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Callus growth and plant regeneration in durum wheat (Triticum durum Desf.) immature embryos under abscisic acid (ABA) treatment

El Houssine Bouiamrine, Mohamed Diouri, Rachid EL Halimi, Lahcen Chillasse

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Int. J. Biosci.3(2), 87-98, February 2013

DOI: http://dx.doi.org/10.12692/ijb/3.2.87-98

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Abstract

The effect of abscisic acid (ABA) on callus growth and plant regeneration was studied in Four cultivars of durum wheat considered to have a good ability for in vitro culture. calluses induced from immature embryos on MS medium supplemented with 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) were transferred onto the same medium supplemented with different concentrations of ABA (0, 1, 2, 4 and 10 µM). The regeneration medium used was MS medium supplemented with 0.2 mg L-1 of 2,4-D, 10μM of benzylaminopurine (BAP) and 5μM of naphthaleneacetic acid (NAA). callus growth decreased with increasing concentration of ABA in the medium. Best regeneration rates were obtained from calli that were grown on media containing a low concentration of ABA (1 µM).The best number of plantlets per regenerating callus was also obtained from cultures on media containing 1μM ABA. Among the regenerated plants, some rare albino plants were obtained from calluses of Karim and Anouar varieties.

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Callus growth and plant regeneration in durum wheat (Triticum durum Desf.) immature embryos under abscisic acid (ABA) treatment

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