Biopesticides potential to protect tomato (Solanum lycopersicum L.) production from early blight disease (Alternaria solani) and leaf miners (Tuta absoluta)
Paper Details
Biopesticides potential to protect tomato (Solanum lycopersicum L.) production from early blight disease (Alternaria solani) and leaf miners (Tuta absoluta)
Abstract
Early blight disease (Alternaria solani) and leaf miners (Tuta absoluta) pose significant biotic constraints to tomato production, causing 80 -100% yield loss. A study involving laboratory, screen house and field experiments evaluated the efficacy of plant and bio-product pesticides including extracts of Azadirachta indica, Lantana camara, Cymbopogon citratus, Capsicum frutescens, Zingiber officinale and rabbit urine. We report significant inhibition of fungal mycelial growth ranging from 20% with 5% rabbit urine to 98.25% by 10% hot pepper in the laboratory. Screen-house trials showed a positive inhibitory effect of plant extracts on controlling leaf miners. The most effective extracts were 10% lantana (80%), 10% hot pepper (76.6%) and 10% ginger (72.6%). The most successful four extracts were used in field trials and resulted in an average 50% reduction in disease severity compared to the control. Also, 10% ginger, hot pepper (both 5% and 10%) and 10% lantana were effective in controlling Tuta absoluta and significantly reduced leaf damage. A cost benefit analysis showed that the 5% hot pepper treatment had the highest revenue benefit compared to the negative control and conventional tomato production methods. Further research is needed to integrate these biopesticides into crop management practices.
Abada KA, Mostafa SH, Mervat R. 2008. Effect of some chemical salts on suppressing the infection by early blight disease of tomato. Egyptian Journal of Applied Science 23(20), 47–58.
Abdel-Kader MM, El-Mougy NS. 2016. Potential control of beans (Phaseolus vulgaris L.) wilt disease using growth regulators, bioagent, antioxidants and essential oils as foliar application under field conditions. African Journal of Microbiology Research 10(46), 1952–1960.
Abd-El-Khair H, Haggag M. 2007. Application of some Egyptian medicinal plant extracts against potato late and early blights. Research Journal of Agriculture and Biological Sciences 3, 166–175.
Abhilash PC, Singh N. 2009. Pesticide use and application: An Indian scenario. Journal of Hazardous Materials 165(1–3), 1–12.
Abo-Elyousr KA, Ali EF, Sallam NM. 2022. Alternative control of tomato wilt using the aqueous extract of Calotropis procera. Horticulturae 8(3), 197.
Abubakar AR, Haque M. 2020. Preparation of medicinal plants: Basic extraction and fractionation procedures for experimental purposes. Journal of Pharmacy and Bioallied Sciences 12(1), 1–10.
Agboola AR, Okonkwo CO, Agwupuye EI, Mbeh G. 2022. Biopesticides and conventional pesticides: Comparative review of mechanism of action and future perspectives. AROC Agric 1, 14–32.
Ahmad F, Raziq F, Ullah N, Khan H, Din N. 2017. In vitro and in vivo bio-assay of phytobiocidal effect of plant extracts on Alternaria solani causing agent of early blight disease in tomato. Archives of Phytopathology and Plant Protection 50(11–12), 568–583.
Alam MJ, Ahmed KS, Rony MNH, Islam NET, Bilkis SE. 2019. Bio-efficacy of bio-pesticides against tomato leaf miner, Tuta absoluta, a threatening pest of tomato. Journal of Bioscience and Agriculture Research 22(2), 1852–1862.
Alam MS, Islam MT, Khatun MA, Alam SS. 2016. In vitro antifungal activity of ginger extract and gingerol against Alternaria solani. Journal of Applied Microbiology 120(2), 528–536.
Ashraf R, Sultana B, Riaz S, Mushtaq M, Iqbal M, Nazir A, Zafar Z. 2018. Fortification of phenolics, antioxidant activities and biochemical attributes of radish root by plant leaf extract seed priming. Biocatalysis and Agricultural Biotechnology 16, 115–120.
Assinapol N. 2020. Evaluation of entomopathogens and plant extracts as options for integrated pest management of Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) for enhanced tomato productivity in Rwanda. Doctoral dissertation, Egerton University.
Ayilara MS, Adeleke BS, Akinola SA, Fayose CA, Adeyemi UT, Gbadegesin LA, Babalola OO. 2023. Biopesticides as a promising alternative to synthetic pesticides: A case for microbial pesticides, phytopesticides, and nanobiopesticides. Frontiers in Microbiology 14, 1040901.
Badr AN, Stepien L, Drzewiecka K, Alharthi SS, Selim K, Abdel-Razek AG. 2021. Synergistic impact of bioactive byproduct extract leads to anti-Fusarium and anti-mycotoxin secretion. Journal of Fungi 8(1), 30.
Balemi T. 2008. Response of tomato cultivars differing in growth habit to nitrogen and phosphorus fertilizers and spacing on vertisol in Ethiopia. Acta Agriculturae Slovenica 91(1), 103–119.
Bilalis D, Krokida M, Roussis I, Papastylianou P, Travlos I, Cheimona N, Dede A. 2018. Effects of organic and inorganic fertilization on yield and quality of processing tomato (Mill.). Folia Horticulturae 30(2), 321–332.
Brévault T, Sylla S, Diatte M, Bernadas G, Diarra K. 2014. Leaf miner (Tuta absoluta Meyrick) (Lepidoptera: Gelechiidae): A new threat to tomato production in Sub-Saharan Africa. African Entomology 22, 441–444.
Brul S, Klis FM. 1999. Mechanistic and mathematical inactivation studies of food spoilage fungi. Fungal Genetics and Biology 27(2–3), 199–208.
Chtioui W, Heleno S, Migheli Q, Rodrigues P. 2023. Plant extracts as biocontrol agents against Aspergillus carbonarius growth and ochratoxin A production in grapes. International Journal of Food Microbiology 407, 110425.
Coronel OADÁ, León‐García E, Vela‐Gutiérrez G, Medina JDLC, García-Varela R, García HS. 2017. Chayote (Sechium edule (Jacq.) Swartz). In: Fruit and Vegetable Phytochemicals: Chemistry and Human Health, 2nd ed., 979–992.
Da Cruz Cabral L, Pinto VF, Patriarca A. 2013. Application of plant-derived compounds to control fungal spoilage and mycotoxin production in foods. Int J Food Microbiol 166(1), 1–14.
Del Valle M, Cámara M, Torija ME. 2006. The nutritional and functional potential of tomato by-products. In: X International Symposium on the Processing Tomato 758, 165–172.
FAO. 2009. FAOSTAT. Food and Agriculture Organization.
Fuentefria AM, Pippi B, Dalla Lana DF, Donato KK, de Andrade SF. 2018. Antifungals discovery: An insight into new strategies to combat antifungal resistance. Lett Appl Microbiol 66(1), 2–13.
Fufa F, Hanson P, Dagnoko S, Dhaliwal M. 2009. AVRDC-The World Vegetable Center tomato breeding in sub-Saharan Africa: Lessons from the past, present work, and future prospects. In: I All Africa Horticultural Congress 911, 87–98.
Gautam S, Schreinemachers P, Uddin MN, Srinivasan R. 2017. Impact of training vegetable farmers in Bangladesh in integrated pest management (IPM). Crop Prot 102, 161–169.
Gharib A, Davies EG, Goss GG, Faramarzi M. 2017. Assessment of the combined effects of threshold selection and parameter estimation of Generalized Pareto Distribution with applications to flood frequency analysis. Water 9(9), 692.
Giriraju A, Yunus GY. 2013. Assessment of antimicrobial potential of 10% ginger extract against Streptococcus mutans, Candida albicans, and Enterococcus faecalis: An in vitro study. Indian J Dent Res 24, 397–400.
Gopieshkhanna V, Kannabiran K. 2007. Larvicidal effect of Hemidesmus indicus, Gymnema sylvestre, and Eclipta prostrata against Culex quinquefasciatus mosquito larva. Afr J Biotechnol 6, 307–311.
Gotora T, Masaka L, Sungirai M. 2014. Effect of cow urine on the growth characteristics of Fusarium lateritium, an important coffee fungus in Zimbabwe. Int J Agron 2014, 1–6.
Haggag WM, El-Khair HA. 2007. Application of some natural compounds for management of potato late and early blights.
Hernández S, López JL. 2020. Uncertainty quantification for plant disease detection using Bayesian deep learning. Appl Soft Comput 96, 106597.
Heuvelink E, Dorais M. 2005. Crop growth and yield. In: Tomatoes. CABI Publishing, Wallingford, UK, 85–144.
Ibrahim MA, Metry EA, Moustafa SA, Tawfik AE, Nasr El-Din TM. 2004. Morphological and molecular diagnosis of commercial potato cultivar infected with Ralstonia solanacearum. Arab J Biotechnol 8, 211–222.
Ibrahim MIJ, Sapuan SM, Zainudin ES, Zuhri MYM. 2019. Extraction, chemical composition, and characterization of potential lignocellulosic biomasses and polymers from corn plant parts. BioResources 14(3), 6485–6500.
Iqbal Z, Muhammad F, Bhatti MA. 2003. The antifungal activity of neem (Azadirachta indica) oil against plant pathogenic fungi. Pestic Sci 53(8), 787–793.
Kalidindi N, Thimmaiah NV, Jagadeesh NV, Nandeep R, Swetha S, Kalidindi B. 2015. Antifungal and antioxidant activities of organic and aqueous extracts of Annona squamosa Linn. leaves. J Food Drug Anal 23(4), 795–802.
Kashiwagi T, Mikagi E, Mekuria DB, Boru AD, Tebayashi SI, Kim CS. 2005. Ovipositional deterrent on mature stage of sweet pepper, Capsicum annuum, against Liriomyza trifolii (Burgess). Z Naturforsch C 60(9–10), 739–742.
Katiyar D, Manish, Saxena Pal R, Bansal P, Kumar A, Prakash S. 2024. Electrochemical sensors for detection of phytomolecules: A mechanistic approach. Comb Chem High Throughput Screen 27(13), 1887–1899.
Kemunto D, Omuse ER, Mfuti DK, Tamiru A, Hailu G, Rwiza I, Niassy S. 2022. Effect of rabbit urine on the larval behaviour, larval mortality, egg hatchability, adult emergence, and oviposition preference of the fall armyworm (Spodoptera frugiperda JE Smith). Agriculture 12(8), 1282.
Khatun S, Islam MT, Hossain MA. 2015. Antifungal activity of gingerol against plant pathogenic fungi. Int J Chem Stud 3(5), 140–144.
Koleva-Gudeva L, Mitrev S, Maksimova V, Spasov D. 2013. Content of capsaicin extracted from hot pepper (Capsicum annuum ssp. microcarpum L.) and its use as an ecopesticide. Hem Ind 67(4), 671–675.
Koza N, Adedayo A, Babalola O, Kappo A. 2022. Microorganisms in plant growth and development: Roles in abiotic stress tolerance and secondary metabolites secretion. Microorganisms 10, 1528.
Krakowska-Sieprawska A, Kiełbasa A, Rafińska K, Ligor M, Buszewski B. 2022. Modern methods of pre-treatment of plant material for the extraction of bioactive compounds. Molecules 27(3), 730.
Liambila RN, Wesonga JM, Ngamau CN, Waudo W. 2021. Chemical composition and bioactivity of Lantana camara L. essential oils from diverse climatic zones of Kenya against leaf miner (Tuta absoluta Meyrick). Afr J Agric Res 17(9), 1198–1208.
Liambila RN. 2023. Characterisation of essential oil compounds and optimisation of water and potassium for production of Lantana camara (L.) for Tuta absoluta management. Doctoral dissertation, JKUAT-CoANRE.
Lynch K. 1999. Commercial horticulture in rural Tanzania–An analysis of key influences. Geoforum 30(2), 171–183.
Malinga LN, Laing MD. 2023. Farmers’ production practices, incidence and management of pests and diseases, extension services, and factors limiting cotton production and quality in South Africa. S Afr J Agric Ext 51(3), 79–99.
Martin AK. 1982. The origin of urinary aromatic compounds excreted by ruminants: 3. The metabolism of phenolic compounds to simple phenols. Br J Nutr 48(3), 497–507.
Mkindi AG, Coe R, Stevenson PC, Ndakidemi PA, Belmain SR. 2021. Qualitative cost-benefit analysis of using pesticidal plants in smallholder crop protection. Agriculture 11(10), 1007.
Mkindi AG, Tembo YLB, Mbega ER, Smith AK, Farrell IW, Ndakidemi PA, Stevenson PC, Belmain SR. 2020. Extracts of common pesticidal plants increase plant growth and yield in common bean plants. Plants 9, 149.
Mohana DC, Raveesha KA. 2007. Antifungal evaluation of some plant extracts against some plant pathogenic field and storage fungi. J Agric Technol 4(1), 119–137.
Mulugeta T, Muhinyuza JB, Gouws-Meyer R, Matsaunyane L, Andreasson E, Alexandersson E. 2020. Botanicals and plant strengtheners for potato and tomato cultivation in Africa. J Integr Agric 19(2), 406–427.
Mushobozi WL. 2010. Good agricultural practices on horticultural production for extension staff in Tanzania.
Mutayoba V, Ngaruko D. 2018. Assessing tomato farming and marketing among smallholders in high potential agricultural areas of Tanzania. Int J Econ Commer Manag 6(8), 577–590.
Mwelasi P. 2015. Analysis of phenolic compounds in Carica papaya, Zingiber officinale, Ipomoea batatas, and Myrothamnus flabellifolius using RP-HPLC-UV/VIS-DAD, and in vitro evaluation of antifungal activity on plant pathogenic fungi of economic importance. Midlands State University, Zimbabwe.
Nashwa SM, Abo-Elyousr KA. 2012. Evaluation of various plant extracts against the early blight disease of tomato plants under greenhouse and field conditions. Plant Prot Sci 48(2), 74–79.
Ngowi AVF, Mbise TJ, Ijani ASM, London L, Ajayi OC. 2007. Smallholder vegetable farmers in Northern Tanzania: Pesticides use practices, perceptions, cost and health effects. Crop Prot 26(11), 1617–1624.
Nisbet AJ. 2000. Azadirachtin from the neem tree (Azadirachta indica): Its action against insects. Anais da Sociedade Entomológica do Brasil 29, 615–632.
Nuwamanya AM, Runo S, Mwangi M. 2023. Farmers’ perceptions on tomato early blight, fungicide use factors and awareness of fungicide resistance: Insights from a field survey in Kenya. PLoS One 18(1), 0269035.
Patel CC, Singh D, Sridhar V, Choudhary A, Dindod A, Padaliya SR. 2019. Bioefficacy of cow urine and different types of bio-pesticide against major sucking insect pests of Bt cotton. Notes 105, 10–56.
Rahardjo IB, Marwoto B, Budiarto K. 2020. Efficacy of selected plant extracts to control leaf miner (Lyriomyza spp.) in chrysanthemum. AGRIVITA J Agric Sci 42(1), 37–44.
Raut JS, Karuppayil SM. 2014. A status review on the medicinal properties of essential oils. Ind Crops Prod 62, 250–264.
Raza W, Ghazanfar MU, Iftikhar Y, Ahmed KS, Haider N, Rasheed MH. 2016. Management of early blight of tomato through the use of plant extracts. Management 1(5), 1123–1133.
Roychoudhury R. 2016. Neem products. In: Ecofriendly Pest Management for Food Security, Academic Press, 545–562.
Saha R. 2016. Food fortification with tomato powder and subsequent analysis of physicochemical, nutritional and sensory properties.
Salazar Mendoza PS. 2023. Domestication, fertilization, and induced defences modulate tomato plant resistance to Tuta absoluta. Doctoral dissertation, Universidade de São Paulo.
Sasaki A, Itoh H, Gomi K, Ueguchi-Tanaka M, Ishiyama K, Kobayashi M, Matsuoka M. 2003. Accumulation of phosphorylated repressor for gibberellin signalling in an F-box mutant. Science 299(5614), 1896–1898.
Satyal P, Figueiredo AC, Setzer WN, Jäger AK. 2013. Chemical composition and antifungal activity of ginger (Zingiber officinale) essential oil against Alternaria solani and other plant pathogens. Int J Essent Oil Ther 7(2), 49–55.
Sheshma MK, Kakraliya SS, Dhanni D. 2022. Cost-benefit analysis of botanicals against leaf spot of mungbean (Vigna radiata L.) caused by Cercospora canescens. Pharm Innov J 11, 2175–2178.
Shuping DSS, Eloff JN. 2017. The use of plants to protect plants and food against fungal pathogens: A review. Afr J Tradit Complement Altern Med 14(4), 120–127.
Singh N, Yadav NK, Kumar A, Sangwan R. 2020. Effect of different dates of planting and weather parameters on early blight disease of tomato. Plant Dis Res 35(2), 127–131.
Singh UP, Maurya S, Singh A, Nath G, Singh M. 2012. Antimicrobial efficacy, disease inhibition and phenolic acid-inducing potential of chloroform fraction of cow urine. Arch Phytopathol Plant Prot 45(13), 1546–1557.
Sithanantham S, Seif AA, Ssennyonga J, Matoka C, Mutero C. 2002. Integrated pest management (IPM) issues in irrigated agriculture: Current initiatives and future needs to promote IPM adoption by smallholder farmers in Eastern Africa. The Changing Face of Irrigation in Kenya: Opportunities for Anticipating Changes in Eastern and Southern Africa, 231–261.
Sohrabi F, Solati Z, Bagheri F, Mirhosseini MA, Ziaee M, Pervez A. 2024. Insecticidal efficacy of synthesized ZnO nanoparticles using brown algae Cystoseira baccata extract against Callosobruchus maculatus (F.) (Col.: Chrysomelidae).
Spochacz M, Chowański S, Walkowiak‐Nowicka K, Szymczak M, Adamski Z. 2018. Plant‐derived substances used against beetles–pests of stored crops and food–and their mode of action: A review. Compr Rev Food Sci Food Saf 17(5), 1339–1366.
Sultana B, Anwar F, Przybylski R. 2007. Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam. trees. Food Chem 104(3), 1106–1114.
Tadele S, Emana G. 2017. Entomopathogenic effect of Beauveria bassiana (Bals.) and Metarhizium anisopliae (Metschn) on Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) larvae.
Tembo Y, Mkindi A, Mkenda P, Mpumi M, Mwanauta R, Stevenson PC, Ndakidemi P, Belmain SR. 2018. Pesticidal plant extracts improve yield and reduce insect pests on legume crops without harming beneficial arthropods. Front Plant Sci 9, 1425.
Tescari E, Chloridis A, Bacci L, Bradascio R, Giberti A. 2014. Spinetoram (Delegate®, Radiant®), a new insecticide with broad spectrum of activity.
The United Republic of Tanzania (URT). 2012. National Sample Census of Agriculture 2007/08 Regional Report: Morogoro Region. Tanzania.
Vaou N, Stavropoulou E, Voidarou C, Tsigalou C, Bezirtzoglou E. 2021. Towards advances in medicinal plant antimicrobial activity: A review study on challenges and future perspectives. Microorganisms 9(10), 2041.
Weber B, Halterman DA. 2012. Analysis of genetic and pathogenic variation of Alternaria solani from a potato production region. Eur J Plant Pathol 134, 847–858.
Wedge DE, Aiken RA, Simons AL. 2002. Antifungal activity of salannin and its possible mechanisms of action. J Agric Food Chem 50(6), 1550–1554.
Wonglom P, Daengsuwan W, Ito SI, Sunpapao A. 2019. Biological control of Sclerotium fruit rot of snake fruit and stem rot of lettuce by Trichoderma sp. T76-12/2 and the mechanisms involved. Physiol Mol Plant Pathol 107, 1–7.
Yanar Y, Gökçe A, Kadioglu I, Çam H, Whalon M. 2011. In vitro antifungal evaluation of various plant extracts against early blight disease (Alternaria solani) of potato. Afr J Biotechnol 10(42), 8291–8295.
Yoshida T, Iwasaki M, Yamaguchi S, Kato S. 2011. Shogaol from ginger exhibits potent antifungal activity against plant pathogens. Phytochemistry 72(7), 595–601.
Zafar S, Shah AA, Ashraf MA, Rasheed R, Muddasar M, Khan IM, Iqbal R. 2024. Plant growth under extreme climatic conditions. In: Environment, Climate, Plant and Vegetation Growth, Springer Nature, Cham, 133–178.
Zhao Y, Wang X, Zhang L, Wang K, Wu Y, Yao J, Chen Z. 2022. Anti-fungal activity of moutan cortex extracts against rice sheath blight (Rhizoctonia solani) and its action on the pathogen’s cell membrane. ACS Omega 7(50), 47048–47055.
Fikiri Abel, Angela Mkindi, Ernest Mbega, Philip C. Stevenson, Steven R. Belmain, Pavithravani Venkataramana (2025), Biopesticides potential to protect tomato (Solanum lycopersicum L.) production from early blight disease (Alternaria solani) and leaf miners (Tuta absoluta); IJB, V26, N2, February, P39-55
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