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Changes in residual concentration of PAHs in planted and bioaugmented crude oil polluted tropical soils

Ifechukwu Enyinnaya Adieze, Justina Chibuogwu Orji, Rose Nkechinyere Nwabueze, Geoffrey Okike C. Onyeze

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J. Bio. Env. Sci.6(6), 307-316, June 2015


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Effects of bioaugmentation of vegetated crude oil polluted soils on the residual concentration of PAHs were examined in microcosms in a green house study. Weathered crude oil polluted soils (2% w/w) in 4 sets of pots were respectively planted and bioaugmented with hydrocarbon utilizing species (Micrococcus sp. RZI, Pseudomonas sp. RZIII, Bacillus sp. RZIII and Bacillus sp. GSIII). Polluted vegetated and polluted non-vegetated control soil samples were also set up. At intervals between setup and the tenth week of incubation, soil samples from three replicates of each treatment chosen randomly were analysed for soils’ residual PAHs concentrations. The results obtained showed that 10 weeks after planting (WAP) residual PAHs concentrations in polluted soils were 34.3 mg/kg; 31.6 mg/kg; 26.1 mg/kg and 25.9 mg/kg respectively for vegetated control soil, vegetated soil bioaugmented with Micrococcus sp.RZI, vegetated soil bioaugmented with Bacillus sp. RZIII and vegetated soil bioaugmented with Pseudomonas sp. RZIII. Compared to concentrations in polluted non-vegetated samples, there was 78.8%, 76.6%, 80.8% reductions in pyrene, benzo (b) fluoranthene, indeno (1,2,3-C-D) pyrene and 1,2,5,6-dibenzanthracene respectively in polluted vegetated samples bioaugmented with Pseudomonas sp. RZIII. These show that bioaugmentation of vegetated polluted soils with hydrocarbon utilizing species can enhance PAHs removal from weathered petroleum polluted soils. This can be used to improve on the gains of phytoremediation particularly with respect to carcinogenic PAHs.


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Changes in residual concentration of PAHs in planted and bioaugmented crude oil polluted tropical soils

Adieze IE. 2012. Effect of bioaugumentation on soil microbial populations and residual crude oil concentration of a polluted tropical soil. Journal of Nigerian Environmental Society. 7.

Adieze IE, Nwabueze RN, Onyeze GOC. 2003. Effect of poultry manure on the microbial utilization of hydrocarbons in oil–polluted soil. Nigerian Journal of Microbiology 17, 12 – 16.

Adieze IE, Orji JC, Nwabueze RN, Onyeze GOC. 2012. Hydrocarbon stress response of four tropical plants in weathered crude oil contaminated soil in microcosms, International Journal of Environmental Studies. 69, 490-500. http://dx.doi.org/10.1080/00207233.2012.665785.

American Public Health Association. 1985. Standard methods for water and waste water analyses, 13th ed., Washington D.C.

Banks MK, Govindaraju RS, Schwab AP, Kulakow P. 2000. Part I; Field demonstration. In: Fiorenza S, Oubre CL, Ward CH. (eds.) Phytoremediation of hydrocarbon-contaminated soil. Lewis Publishers, Boca Raton, Fl. 3-88.

Binet P, Portal JM, Leyval C. 2000. Dissipation of 3±6-ring polycyclic aromatic hydrocarbons in the rhizosphere of ryegrass. Soil Biology and Biochemistry 32, 2011 -2017.

Cunningham SD, Anderson TA, Schwab AP, Hsu FC. 1996. Phytoremediation of soils contaminated with organic pollutants. Advances in Agronomy 56, 55 – 114.

Diab EA. 2008. Phytoremediation of Polycyclic Aromatic Hydrocarbons (PAHs) in a Polluted Desert Soil, with Special Reference to the Biodegradation of the Carcinogenic PAHs, Australian Journal of Basic and Applied Sciences 2, 757-762.

Epuri V, Sorensen DL. 1997. Benzo(a)pyrene and hexachlorobiphenyl contaminated soil: phytoremediation potential. In: Kruger EL, Anderson TA, Coats JR, Ed. Phytoremediation of Soil and Water Contaminants. American Chemical Society: Washington, D.C. ACS Symposium Series 664, pg. 200 – 222.

Ferro AM, Rock SA, Kennedy J, Herrick JJ, Turner DL. 1999. Phytoremediation of soils contaminated with wood preserva- tives: Greenhouse and field evaluations. International Journal of Phytoremediation 1, 289–306.

Frick CM, Farrel RE, Germida JJ. 1999. Assessment of Phytoremediation as an In-situ Technique for Cleaning Oil-Contaminated Sites, Petroleum Technology Alliance of Canada, Calgary.

Huang XD, El-Alawi Y, Penrose DM, Glick BR. Greenberg BM. 2004. A multi-process phytoremediation system for removal of polycyclic aromatic hydrocarbons from contaminated soils. Environmental Pollution 130, 465-476.

Kalf DF, Commentuijn T, Vande Plassche EJ. 1997. Environmental quality objectives for 10 polycyclic aromatic hydrocarbons (PAHs), Ecotoxicology and Environmental Saftey 36, 89-97.

Kanaly R, Bartha R, Fogel S, Findlay M. 1997.Biodegradation of [14C]benzo[a]pyrene added in crude oil to uncontaminated soil. Applied and Environmental Microbiology 63, 4511-4515.

Liste HH, Alexander M. 2000. Plant-promoted pyrene degradation in soil. Chemosphere 40, 7-10.

Mishra S, Jyot J, Kuhad RC, Lal B. 2001. In situ bioremediation potential of an oily sludge-degrading bacterial consortium. Current Microbiology 43, 328-335.

Nasseri S, Kalantary R, Nourieh N, Naddafi K, Mahvi A, Baradaran N. 2010. Influence of bioaugmentation in biodegradation of PAHs-contaminated soil in bio-slurry phase reactor. Iranian Journal of Environmental Health Science and Engineering 7, 199–208.

Nweke CO, Mgbachi LC, Nwanganga C, Nwanyanwu CE. 1996. Heavy metal tolerance among hydrocarbon utilizing bacteria isolated from oil-contaminated soil. Nigerian Journal of Microbiology 20, 1057 – 1065.

Odokuma LO, Dickson AA. 2003. Bioremediation of a crude oil polluted Tropical rain forest soil. Global Journal of Environmtal Sciences 2, 29-40.

Orji  JC,  Adieze  IE,  Nwabueze  RN,  Onyeze GOC. 2015. Effect of bioaugmentation of crude oil polluted  tropical  soils  on  the  growth  of  Panicum maximum. Journal of Biodiversity and Environmental Sciences 6, 227-235. http://www.innspub.net

Reilley KA, Banks MK, Schwab AP. 1996. Organic chemicals in the environment: dissipation of polycyclic aromatic hydrocarbons in the rhizosphere. Journal of Environmental Quality 25, 212-219.

Sheng-wang PAN, Shi-qiang WEI, Xin Y, Sheng-xian CAO. 2008. The removal and remediation of phenanthrene and pyrene in soil by mixed cropping of alfalfa and rape. Agricultural Sciences in China 7, 1355–1364. http://dx.doi.org/10.1016/S1671-2927(08)60185-6.

Siciliano SD, Germida JJ. 1997. Bacterial inoculants of forage grasses that enhance degradation of 2-chlorobenzoic acid in soil. Environmental Toxicology and Chemistry 16, 1098-1104.

Yu XZ, Wu SC, Wu FY, Wong MH. 2010. Enhanced dissipation of PAHs from using mycorrhizal ryegrass and PAH-degrading bacteria. Journal of Hazardous Materials 186, 1206-1217.


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