwah RP, Shuklai H, Rajak RC, Sandhu SS. 2013. Endophytic fungi: as source of antimicrobials bioactive compounds. World Journal of Pharmacy and Pharmaceutical Sciences 3(2), 1179-1197.

Kumaran  RS,  Kim  HJ,  Hur  BK.  2010.Taxol producing (corrected) fungal endophyte, Pestalotiopsis species isolated from Taxus cuspidata. Journal of Bioscience andBioengineering 110, 541–546.

Liu X, Dong M, Chen X, Jiang M, Lv X, Yan G. 2007. Antioxidant activity and phenolics of an endophyticXylaria sp. from Ginkgo biloba. FoodChemistry 105(5), 548-554.

Pandi M, Kumaran RS, Choi YK, Kim HJ, Muthumary J. 2011. Isolation and detection of taxol, an anticancer drug produced from Lasiodiplodiatheobromae, an endophytic fungus of the medicinal plant Morindacitrifolia. African Journal of Biotechnology 10(8), 1428-1435.

Mukherjee S, Ghosh B, Jha TB, Jha S. 2002. Variation in content of taxol and related taxanes in Eastern Himalayan populations of Taxus wallichiana. Planta Medica 68(8), 757-759.

Musetti R, Polizzotto R, Grisan S, Martini M, Borselli S, Carraro L, Osler R. 2007. Effects induced by fungal endophytes in Catharanthusroseus tissues infected by phytoplasmas. Bulletin of Insectology 60(2), 293-294.…/vol60-2007-293-294musetti.pdf

Pimentel MR, Gustavo M, Ana PD, Mário RMJ, Gláucia MP. 2011. The use of endophytes to obtain bioactive compounds and their application in biotransformation process. Biotechnology Research International 2011, 1-11. 0.4061/2011/576286

Pimentel MR, Molina G, Dionisio PA, Marostica Junior MR, Pastore GM. 20 1 0 . Theuseof endophytestoobtainbioactive compoundsandtheir applicationin biotransformation process: review article. Biotechnology Research International 2011, 1–11.

Poncharoen V, Rukachaisirikul S, Phongpaichit T, Kuhn M , Matthias P, Jariya S, Walter T. 2008. Metabolites from the endophytic fungus Xylaria sp. PSU-D14.Phytochemistry 69(9), 1900–1902.

Qadri M, Roopli R, Malik ZA, Ram AV, Syed RH. 2014. Diversity, molecular phylogeny and bioactive potential of fungal endophytes associated with the Himalayan Blue Pine (Pinuswallichiana). Microbial Ecology 67, 877-887.

Roberts SC. 2007. Production and engineering of terpenoids in plant cell culture. Nature Chemical Biology 3, 387 – 395.

Shah A, Li DZ, Moller M, Gao LM, Hollingsworth ML. 2008. Delimitation of Taxusfuana Nan Li RR Mill (Taxaceae) based on morphological and molecular data. Taxon 57, 211-222.

Soliman SS, Raizada MN. 2013. Interactions between co-habitating fungi elicit synthesis of Taxol from an endophytic fungus in host Taxus plants. Frontiers in Microbiology 4(3), 1-14.

Stierle A, Strobel G, Stierle D. 1993. Taxolandtaxaneproductionby Taxomycesandreanae, anendophytic fungus of Pacificyew. Science 260(5105), 214-216.

Strobel  G,  Daisy  B.  2003.  Bioprospecting  for microbial endophytes and their natural products. Microbiology and Molecular Biology Reviews 67(4), 491-502.

Strobel G, Yang X, Sears J, Kramer R, Sidhu RS, Hess WM. 1996. Taxol from Pestalotiopsismicrospore, an endophytic fungus of Taxuswallachiana. Microbiology 142, 435-440.

Strobel  GA,  Daisy B,  Castillo  U,  Harper J. 2004. Natural products from endophytic microorganisms. Journal of Natural Products 67(2), 257-268.

Tan RX, Zou WX. 2001. Endophytes: a rich source of functional metabolites. Natural Product Reports 18(4), 448-459.

Tayung K, Jha DK. 2010. Antimicrobial endophytic  fungal  assemblages  inhabiting  bark  of Taxus  baccataL  of  Indo-Burma  mega  biodiversity hotspot. Indian Journal of Microbiology 50(1), 74-81.

Wang J, Guling J, Huaying L, Zhonghui Z, Yaojian H, Wenjin S. 2000. Taxol from Tubercularia sp. strain TF5, an endophytic fungus of Taxusmairei. FEMS Microbiology Letters 193, 249-253. x

Wani MC, Taylor HL, Wall ME, Coggen P, McPhail AT. 1971. Plant antitumor agents VI. The isolation and structure of taxol, a novel antileukemic and anticancer agent from Taxus brevifolia.Journal of American Chemical Society 93, 2325-2327.

Wright AD, Osterhage C, König GM. 2003. Epicoccamide, a novel secondary metabolite from a jelly fish derived culture of Epicoccumpurpurascens. Organic and Biomolecular Chemistry 1(3), 507-510.

Xiong ZQ, Yang YY, Zhao N, Wang Y. 2013. Diversity of endophytic fungi and screening of fungal paclitaxel producer from Anglojap yew, Taxus media.BMC Microbiology 13, 71.

Xu F, Wenyi T, Long C, Lijia G. 2006. Strain improvement and optimization of the media of taxol-producing fungus Fusariummarie. Biochemical Engineering Journal 31, 67-73.

Yu HS, Zhang L, Li L, Zheng CJ, Guo L, Li WC, Sun PX, Qin LP. 2010. Recent developments and future prospects of antimicrobialmetabolites produced by endophytes. Microbiological Research 165(6), 437-449.

Zhang H, Bai X, Wu B. 2012. Evaluation of antimicrobialactivitiesofextracts of endophyticfungi fromArtemisia annua.Bangladesh Journal of Pharmacology 7(2), 120-123.