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Research Paper | December 1, 2016

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Characterization and identification of wheat (Triticum aestivum L.) rhizospheric bacteria by using 16s rRNA gene sequencing

Rizwan Ali Sheirdil, Rifat Hayat, Xiao-Xia Zhang, Nadeem Akhtar Abbasi, Safdar Ali, Muhammad Azeem

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J. Bio. Env. Sci.9(6), 31-38, December 2016

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Abstract

Bacterial strains were isolated from wheat rhizospheric soil with the aim to explore potential strains for the biofertilizer technology suitable to the local climate and conditions. All the isolates were characterized for their plant growth promoting (PGP) activities such as IAA production, P-solublization, nifH gene presence, catalase and oxidase production. The promising and potential strains based on their PGP activities were tested in two experiments of wheat. In the first growth chamber experiment, ten strains were inoculated with the seeds and sown in small pots filled with the soil. All the strains showed positive results in yield attributes of the plant like shoot length, root length, fresh and dry weight. Further, six strains which performed best in the growth chamber experiment were tested in a pot experiment under controlled conditions and the results showed that each strain significantly increased like shoot length (7.6-25%), root length (19.7-34.1%), number of tillers (33.3-100%), fresh weight (17.8-31.3%), dry weight (19.3-48.6%), spike length (4.3-28.2%) and grain yield (7.6-41.5%) of wheat plants over control. The strains used in the pot experiment were identified by standard procedure of 16s rRNA gene sequencing which showed that two of strains were from genus Bacillus, two from Pseudomonas, one from Acinetobacter and one from Jeotgalicoccus.

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Characterization and identification of wheat (Triticum aestivum L.) rhizospheric bacteria by using 16s rRNA gene sequencing

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