Cacao’s flair against colorectal despair: Elemental analysis, in vitro antioxidant and anticancer property of UF-18, Theobroma cacao powder against human colorectal cancer cells (HCT-116 Cells)
Paper Details
Cacao’s flair against colorectal despair: Elemental analysis, in vitro antioxidant and anticancer property of UF-18, Theobroma cacao powder against human colorectal cancer cells (HCT-116 Cells)
Abstract
The aim of the present study was to evaluate the safety, in vitro antioxidant and anti-cancer properties of UF-18 Theobroma cacao powder. The safety of cocoa powder was assessed by the elemental content through X-ray fluorescence spectroscopy. The antioxidant activity of the cocoa powder was evaluated using DPPH radical scavenging assay. The anticancer activity of cocoa powder was evaluated using the MTT assay against human colon cancer cells (HCT-116). Investigations showed that there was very low or trace amount of hеavy mеtal Cadmium (0.48 %) in grams. On thе othеr hand, potassium (K) has thе largеst contеnt (40.83%). Cacao powder also contained 33.1096 % of Calcium (Ca), 10.46% of Iron (Fe), 6.16% of Phosphorous (P), 4.55% Silicon (Si). Other metallic elements present in minute quantities include 0.002 % Nickel (Ni), 0.2 % Manganese (Mn) and 0.2 % Titanium (Ti). The DPPH radical scavenging assay showed that cacao powder exhibits strong antioxidant activity, with an IC50 value of 17.60 μg/mL. The MTT assay showed that cacao powder has moderately strong anticancer activity against HCT-116 cells, with an IC50 value of 15.81 µg/mL. Cacao powder is a safe and effective anti-cancer agent as investigated in vitro. Potential health benefits merit further investigation, particularly in vivo and human clinical trials of the UF-18 cacao powder as anticancer alternative.
Abu Bakar SA, Ali AM, Ahmad NH. 2019. Differential antiproliferative activity of goniothalamin against selected human cancer cell lines. Malaysian Journal of Medicine and Health Sciences 15(SUPP9), 66–73. Retrieved from https://medic.upm.edu.my/upload/dokumen/2019121815231010_MJMHS_0356.pdf
Acosta-Silva VJ, Olano-Martin DM, Ruiz-Ruiz MC. 2017. Chemical composition and in vitro anticancer activity of theobromine and caffeine from Theobroma cacao beans. Journal of Food Science and Technology 54(5), 1440–1446.
Ahmad N, Mukhtar H. 2013. The potential of cocoa polyphenols in cancer prevention. Biofactors 39(4), 303–310.
Alzahrani A, Al-Khalaf M. 2016. Anticancer activity of cocoa extracts and their constituents against human breast and colon cancer cell lines. Journal of Medicinal Food 19(12), 1494–1500.
Anand U, Dey A, Chandel AKS. 2022. Cancer chemotherapy and beyond: Current status, drug candidates, associated risks and progress in targeted therapeutics. Genes & diseases 10(4), 1367–1401. https://doi.org/10.1016/j.gendis.2022.02.007
Arozarena IE, Gamboa A. 2015. Evaluation of the antioxidant capacity and the in vitro anticancer activity of cocoa bean (Theobroma cacao L.) extracts. Food and Bioprocess Technology 8(5), 844–852.
Balti A, Han J, Van de Wouw M. 2014. Cocoa polyphenols and their potential health benefits. Current Opinion in Food Science 3, 42–51.
Beckett ST. 2014. Cocoa and chocolate health claims. Critical reviews in food science and nutrition 54(12), 1795–1802.
Carlsen L, Huntington KE, El-Deiry WS. 2022. Immunotherapy for colorectal cancer: Mechanisms and predictive biomarkers. Cancers 14(4), 1028. https://doi.org/10.3390/cancers14041028
Carrero JJ, Miller DR. 2005. Bioavailability of theobromine and caffeine in humans. Journal of Agricultural and Food Chemistry 53(9), 3581–3587.
Di Mattia CD, Sacchetti G, Mastrocola D, Serafini M. 2017. From cocoa to chocolate: The impact of processing on in vitro antioxidant activity and the effects of chocolate on antioxidant markers in vivo. Frontiers in Immunology 8, 1207. https://doi.org/10.3389/fimmu.2017.01207
Fatima N, Baqri SSR, Bhattacharya A. 2021. Role of flavonoids as epigenetic modulators in cancer prevention and therapy. Frontiers in Genetics 12, 758733. https://doi.org/10.3389/fgene.2021.758733
George VC, Kumar DR, Suresh PK, Kumar RA. 2015. Antioxidant, DNA protective efficacy and HPLC analysis of Annona muricata (soursop) extracts. Journal of Food Science and Technology 52(4), 2328–2335. https://doi.org/10.1007/s13197-014-1289-7
Jokhadze M, Eristavi L, Kutchukhidze J. 2007. In vitro cytotoxicity of some medicinal plants from Georgian Amaryllidaceae. Phytotherapy Research 21, 622–624.
Katz DL, Doughty K, Ali A. 2011. Cocoa and chocolate in human health and disease. Antioxidants & Redox Signaling 15(10), 2779–2811. https://doi.org/10.1089/ars.2010.3697
Khandelwal S, Sharma A, Sharma S. 2022. Antioxidant activity of soursop (Annona muricata L.) leaf extract and its phytochemical analysis. Food Chemistry 378, 131850.
Molyneux P. 2004. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. J Sci Technol 26(2), 211–219.
Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxic assays. Journal of Immunological Methods 65, 55–63.
Orak HH, Sevik Bahrisefi I, Sabudak T. 2019. Antioxidant activity of extracts of soursop (Annona muricata L.) leaves, fruit pulps, peels, and seeds. Pol J Food Nutr Sci 69(4), 359–366. DOI: 10.31883/pjfns/112654. http://journal.pan.olsztyn.pl
Ortega A, Munir S, Badshah SL. 2020. Important flavonoids and their role as a therapeutic agent. Molecules 25(22), 5243. https://doi.org/10.3390/molecules25225243
Pacubat RT, Magulod Jr G, Garcia JR. 2023. Exploring the pain-relieving power of a powder: In vitro cyclooxygenase I (COX) inhibitory activity of UF-18 cacao powder. DOI: 10.31838/ecb/2023.12.s3.792
Pacubat RT. 2022. Mineral and heavy metal analysis of banana bread added with cacao nibs. J Bio Env Sci 21(4), 81–94.
Pfeffer CM, Singh ATK. 2018. Apoptosis: A target for anticancer therapy. International Journal of Molecular Sciences 19(2), 448. https://doi.org/10.3390/ijms19020448
Satarug S, Garrett SH, Sens MA, Sens DA. 2010. Cadmium, environmental exposure, and health outcomes. Environmental Health Perspectives 118(2), 182–190. https://doi.org/10.1289/ehp.0901234
Schmitt MW, Loeb LA, Salk JJ. 2015. Colorectal cancer heterogeneity and targeted therapy: A case for molecular disease subtypes. Cancer Research 75(2), 245–249. DOI: 10.1158/0008-5472.CAN-14-1595
Singh BN, Shankar S, Srivastava RK. 2011. Green tea catechin, epigallocatechin-3-gallate (EGCG): Mechanisms, perspectives, and clinical applications. Biochemical Pharmacology 82(12), 1807–1821. https://doi.org/10.1016/j.bcp.2011.07.093
Sung H, Ferlay J, Siegel RL. 2021. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians 71(3), 209–249. https://doi.org/10.3322/caac.21660
World Health Organization. 2024. Colorectal cancer. Retrieved from https://www.who.int/news-room/fact-sheets/detail/colorectal-cancer
Zubaydah WOS, Sartinah A, Nuralifah. 2022. In vitro antioxidant activity of cocoa (Theobroma cacao L.) peel. https://www.myfoodresearch.com/uploads/8/4/8/5/84855864
Ronel T. Pacubat (2024), Cacao’s flair against colorectal despair: Elemental analysis, in vitro antioxidant and anticancer property of UF-18, Theobroma cacao powder against human colorectal cancer cells (HCT-116 Cells); JBES, V25, N4, October, P40-46
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