Biosynthesis of Streptomyces sp. bactericidal silver nanoparticles against antibiotic-resistant bacteria
Paper Details
Biosynthesis of Streptomyces sp. bactericidal silver nanoparticles against antibiotic-resistant bacteria
Abstract
The study investigated isolation and screening of antimicrobial producing Streptomyces sp. from soil sample and also in the biological synthesis of silver nanoparticles and their antimicrobial properties. Isolation of Streptomyces sp. from five different soil samples and about 10 different Streptomyces sp. isolates were separated and screened for the antimicrobial potentiality. From that the admirable isolates were selected based on the maximum diverse potential of antibacterial zone of inhibition. Furthermore, best isolate (ISB1) was used for the biological synthesis of silver nanoparticles and their synergistic antibacterial role is observed. Hence, this has been chosen for further optimization studies and biosynthesis of silver nanoparticles and confirmation of silver nanoparticles was carried by EDAX, size and morphology of the nanoparticles was determined by TEM and it was spherical; ranged between 20-50 nm. Synthesized nanoparticles were subjected to minimum inhibitory studies and 40 µl gave significant results compared to other concentrations. The biosynthesized silver nanoparticles by Streptomyces sp. showed better in killing pathogens that might be due to the synergistic effect. Thus, opens new platform for other application oriented studies.
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