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Changes in activity profile of superoxide dismutase in barley cultivars seedling under salt stress

Research Paper | July 1, 2015

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Mohammad Behrouzi, Mostafa Valizadeh, Mohammad Moghaddam Vahed

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J. Bio. Env. Sci.7( 1), 350-357, July 2015


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Salt stress is one of the most important factors limiting barley cultivation in arid and semi arid regions. Meanwhile, salt induced production of reactive oxygen species (ROS) induces the activity of some antioxidant enzymes in order to protect plants against stress condition. This study was aimed to investigate the activity of superoxide dismutase (SOD) in ten Iranian indigenious barley cultivars in the seedling stage. A factorial experiment was conducted using three NaCl levels (0, 100 and 200 mM), in combination with three levels of proline (0, 5 and 10 mM) based on a completely randomized design with three replications. Seedlings shoots in each plot were mixed-harvested. Electrophoretic analyses were performed by using 8% slab polyacrylamide gels. Obtained results for three detected SOD isozymes activities revealed that the difference between barley cultivars were significant. Interaction of salinity × cultivar was also significant for SOD2. In general, cultivar Torsh due to having more SOD activity could be introduced as the tolerant cultivar to salinity. Finally, it suggests an antioxidant analysis by gel electrophoresis as a useful tool for studying plant’s tolerance to salt stress.


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Changes in activity profile of superoxide dismutase in barley cultivars seedling under salt stress

Ahmed IM, Dai H, Zheng W, Cao F, Zhang G, Sun D, Wu F. 2013. Genotypic differences in physiological characteristics in the tolerance to drought and salinity combined stress between Tibetan wild and cultivated barley. Plant Physiology and Biochemistry 63, 49- 60.

Alscher RG, Erturk N, Heath LS. 2002. Role of superoxide dismutases (SOD) in controlling oxidative stress in plant. Journal of Experimental Botany 153, 1331- 1341.

Apel K, Hirt H. 2004. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annual Review in Plant Biology 55, 373- 399.

Bakalova S, Nikolova A, Nedeva D. 2004. Isoenzme profiles of peroxidase, catalase and superoxide dismutase affected by dehydration stress and ABA during germination of wheat seeds. Bulgarian Journal of Plant Physiology 30, 64- 77.

Bartels D, Sunkar R. 2005. Drought and salttolerance in plants. Critical Reviewe in Plant Science 24, 23- 58.

Choudhary  NL,  Sairam  RK,  Tyagi  A.  2005. Expression of delta1-pyrroline-5-carboxylate synthetase gene during drought in rice (Oryza sativa L.). Indian Journal of Biochemistry and Biophysics 42, 366– 370.

Del Rio LA, Sandalio LM, Corpas FJ, Palma JMBarroso JB. 2006. Reactive oxygen species and reactive nitrogen species in peroxisomes. Production, scavenging, and role in cell signaling, Plant Physiology 141, 330- 335.

Garthwaite A, Bothmer R, Colmer, T. 2005. Salt tolerance inwild Hordeum species is associated with restricted entry of Na+ and Cl− into the shoots. Journal of Experimental Botany 56, 2365– 2378.

Gill SS, Tuteja N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48, 909- 930.

Hasegawa PM, Bressnan RA, Zhu JK, Bohnert HJ. 2000. Plant cellular and molecular responses to high salinity. Annual Review of Plant Physiology and Plant Molecular Biology 51, 463– 499.

Hossain MA, Fujita M. 2010. Evidence for a role of exogenous glycinebetaine and proline in antioxidant defense and methylglyoxal detoxification systems in mung bean seedlings under salt stress. Physiology and Molecular Biology of Plants 16, 19-29.

Hossain MA, Hasanuzzaman M, Fujita M. 2011. Coordinate induction of antioxidant defense and glyoxalase system by exogenous proline and glycinebetaine is correlated with salt tolerance in mung bean. Frontiers of Agriculture in China 5, 1– 14.

Kartashov AV, Radyukina NL, Ivanov Yu V, Pashkovskii PP, Shevyakova NI, Kuznetsov VV. 2008. Role of antioxidant systems in wild plant adaptation to salt stress. Russian Journal of Plant Physiology 55, 463– 468.

Manchania AM, Banks SW, Gossett R, Bellaire BA, Lucas MC, Millhollon EP. 1999. The influence of alpha-amanitin on NaCl induced up-regulation of antioxidant enzyme activity in cotton callus tissue. Free Radical Research 30, 429- 438.

Mittler R, Vanderauwera S, Gollery M, Breusegem F. 2004. Reactive oxygen gene network of plants. Trends in Plant Science 9, 490– 498.

Mourato M, Reis R, Martins LL. 2012. Characterization of Plant Antioxidative System in Response to Abiotic Stresses: A Focus on Heavy Metal Toxicity. In: Dr. Montanaro G, Ed. Advances in Selected Plant Physiology Aspects.

Munns R, Tester M. 2008. Mechanisms of salinity tolerance. Annual Review in Plant Biology 59, 651-681.

Nounjana N, Nghiab PT, Theerakulpisut P. 2012. Exogenous proline and trehalose promote recovery of rice seedlings from salt-stress and differentially modulate antioxidant enzymes and expression of related genes. Journal of Plant Physiology 169, 596- 604.

Panda SK, Khan MH. 2004. Changes in growth and superoxide dismutase activity in Hydrilla verticillata L. under abiotic stress. Brazillian Journal of Plant Physiology 16, 115- 118.

Pastori GM, Foyer CH. 2002. Common components, networks, and pathways of cross-tolerance to stress. The central role of “Redox” and abscisic acid-mediated controls. Plant Physiology 129, 460– 468.

Poulik LMD. 1957. Starch gel electrophoresis in discontinuous system of buffers. Nature 180, 1477-1485.

Radyukina NL, Shashukova AV, Shevyakova NI, Kuznetsov VV. 2008. Proline involvement in the common sage antioxidant system in the presence of NaCl and paraquat. Russian Journal of Plant Physiology 5, 649– 656.

Rhoads DM, Umbach AL, Subbaiah CC, Siedow JN. 2006. Mitochondrial reactive oxygen species: Contribution to oxidative stress and interorganellar signaling. Plant Physiology 141, 357-366.

Salek jalali M, Haddad R, Jafari B. 2012. Effect of soil water shortages on the activity of antioxidant enzymes and the contents of chlorophyllls and proteins in barley. Journal of Agricultural and Environmental Science 1, 57- 63.

Scandalios JG. 2005. Oxidative stress: Molecular perception and transduction of signals triggering antioxidant gene defenses. Brazilian Journal of Medical and Biological Research 38, 995- 1014.

Seckin B, Turkan I, Sekmen AH, Ozfidan C. 2010. The role of antioxidant defense systems at differential salt tolerance of Hordeum marinum Huds. (sea barleygrass) and Hordeum vulgare L. (cultivated barley). Environmental and Experimental Botany 69, 76– 85.

Shannon MC. 1997. Adaptation of plants to salinity. Advances in Agronomy 60, 75– 120.

Smirnoff N, Cumbes QJ. 1989. Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 28, 1057- 1060.

Soltis DE, Soltis PS. 1990. Isozymes in Plant Biology. Chpman and Hall. London, P. 259.

Soshinkova TN, Radyukina NL, Korolkova DV, Nosov AV. 2013. Proline and functioning of the antioxidant system in Thellungiella salsuginea plants and cultured cells subjected to oxidative stress. Russian Journal of Plant Physiology 60, 41- 54.

Szabados L, Savouré A. 2009. Proline: a multifunctional amino acid. Trends in Plant Science 15, 89– 97.

Valizadeh M, Mohayeji M, Yasinzadeh N, Nasrullazadeh S, Moghaddam M. 2011. Genetic Diversity of Synthetic Alfalfa Generations and Cultivars Using Tetrasomic Inherited Allozyme Markers. Journal of Agriculture Science and Technology 13, 425- 430.

Yoshiba Y, Kiyosue T, Katagiri T, Ueda H, Mizoguchi T, Yamaguchi-Shinozaki K, Wada K, Harada Y, Shinozaki K. 1995. Correlation between the induction of a gene for delta 1-pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress. Plant Journal 7, 751– 760.

Zhao GQ, Ma BL, Ren CZ. 2007. Growth, gas exchange, chlorophyll fluorescence, and ion content of naked oat in response to salinity. Crop Science 47, 123– 131.