Changes in acid and alkaline phosphatase enzyme activity in rhizosphere ash Fraxinus rotundifolia and its correlation with soil and plant phosporus

Paper Details

Research Paper 01/05/2014
Views (153) Download (6)

Changes in acid and alkaline phosphatase enzyme activity in rhizosphere ash Fraxinus rotundifolia and its correlation with soil and plant phosporus

Bahman Zamani Kebrabadi, Mohammad Matinizadeh, MehrdadGhodskhah Daryayi, Ali Salehi
J. Bio. Env. Sci.4( 5), 233-238, May 2014.
Certificate: JBES 2014 [Generate Certificate]


We investigated in field experiments seasonal dynamics of acid and alkaline phosphatase activity. Alkaline and acid phosphatase activity showed a seasonal pattern. Alkaline phosphatase activity was significantly higher in springandacid phosphatase activity significantly higherin autumn.Soil phosphorus activity in spring is considerably higher than soil phosphorus activity in the autumn.The results showed that average plant phosphorusin spring higher than the autumn but difference between average plant phosphorus on the treatments (spring and autumn) was not significant. Average plant phosphorusin spring higher than the autumn but difference between average plant phosphorus on the treatments (spring and autumn) was not significant. The results of correlation analysis indicated that there wassignificant correlation among alkaline phosphatase with available phosphorus.


Bedini S, Turrini A, Rigo C, Argese E and Giovannetti M. 2010. Molecular charac-terization and glomalin production of arbuscularmycorrhizal fungi colonizinga heavy metal polluted ash disposal island, downtown Venice. Soil BioBiochem 42, 758– 765.

Bolan NS. 1991. A critical review on the role of mycorrhizal fungi in the uptake of phosphorus by plants. Plant Soil 134, 189–207.

Clark RB, and Zeto SK. 2000.Mineral acquisition by arbuscular mycorrhizal plants. Journal of Plant Nutr 23, 867–902.

Cozzolino V, Pigna M, Di Meo V, Caporale AG and Violante A. 2010. Effects of arbuscular mycorrhizal inoculation and phosphorus supply on the growth of Lactuca sativa L. and arsenic and phosphorus availability in an arsenic polluted soil under non-sterile conditions. Applied Soil Ecology 45, 262–268.

Dodd JC, Burton, CC, Burns RG, and Jeffries P. 1987. Phosphatase activity associatedwith the roots and the rhizosphere of plants infected with vesicular-arbuscularmycorrhizal fungi. New Phytologist 107, 163–172.

Gee GW and Bauder JW. 1982. Particle size analysis. In: Klute, A. (Ed.), Methods of Soil Analysis, Part 1 Physical and Mineralogical Methods. ASA Monograph number 9. Madison, WI, USA, 383–411.

Huang W, Liu j, Zhou G, Zhang D and DengQ. 2011. Effects of precipitation on soil acid phosphatase activity in three successional forests in southern China. Biogeosciencis 8, 1901-1910.

Javot H, Pumplin N, and Harrison MJ. 2007. Phosphate in the arbuscular mycorrhizalsymbiosis: transport properties and regulatory roles. Plant. Cell Environ 30, 310–322.

Kothari SK, Marschner H and Römheld V. 1990. Direct and indirect effects of VAmycorrhizal fungi and rhizosphere microorganisms on acquisition of mineralnutrients by maize (Zea mays L.) in a calcareous soil. New Phytologist 116, 637–645.

Krämer S, and Green DM. 2000. Acid and alkaline phosphatase dynamics and their relationship to soil microclimate in a semiarid woodland. Soil Biology and Biochemistry 32, 179-188.

Li X, Marschner H, and George E. 1991. Acquisition of phosphorus and copper by VA-mycorrhizal hyphae and root-to-shoot transport in white clover. Plant Soil 136, 49–57.

Mar Vázquez M, César S, Azcón R and Barea JM. 2000. Interactions between arbuscular mycorrhizal fungi and other microbial inoculants (Azospirillum, Pseudomonas, Trichoderma) and their effects on microbial population and enzyme activities inthe rhizosphere of maize plants. Applied Soil Ecology 15, 261–272.

Miller R, and Jastrow J. 1990).Hierarchy of root and mycorrhizal fungal interactionswith soil aggregation. Soil Biology & Biochemistry 22, 579– 584.

Nakas JP, Gould WD, and Klein DA. 1987. Origin and expression of phosphatase activity in a semi-arid grassland soil. Soil Biology & Biochemistry 19, 13-18.

Richardson AE, Hocking PJ, Simpson RJ, and George TS. 2009. Plant mechanisms tooptimise access to soil phosphorus. Crop & Pasture Science 60, 124–143.

Sanders FE, Tinker PB. 1973. Phosphate flow into mycorrhizal roots. Pesticide Science 4, 385–395.

Sanyal SK, and De Datta SK. 1991. Chemistry of phosphorus transformations in soil. Advances in Soil Science 16, 1–120.

Skujins J . 1976 Extracellular enzymes in soil.CRC Crit. Rev. Microbiol 4, 383–421.

Smith SE, Gianinazzi-Pearson V, Koide RandCairneyJWG. 1994. Nutrient trans-port in mycorrhizas: structure, physiology and consequences for efficiency ofthe symbiosis. Plant Soil 159, 103– 113.

Smith SE, and Read DJ. 2008. Mycorrhizal Symbiosis. Academic Press Ltd., London.

Smith SE, JakobsenI, Grönlund M, and Smith FA. 2011. Roles of arbuscularmycorrhizas in plant phosphorus nutrition: interactions between pathways ofphosphorus uptake in arbuscular mycorrhizal roots have important implications for understanding and manipulating plant phosphorus acquisition. Plant Physiology 156, 1050–1057.

Speir TW, and Cowling JC. 1991. Phosphatase activities of pasture plants and soils: relationship with plant productivity and soil P fertility indices. Biology and Fertility of Soils 12, 189-194.

Speir TW and Ross DJ. 1978. Soil phosphatase and sulphatase. In: Burns, R.G. (Ed.), Soil Enzymes. Academic Press, London, 197-215.

Tarafdar JC, and Chhonkar PK. 1979. Phosphatase production by microorganisms isolated from diverse types of soils. Zentralblattfuer Bakteriologie 134, 119-124.

Tarafdar JC, and Claassen N. 1988. Organic phosphorus compounds as a phosphorussource for higher plants through the activity of phosphatases produced by plantroots and microorganisms. Biology and Fertility of Soils 5, 308–312.

Van Aarle IM, and Olsson PA. 2008. Resource partitioning between extraradical and intraradical AM. Fungal mycelium. In: Varma, A. (Ed.), Mycorrhiza: State of the Art, Genetics and Molecular Biology, Eco-Function, Biotechnology, Eco-Physiology, Structure and Systematics, third ed. Springer, Heildeberg, 321–336.

Marschner H. 1995. Mineral Nutrition of Higher Plants, second ed. Academic Press, Boston.

Olsen, S.L., Sommers, L.E., 1982. Phosphorus. In: Page, A.L., Miller, R.H., Keeney, D.R. (Eds.), Methods of Soil Analysis. Part 2: Chemical and Microbiological Properties. , 2nd ed. American Society of Agronomy, Madison, 403–427.

Tabatabai MA, and Bremner JM. 1969. Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biology & Biochemistry 1, 301–307.

WalingI, Vanvark W, Houba VJ, Gand Vanderlee JJ. 1989. Soil and plant analysis, a series of syllabi, part 7, plant analysis procedures.Wageningen Agricultural University