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Carbohydrate source and concentration affect in vitro growth indices of the selected wild cherry (Prunus avium L.) genotype

Hassan Hajnajari, Tahereh Hasanloo

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Int. J. Biosci.3(8), 174-181, August 2013

DOI: http://dx.doi.org/10.12692/ijb/3.8.174-181

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Abstract

In vitro shoots of the selected genotype of wild cherry grown in natural forests of Nour (north forests of Iran) were subcultured in Murashige and Skoog medium (1/2 N) containing 1 mgl-1 BA and 0.1 mgl-1 IBA and three different source of carbohydrates (Sucrose, Glucose and fructose) at three levels (0, 15, 30 and 45 g l-1 ) for proliferation stage. Mean height of longest shoot, Coefficient of multiplication, as the mean of new shoots formed per micro cutting in each subculture, mean of leaf number, dry weight, minerals percentage (N, P, K and Ca) and sucrose content were measured in shoots after 30 and 45 days. Fructose resulted as the best source of carbohydrate inducing superior rates of growth indices. Higher concentrations, more than 30 g l-1, of each carbohydrate improved the results. The highest sucrose content in shoots was achieved in media supplemented with 45 gl-1 sucrose and fructose after 15 and 30 days, respectively. The maximum uptake of N (%) was achieved in shoots treated by 45 g l-1 fructose both after 15 and 30 days. It was concluded that type of carbon source affected significantly growth indices, consequently the rate of mineral absorption and levels of carbohydrates of the in vitro shoots at different stages of growth.

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