Biosynthesis of invertase by Penicillium chrysogenum using solid state fermentation technique
Paper Details
Biosynthesis of invertase by Penicillium chrysogenum using solid state fermentation technique
Abstract
Invertases are the enzymes which hydrolyze sucrose into D-glucose and D- fructose. The hydrolyzed products are the commercial carbohydrates. Invertases are of great industrial importance due to its applications in the cosmetics, feeding of the animals, detoxifying process of cassava, modification of natural polymers, synthesizing the organic chemicals, for decolorization of the ink from the paper to be recycled and the process for the wet grain milling. The present study is concerned with production of invertase from locally isolated Penicillium chrysogenum. The maximum production of invertase (11.97+0.03 U/ml/min) was obtained at incubation temperature of 50ºC growing on 10g of wheat bran after 72h of incubation when 1m of spore inoculum was used at pH 5 and 10 ml of the acetate buffer as moistening agent.
Abrams RA, Dobkin RS. 1994. Inhibition of return Effect of intentional cuing on eye movements. Journal of experimental psychology 3(20), 467-477.
Alberto F, Bignon C, Sulzenbacher G, Henrissat B, Czjzek M. 2004. The three-dimensional structure of invertase (β-fructosidase) from Thermotoga maritima reveals a bimodular arrangement and an evolutionary relationship between retaining and inverting glycosidases. The Journal of Biological Chemistry 279, 18903-18910.
Alegre ACP, Polizeli MLTM, Terenzi HF, Jorge IA, Guimarãe LHS. 2009. Production of thermostable invertases by Aspergillus caespitosus under submerged or solid state fermentation using agro-industrial residues as carbon source. Brazilian Journal of Microbiology 40, 612-622. http://dx.doi.org/10.1590/S151783822009000300025
Aranda C, Robledo A, Loera O, Contreras-esquivel JC, Rodriguez R, Aguilar CN. 2006. Fungal invertase expression in solid-state fermentation. Food Technology and Biotechnology 2(44), 229-233.
Ashok KB, Kayalvizhi N, Gunasekaran P. 2001. Optimization of media for â-fructofuranosidase production by Aspergillus niger in submerged and solid state fermentation. Process Biochemistry 37, 331-338.
Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry 72,248-254.
Benito MJ, Connerton IF, Córdoba JJ. 2006. Genetic characterization and expression of the novel fungal protease EPg222 active in dry-cured meat products. Applied Microbiology and Biotechnology 2(73), 356–365. http://dx.doi.org/10.1007/s00253-006-0498-z.
Bokosa I, Krastanov A, Roshkova ZI. 1992. Invertase biosynthesis by Saccharomyces cerevisiae. Nauchni TrJ 39, 269-279.
Chen WH, Liu CH. 1999. Production of β-fructofuranosidase by Aspergillus japonicas. Enzyme and Microbiology 2(18), 153-160.
Damle SP, Singh K, Ghosh D. 1958. Studies on soluble enzymes in Penicillin fermentation broth II. Invertase activity. Antibiotics Symposium, Council of Scientific and Industrial Research, New Delhi 137-144.
Gascon S, Neumann NP, Lampen JO. 1968. Comparative study of the properties of the purified internal and external invertases from yeast. Journal of Biological Chemistry 243, 1573–1577.
Giraldo MA, Silva TM, Salvato F, Terenzi HF, Jorge JA, Guimarães LHS. 2009. Thermostable invertases from Paecylomyces variotii produced under submerged and solid-state fermentation using agro-industrial residues. World Journal of Microbiology and Biotechnology 1-10. http://dx.doi.org/10.1007/s11274-011-0837-9.
Goosen C, Yuan XL, Munster JMV, Ram AFJ, Marc JEC, Van der Maarel MJEC, Dijkhuizen l. 2007. Molecular and Biochemical Characterization of a Novel Intracellular Invertase from Aspergillus niger with Transfructosylating Activity. Eukaryotic cell 4(6), 674-681.
Gomez SJR, Argur C, Viniegra-Gonzalez G. 2000. Invertase production by Aspergillus niger in submerged and solid state fermentation. Biotechnology Letter 22, 1255-1258.
Haq UI, Ali S, Aslam A, Qadeer MA. 2008. Characterization of a Saccharomyces cerevisiae mutant with enhanced production of beta-D-fructofuranosidase. Bioresource Technology 99, 7.
Kim BM. 1980. Studies on invertase from Kroean ginseng, Panax ginseng C. A. Meyer. Korean Journal of Food Science and Technology 12, 1-5.
Leitao AL. 2009. Potential of Penicillium Species in the Bioremediation Field. International Journal of Environmental Research and Public Health 6(4), 1393-1417. http://dx.doi.org/10.3390/ijerph6041393.
Kaur N, Sharma AD. 2005 Production optimization and characterization of extracellular invertase by an Actinomycete strain. Journal of Scientific and Industrial research 7(64), 515-519.
Neuro OM, Reyes F. 2002. Enzymes for animal feeding from Penicillium chrysogenum mycelial wastes from Penicillin Manufacture. Applied Microbiology 34, 413-416.
Obenland D, Simmen U, Boller T, Wiemken A.1993. Purification and characterization of three soluble invertase from barley leaves. Plant Physiology 101, 1331-1339.
Patil PR, Reddy GSN, Sulochana MB. 2011. Production, optimization and characterization of -fructofuranosidase by Chrysonilia sitophila PSSF84-A novel source. Indian journal of Biotechnology 10, 56-64.
Russo P, Garofalo A, Bencivenga U, Rossid S, Castagnoto A, Acunzo D, Gaeta FS, Mita DG. 1996. A non-isothermal bioreactor utilizing immobilized baker’s yeast cells. A study of the effect on invertase activity. Biotechnology and Applied Biochemistry 23, 141-148.
Rustigue lCB, Terenzi HF, Jorge JA, Guimarães LHS. 2010. Biochemical properties of an extracellular β-D-fructofuranosidase II produced by Aspergillus phoenicis under Solid-Sate Fermentation using soy bran as substrate. Electronic journal of Biotechnology 2(14), 2-10. http://dx.doi.org/10.2225.
Strum A, Hess D, Lee HS, Lienhard S. 2001. Neutral invertase is a novel type of sucrose-cleaving enzyme. Plant physiology 107, 159–165.
Sumner JB, Howell SF. 1935. A method for determination of saccharase activity. Journal of Biology and Chemistry 108, 51-54.
Shaheen I, Bhatti HN, Ashraf T. 2007. Purification and thermal characterization of invertase from a newly isolated Fusarium sp. under solid state fermentation. International Journal of Food Science and Technology 7(43), 1152-1158. http://dx.doi.org/10.1111/j.1365-2621.2007.01581.x
Sanchez MP, Huidobro JF, Mato I, Munigategui S, Sancho MT. 2001. Evolution of invertase activity in honey over two years. Journal of Agricultural Food Chemistry 49, 416-422.
Shafiq K, Ali S, Haq I. 2003. Time course study for yeast invertase production by submerged fermentation. Journal of Bacteriology 3, 984-988.
Uma C, Gomathi D, Muthulakshmi C, Gopalakrishnan VK. 2010. Production Purification and Characterization of Invertase by Aspergillus flavus using Fruit Peel Waste as Substrate. Advances in Biological Research 4(1), 31-36.
Zouaoui B, Ghalem BR, Fatima S, Djillali B. 2016. Optimization purification and characterization of invertase by Pseudomonas sp. isolated from the cane molasses.International Journal of Biosciences 9(1), 349-356. http://dx.doi.org/10.12692/ijb/9.1.349356
Shazia Malik, Tehreema Iftikhar, Asad Abbas, Hammad Majeed, Roheena Abdullah, 2016. Biosynthesis of invertase by Penicillium chrysogenum using solid state fermentation technique. Int. J. Biosci., 9(6), 330-337.
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