Carbohydrate source and concentration affect in vitro growth indices of the selected wild cherry (Prunus avium L.) genotype
Paper Details
Carbohydrate source and concentration affect in vitro growth indices of the selected wild cherry (Prunus avium L.) genotype
Abstract
In vitro shoots of the selected genotype of wild cherry grown in natural forests of Nour (north forests of Iran ) were subcultured in Murashige and Skoog medium (1/2 N) containing 1 mgl-1 BA and 0.1 mgl-1 IBA and three different source of carbohydrates (Sucrose, Glucose and fructose) at three levels (0, 15, 30 and 45 g l-1 ) for proliferation stage. Mean height of longest shoot, Coefficient of multiplication, as the mean of new shoots formed per micro cutting in each subculture, mean of leaf number, dry weight, minerals percentage (N, P, K and Ca) and sucrose content were measured in shoots after 30 and 45 days. Fructose resulted as the best source of carbohydrate inducing superior rates of growth indices. Higher concentrations, more than 30 g l-1, of each carbohydrate improved the results. The highest sucrose content in shoots was achieved in media supplemented with 45 gl-1 sucrose and fructose after 15 and 30 days, respectively. The maximum uptake of N (%) was achieved in shoots treated by 45 g l-1 fructose both after 15 and 30 days. It was concluded that type of carbon source affected significantly growth indices, consequently the rate of mineral absorption and levels of carbohydrates of the in vitro shoots at different stages of growth.
Akita M, Negishi K, Kitano A, Iwasaki M, Komae R, Ohta Y, Kuriu T, Takii T. 2006. Mass propagation of cherry (Cerasus×yedoensis MATSUM.) through shoot primordia. Acta Horticulture 725, 579-584.
Baskaran P, Jayabalan N. 2005. Role of basal media, carbon sources and growth regulators in micropropagation of Eclipta alba a valuable medicinal herb. KMITL Science Journal 5, 469-482.
Bidarigh S, Azarpour E. 2011. Evaluation effect of temperature management on rooting in micro cutting of poinsettia. World Applied Science Journal 14, 654-657.
Bozena B, Szczerba J. 1991. Influence of different carbon sources on invertase activity and growth of sour cherry (Prunus cerasus L.) shoot cultures. Journal Experimental Botany 42, 911-915. http://dx.doi.org/ 10.1093/jxb/42.7.911
Brink RH, Dubar P, Lynch D L. 1960. Measurement of carbohydrates in soil hydrolysates with anthrone. Soil Science 89, 157–166. http://dx.doi.org/10.1097/00010694-196003000-00006
Devaux C, Baldet P, Joubès J, Dieuaide-Noubhani M, Just D, Chevalier C, Raymond P. 2003. Physiological, biochemical and molecular analysis of sugar-starvation responses in tomato roots. Journal of Experimental Botany 54, 1143-1151. http://dx.doi.org/10.1093/jxb/erg113
Ďurkovič J. 2006. Rapid micropropagation of mature wild cherry. Biologia Plantarum. 50, 733-736. http://dx.doi.org/10.1007/s10535-006-0118-x
Eapen S, Georg L. 1990. Influence of phytohormones, carbohydrates, amino acids, growth supplements and antibiotics on somatic embryogenesis and plant differentiation in finger millet. Plant Cell Tissue Organ Culture 22, 87- 93. http://dx.doi.org/10.1007/BF00043683
Emanuel E, Bloom AJ. 2005. Mineral nutrition of plants: principles and perspectives. 2 edition, Academic Press. p. 147.
Fuentes G, Talavera C, Desjardins Y, Santamaría JM. 2007. Low exogenous sucrose improves ex vitro growth and photosynthesis in coconut in vitro plantlets if grown in vitro under high light. Acta Horticulture 748, 151-155.
Grant NJ, Hammatt N. 1999. Increased root and shoot production during micropropagation of cherry and apple rootstocks: effect of subculture frequency. Tree Physiology 19, 899–903.
Hajnajari H, Hasanloo T, Asghary AH, Izadpanah M. 2009. Influence of different nitrogen sources on in vitro shoot’s growth characteristics of the selected genotype of wild cherry (Prunus avium L.). Seed and Plant 24, 749-762.
Hammatt N, Grant NJ. 1996. Micropropagation of mature British wild cherry. Plant Cell Tissue Organ Culture 47, 103-110. http://dx.doi.org/10.1007/BF02318945
Harada H, Murai Y. 1996. Micropropagation of Prunus mume. Plant Cell Tissue Organ Culture 46, 265–267. http://dx.doi.org/ 10.1007/BF02307104
Hasanloo T, Hajnajjari H, Fahimi H, Nasiri M. 2006. Evaluation of ionic equilibrium and propagation coefficient of jojoba (Simmondsia chinensis (LINK) SCH.) in vitro. Iranian Journal of Rangelands and forests plant breeding 14, 105-113.
Ivanica J. 1992. Micropropagation of cherry in: Bajaj YPS (ed). Biotechnology in agriculture and forestry. 18. Spriger- Verlag.
Kunst A, Draeger B, Ziegenhorn J. 1984. Colorimetric methods with glucose oxydase and peroxydase. In: Bergmeyer HU., (ed). Methods of enzymatic analysis 6, 178-185. Verlag chemie, weinheim.
Leifert C, Pryce S, Lumsden PJ, Woutes WM. 1992. Effect of medium acidity on growth and rooting of different plants growing in vitro. Plant Cell Tissue Organ Culture 30, 171- 179. http://dx.doi.org/10.1007/BF00040019
Madhulatha P, Kirubakaran SI, Sakthivel N. 2006. Effects of carbon sources and auxins on in vitro propagation of banana. Biologia Plantarum 50, 782-784. http://dx.doi.org/10.1007/s10535-006-0131-0
Mclachland KD. 1976. Comparative phosphorus responsive plants to the changes of variable phosphorus situations. Australian Journal of Agriculture Research 27, 323- 341.
Miller BD, Timmer VR. 1997. Nutrient dynamics and carbon partitioning in nutrient loaded Picea mariana [Mill.] B.S.P. Seedlings during Hardening. Scandinavian Journal of Forest Research 12, 122-129. http://dx.doi.org/10.1080/02827589709355393
Nigra H..M, Alvarez MA, Giulietti AM. 1990. Effect of carbon and nitrogen sources on growth and solasodine production in batch suspension cultures of Solanum eleagnifolium cav. Plant Cell Tissue Organ Culture 21, 55-60. http://dx.doi.org/10.1007/BF00034492
Pritchard J, Wyn-Jones RG, Tomos AD. 1991. Turgor, growth and rheological gradients in wheat roots following osmotic stress. Journal of Experimental Botany 42, 1043-1049. http://dx.doi.org/10.1093/jxb/42.8.1043
Ružić DV, Vujović TI. 2008. The effects of cytokinin types and their concentration on in vitro multiplication of sweet cherry cv. Lapins (Prunus avium L.). Horticultural Science (Prague) 35, 12–21.
Ružić D, Sarić M, Cerović R, Ćulafić L. 2000. Relationship between the concentration of macroelements, their uptake and multiplication of cherry rootstock Gisela 5 in vitro. Plant Cell Tissue Organ Culture 63, 9-14. http://dx.doi.org/10.1023/A:1006412901992
Sabeti H 1995. Forests, trees and bushes of Iran. Tehran, Iran, Ministry of Information and Tourism Press. p. 418–9.
Schmidt H, Ketzel A. 1996. In vitro culture techniques in sweet cherry breeding. Acta Horticulturae 410, 111-114
Dussert S, Verdeil JL, Rival A, Noirot M, Buffard-Morel J.1995. Nutrient uptake and growth of in vitro coconut (Cocos nusifera L.) calluses. Plant Science 106, 185- 93. http://dx.doi.org/10.1016/0168-9452(95)04079-A
Stromberger JA, Tsai GY. 1994. Interaction of potassium with nitrogen and their influence on growth and yield potential in maize. Plant Nutrition 17, 19-37. http://dx.doi.org/10.1080/01904169409364707
Troyanos YE, Hipps NA, Moorby J, Ridout MS. 1997. The effects of external magnesium concentration on the growth and magnesium inflow rates of micropropagated cherry rootstocks F.12/1′ (Prunus avium L.) and `Colt’ (Prunus avium L.×Prunus pseudocerasus L.). Plant Soil 197, 25-33. http://dx.doi.org/10.1023/A:1004264806741
Tuija Aronen, T, Pehkonen T, Ryynänen L. 2009. Enhancement of somatic embryogenesis from immature zygotic embryos of Pinus sylvestris, Scandinavian Journal of Forest Research 24, 372-383. http://dx.doi.org/10.1080/02827580903228862
Zilkah S, Faingersh E, Rotbaum A. 1992. In vitro propagation of three MXM (Prunus avium×Prunus Mahaleb) cherry rootstocks. Acta Horticulturae 314, 93-98.
Hassan Hajnajari, Tahereh Hasanloo (2013), Carbohydrate source and concentration affect in vitro growth indices of the selected wild cherry (Prunus avium L.) genotype; IJB, V3, N9, September, P214-221
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