Boswellia Carterii Gum Resin Induced Apoptosis on Human Colon Cancer Cell line HCT-116

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Research Paper 01/06/2018
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Boswellia Carterii Gum Resin Induced Apoptosis on Human Colon Cancer Cell line HCT-116

Hala M. Aldesouki, Germine M. Hamdy
Int. J. Biosci.12( 6), 308-322, June 2018.
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Colorectal cancer (CRC) represents one of the highest malignancies causing death globally. Although the cytotoxic and apoptotic-inducing effect of the chemotherapy-based drug,5-Fluorouracil (5-FU), its side effects are severe. Indeed, Boswellia carterii (B.C.) gum resin, with its active components possess anti-inflammatory and anti-cancer activities. This study aims to develop new natural-based anticancer drug via evaluating the cytotoxic, anti-proliferative and apoptotic effects of B.C. gum resin on the HCT-116 colon cancer cell line.B.C. gum resin active components were characterized using Gas Chromatography coupled Mass Spectrometry (GC/MS) and heavy metals analyses. The cytotoxic effect of B.C was evaluated via cell viability assay and morphological changes. Apoptotic-inducing effect of B.C. was assessed via caspase-3 activity and flow cytometry assay. Results revealed that the four terpenes; 24-Norursa-3,12-dien-11-one (56.53%), 24-Norursa-3,12-diene (20.66%), 24-Norursa-3,9(11),12-triene (11.278%) and 24-Noroleana-3,12-diene (7.678%) represents >96% of the total active B.C. components. Heavy metals results showed78% calcium, 18% magnesium with an excellent Selenium concentration. B.C. gum resin exhibited IC50 level of 1.74 ± 0.1 µg/ml accompanied with cell shrinking, organelles degradation and vacuoles formation in a concentration dependent manner. caspase-3 activity was significantly increased (p 0.03) by 3- and 4-fold in cells treated with 1.7 and 5 µg/ml from B.C. gum resin respectively, compared to the untreated cells. Flow cytometry analysis showed that cells treated with 1.7 and 5µg/ml of B.C. gum resin exhibited 78% and 84% of apoptotic cells respectively. In conclusion, B.C. gum resin is a promising natural therapeutic agent against CRC through its potent cytotoxic and apoptotic-inducing effects.


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