Bioremediation of gasoil by two indigenous bacterial strains in contaminated soils

Paper Details

Research Paper 01/11/2013
Views (314) Download (10)

Bioremediation of gasoil by two indigenous bacterial strains in contaminated soils

Sumayyah Najirad, Akbar Ghavidel, Hossein Ali Alikhani, Elnaz Sabbagh Taze, Seid said ghiasi, Leila Mohammadi
Int. J. Biosci.3( 11), 71-76, November 2013.
Certificate: IJB 2013 [Generate Certificate]


Some industrial activities may lead to hydrocarbon contaminations of soil. Bioremediation of these contaminants is more effective than the other physical-chemical remediation methods. Biological options are described by the term bioremediation, ‘the elimination, attenuation or transformation of polluting or contaminating substances by the use of biological processes, to minimize the risk to human health and the environment’ Therefore, the objective of this study was to investigate the efficiency of two autochthonous bacteria in bioremediation at optimal condition. These bacteria were isolated from oil-contaminated soils, southern Tehran refinery. The experimental section was carried out thorough three steps: (i) Isolation and purification of indigenous bacterial from contaminated soils of south Tehran refinery. (ii) Preparation of the soil, which was used as a medium for bioremediation. (iii) Determination of biological removal of gasoil from soil. Results indicated that in optimal environmental conditions (temperature, 27 ±2 °C, humidity of 60% WHC and daily aeration), bacterial isolates were able to degrade, about 93% of gasoil during the period of 45 days. This rate of removal seems to be acceptable, regarding to the short period of the experiments.


Ajayi AO, Balogun SA, Adegbehingbe K. 2008. Microorganisms in the crude oil-producing areas of Ondo State, Nigeria. Scientific Research and Essay 3(5), 174-179.

Alef K, Nannipieri P. 1995. Methods in applied soil microbiology and biochemistry: Academic Press.

Andrews JE, Brimblecombe P, Jickells TD, Liss PS, Reid B. 2013. An Introduction to Environmental Chemistry: Wiley.

Banks MK, Schwab P, Liu B, Kulakow PA, Smith JS, Kim R. 2003. The Effect of Plants on the Degradation and Toxicity of Petroleum Contaminants in Soil: A Field Assessment. In D. Tsao (Ed.), Phytoremediation (Vol. 78, 75-96 p). Springer Berlin Heidelberg.

Bento FM, Camargo FA, Okeke BC, Frankenberger WT. 2005. Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation. Bioresource technology 96(9),

Damborský J, Damborská M, Štípek S, Jesenská A, Trantírek L, Sklenár V. 2000. Effect of the carbon source on assessment of degrading bacteria with the spread-plating technique duringin situ bioremediation. Folia microbiologica 45(1), 35-40.

Devinny JS, Chang SH. 2000. Bioaugmentation for soil bioremediation. ENVIRONMENTAL SCIENCE AND POLLUTION CONTROL SERIES, 465-488.

Eaton AD, Franson MAH. 2005. Standard Methods for the Examination of Water & Wastewater: American Public Health Association.

Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA, Krieg NR, Phillips GB. 1981. Manual of methods for general bacteriology.

Idise OE, Ameh JB, Yakubu SE, Okuofu CA. 2010. Modification of Bacillus cereus and Pseudomonas aeruginosa isolated from a petroleum refining effluent for increased petroleum product degradation. African Journal of Biotechnology 9(22), 3303-3307.

Ilyina A, Castillo Sanchez MI, Villarreal Sanchez JA, Ramirez Esquivel G, Candelas Ramirez J. 2003. Isolation of soil bacteria for bioremediation of hydrocarbon contamination. Вecth. Мock. УН-ТА. СЕР 2, 88-91.

Márquez-Rocha F, Hernández-Rodrí V, Lamela M. 2001. Biodegradation of Diesel Oil in Soil by a Microbial Consortium. Water, Air, and Soil Pollution, 128(3-4), 313-320.

Mathew M, Tan LR, Su Q, Yang  X, Baxter M, Senior E. 2006. Bioremediation of 6% [w/w] Diesel- Contaminated Mainland Soil in Singapore: Comparison of Different Biostimulation and Bioaugmentation Treatments. Engineering in Life Sciences 6(1), 63-67.

Naji Rad S, Alikhani HA, Savaghebi Gh, Sabbagh Tazeh E, Ghavidel A. 2010. Bioremediation of oil contaminated soils by bacteria indigenous to oil-rich areas and the impact of environmental parameters. Ondokuz Mayıs University, Samsun, Turkey.

Okerentugba PO, Ezeronye OU. 2004. Petroleum degrading potentials of single and mixed microbial cultures isolated from rivers and refinery effluent in Nigeria. African Journal of Biotechnology 2(9), 288-292.

Page AL. 1982. Methods of soil analysis. Part 2. Chemical and microbiological properties. American Society of Agronomy, Soil Science Society of America.

USEPA. 2001. Guideline for the bioremediation of marine shorelines and fresh water wetland. Office of research and development, US Environmental Protection Agency.

Yang SZ, Jin HJ, Wei Z, He RX, Ji YJ, Li XM, Yu SP. 2009. Bioremediation of oil spills in cold environments: a review. Pedosphere, 19(3), 371-381.

Zhu   X, Venosa   AD, Suidan MT, Lee K. 2001. Guidelines for the bioremediation of marine shorelines and freshwater wetlands. US Environmental Protection Agency.