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Research Paper | July 1, 2015

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Changes in activity profile of superoxide dismutase in barley cultivars seedling under salt stress

Mohammad Behrouzi, Mostafa Valizadeh, Mohammad Moghaddam Vahed

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J. Bio. Env. Sci.7(1), 350-357, July 2015

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Abstract

Salt stress is one of the most important factors limiting barley cultivation in arid and semi arid regions. Meanwhile, salt induced production of reactive oxygen species (ROS) induces the activity of some antioxidant enzymes in order to protect plants against stress condition. This study was aimed to investigate the activity of superoxide dismutase (SOD) in ten Iranian indigenious barley cultivars in the seedling stage. A factorial experiment was conducted using three NaCl levels (0, 100 and 200 mM), in combination with three levels of proline (0, 5 and 10 mM) based on a completely randomized design with three replications. Seedlings shoots in each plot were mixed-harvested. Electrophoretic analyses were performed by using 8% slab polyacrylamide gels. Obtained results for three detected SOD isozymes activities revealed that the difference between barley cultivars were significant. Interaction of salinity × cultivar was also significant for SOD2. In general, cultivar Torsh due to having more SOD activity could be introduced as the tolerant cultivar to salinity. Finally, it suggests an antioxidant analysis by gel electrophoresis as a useful tool for studying plant’s tolerance to salt stress.

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Changes in activity profile of superoxide dismutase in barley cultivars seedling under salt stress

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