Paper Details

Callus  induction on  Jasminum sambac  L.  by  2,4-dichlorophenoxy acetic acid hormone

Bibi Latifeh Davallo, Seyed Kamal Kazemitabar, Abbas Gholipour, Sina Ghanbari

DOI: http://dx.doi.org/10.12692/ijb/5.2.114-118

Int. J. Biosci. 5(2), 114-118. July, 2014. (PDF)

Abstract:

Jasminum sambac (L.) Aiton (Family: Oleaceae), an ornamental plant extensively used in perfumery and religious purposes, is a herb which shows shrub-like appearance after two years. It is locally known as ‘Motia’ and produces white flowers with a very pleasant fragrance. In recent years, plant tissue culture techniques has become a powerful tool for the propagation of many plant species. In this research weight and diameter of callus were analyzed to determination of appropriate medium culture. This study were performed on spring of 2014 in complete randomized design with three replicated. The MS medium culture was contain 2,4-D hormone. ANOVA statistical analysis showed significant difference at 1% level. Highest callus diameter belong to medium contain 0.3 mg/l 2,4-D , also maximum callus weight belong to 0.3 mg/l 2,4-D. Therefore, hormones amount which used in this research can induced callus in sesame.