Cell sensitivity assay and cell viability of Breast cancer cell (MCF7) using MTT assay of Ashitaba (Angelica Keiskei) leaf extract

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Research Paper 15/11/2022
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Cell sensitivity assay and cell viability of Breast cancer cell (MCF7) using MTT assay of Ashitaba (Angelica Keiskei) leaf extract

Justin V. Dumale, Geraldine R. Gamoso
Int. J. Biosci.21( 5), 223-226, November 2022.
Certificate: IJB 2022 [Generate Certificate]

Abstract

The 3-(4,5-dimethylethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay was used to investigate Angelica keiskei Koiz mi (Ashitaba in Japanese), a perennial plant belonging to the Umbelliferae family, for its cytotoxic effect against breast cancer cells or MCF, to an insoluble purple formazan by the action of mitochondrial reductase. Formazan is then solubilized and the concentration determined by optical density at 570nm. The plant extract is not cytotoxic to breast cancer cells and has no impact even at high concentrations. These findings indicate that the plant extract has a detrimental effect on the cell. Furthermore, prior to testing, the components of the plant extract must be extracted.

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