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Callus induction and plant regeneration from mature embryos of some Iranian wheat (Triticum aestivum L.) genotypes

Research Paper | May 1, 2017

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Shahnam Azizi Dargahlou, Ebrahim Dorani Uliaie, Ali Bandehagh

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J. Bio. Env. Sci.10( 5), 275-283, May 2017


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Wheat is one of the most widely planted crop across the world. A simple, efficient and credible in-vitro culture is necessary for genetic improvement of wheat. In this study we evaluated the effect of genotype and different combination and concentration of PGRs and sugar content as carbon source on callus induction and plant regeneration from mature embryos. To do so, the embryos were transferred to MS medium supplemented with 2 mg/L 2,4-D for callus induction; the calli were then transferred to basal MS medium without PGRs for plant regeneration. Analysis of variance showed significant difference (P<0.01) among the genotype son callus induction, callus weight, and shoot regeneration. Callus induction were observed in all genotypes and percentage of callus induction ranged from 60 to 100%. The highest percentage of shoot regeneration obtained in the Aflak (%26) in PGRs-free basal MS medium. Medium containing BAP in combination with a week auxin including IAA and IBA, showed high regeneration capacity compared to media supplemented with BAP (0.5 and 2mg/L). Medium supplemented with 2mg/L BAP along with 0.5mg/L IAA showed the highest percentage of shoot regeneration (37.5%). Using 15 and 30g/L sugars in PGRs-free 1/2 MS basal medium did not show significant influence on shoot regeneration whereas these media displayed better shoot regeneration in compare to medium containing 0.5mg/L BAP. Aseptic culture is an indispensable basis for genetic engineering of plants therefore results of this study can be used in genetic transformation of wheat to improve agronomic traits.


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Callus induction and plant regeneration from mature embryos of some Iranian wheat (Triticum aestivum L.) genotypes

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