Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | November 1, 2019

| Download

Characterization, antagonistic effect, resistant pattern and adaptation capability of halophilic bacteria isolated from Inani Beach, Cox’s Bazar, Bangladesh

Sheikh Farah Diba, Md. Nazmul Haque, Md. Akhtar-E-Ekram, Md. Abu Saleh, Shahriar Zaman, Md. Salah Uddin

Key Words:

Int. J. Biosci.15(5), 615-625, November 2019

DOI: http://dx.doi.org/10.12692/ijb/15.5.615-625


IJB 2019 [Generate Certificate]


The ocean, with its rich salt tolerant microbial biodiversity, continues to serve as a source of potential salt tolerant gene. In this study, bacteria are isolated from marine water collected from the Inani Beach, Cox’s Bazar, Bangladesh. Bacterial strains were inoculated in standard Luria-Bertani (LB) medium with 2% of NaCl. Two types of bacterial strain A and B were isolated according to the morphology and nature of colonies. Morphological and biochemical properties of bacteria indicated that strain A was cream colored, gram negative, round shaped, non-motile catalase positive and showed negative result in methyl red test. Strain B was creamy white colored, gram positive, rod shaped, non-motile and showed positive results in both methyl red and catalase test. The adaptability of bacterial samples were examined on different laboratory conditions and also observed their potential as a sources of antimicrobial agents. The optimum physiological conditions for both isolates were at pH 8, temperature 35°C and 2gm/l of NaCl. Both of the strains were highly resistant to Tetracycline, Cefuroxime, Erythromycin, Doxycycline, and Carbenicillin. Bacterial strain A and B both have the ability to utilize glucose, sugar, yeast extract and glycerol as their sole source of carbon. Among these yeast extract was proved as the best source of carbon. The present investigation also revealed that the isolated bacteria B have antagonistic effects on the human pathogenic bacteria Staphylococcus aureus and Escherichia coli. Both of the isolates A and B were cultured in LB media without addition of NaCl. The growth rates of isolate A was significantly higher than isolate B in the medium and growth rates were increasing in every subcultures. Therefore, the study concluded that the strain has strong adaptation capability in normal environmental condition.


Copyright © 2019
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Characterization, antagonistic effect, resistant pattern and adaptation capability of halophilic bacteria isolated from Inani Beach, Cox’s Bazar, Bangladesh

Alexander DE. 1999. Encyclopedia of environmental science. Springer, New York: 0-412-74050-8.

Arvanitidou M, Katsosyannopoulos V, Tsakris A. 2001. Antibiotic resistance patterns of enterococci isolated from coastal bathing waters. Journal of Medical Microbiology 50, 1001–1005. https://doi.org/10.1099/0022-1317-50-11-10.01

Atta HM, Ahmad MS. 2009. Antimycin-A antibiotic biosynthesis produced by Streptomyces sp. AZ-AR-262: Taxonomy, fermentation, purification and biological activities. Australian Journal of Basic and Applied Sciences 3, 126-135.

Bedford RH. 1932. Marine bacteria of the northern Pacific Ocean the temperature range of growth. Contributions to Canadian Biology and Fisheries 7, 431-438.

Chowdhury JB, Jain S, Jain RK. 1993. Biotechnological approaches for developing salt tolerant field crops. Journal of Plant Biochemistry and Biotechnology 2, 1-7. https://doi.org/10.1139/f32-034

Dalmaso GZL, Ferreira D, Vermelho AB. 2015.  Marine extremophiles: a source of hydrolases for biotechnological applications. Marine Drugs 13, 1925-1965. https://doi.org/10.3390/md13041925

De Oliveira AJFC, De Franca PTR, Pinto AB. 2010. Antimicrobial resistance of heterotrophic marine bacteria isolated from seawater and sands of recreational beaches with different organic pollution levels in southeastern Brazil: evidences of resistance dissemination. Environmental Monitoring and Assessment 169, 375-384.

Donia M, Humann MT. 2003. Marine natural products and their potential applications as anti-infective agents. The Lancet Infectious disease 3, 338-348. https://doi.org/10.1016/s1473-3099(03)00655-8

Faulkner DJ. 2001. Marine natural products. Natural product reports 18, 1-49 https://doi.org/10.1039/B006897G

Gontang EA, Fenical W, Jensen PA. 2007. Phylogenetic diversity of gram-positive bacteria cultured from marine sediments. Applied and Environmental Microbiology 73, 3272–3282. https://doi.org/10.1128/AEM.02811-06

Hase CC, Fedorova ND, Galperin MY, Dibrov PA. 2001. Sodium ion cycle in bacterial pathogens: evidence from cross-genome comparisons. Microbiology and Molecular Biology Reviews 65, 353– 370. https://doi.org/10.1128/MMBR.65.3.353-370.2001

Kim TK, Hewavitharana AK, Shaw PN, Fuerst JA. 2006. Discovery of a new source of rifamycin antibiotics in marine sponge actinobacteria by phylogenetic prediction. Applied and Environmental Microbiology 72, 2118–2125. https://doi.org/10.1128/AEM.72.3.2118-2125.2006

Lauro FM, McDougald D, Thomas T, Williams TJ, Egan S, Rice S, DeMaere MZ, Ting L, Ertan H, Johnson J, Ferriera S, Lapidus A, Anderson I, Kyrpides N, Munk AC, Detter C, Hang CS, Brown MV, Robb FT, Kjelleberga S,  Cavicchiol R. 2009. The genomic basis of trophic strategy in marine bacteria.  Proceedings of the National Academy of Sciences of the United States of America 106, 15527 –15533. https://doi.org/10.1073/pnas.09035.07106

MacLeod RA, Onofrey E. 1957. Nutrition and metabolism of marine bacteria III. The relation of sodium and potassium to growth. Journal of Cellular Physiology 50, 389 – 401. https://doi.org/10.1002/jcp.1030500305

Nagasathya A, Thajuddin N. 2008. Cyanobacterial Diversity in the Hypersaline Environment of the Saltpans of Southeastern Coast of India. Asian Journal of Plant Sciences 7, 473-478. https://doi.org/10.3923/ajps.2008.473.478\

Narayana JK, Prabhakar P, Vijayalakshmi M, Venkateswarlu Y, Krishna PS. 2007. Biological activity of phenylpropionic acid isolated from a terrestrial Streptomycetes. Polish Journal of Microbiology 56, 191-197.

Newman DJ, Cragg GM. 2004. Marine natural products and related compounds in clinical and advanced preclinical trials. Journal of Natural Products 67, 1216–1238. https://doi.org/10.1021/np040031y

Pabba SK, Samatha B, Prasad MR, Himabindu S. 2011. Isolation and screening of marine bacteria for antimicrobial activity along Vishakapatanam Coast. Journal of Microbiology and Biotechnology Research 1, 86 – 88.

Pratt D, Happold FC. 1960. Requirements for indole production by cells and extracts of a marine bacterium. Journal of Bacteriology 80, 232 – 236.

Reddy NG, Ramakrishna DPN, Gopal SVR. 2011. A morphological, physiological and biochemical studies of marine Streptomyces rochei (MTCC 10109) showing antagonistic activity against selective human pathogenic microorganisms. Asian Journal of Biological Science 4, 1-14. https://doi.org/10.3923/ajbs.2011.1.14

Rhodes ME, Payne WJ. 1962. Further observations on effects of cations on enzyme induction in marine bacteria. Antonie van Leeuwenhoek. Journal of Microbiology 28, 302-314.

Takizawa M, Colwell RR, Hill RT, 1993. Isolation and Diversity of Actinomycetes in the Chesapeake Bay. Applied and Environmental Microbiology 59, 997-100.

Ventosa A, Nieto JJ, Oren A. 1998. Biology of moderately halophilic aerobic bacteria. Microbiology and Molecular Biology Reviews 62, 504 – 544.

Ventosa A. 2004. Halophilic Microorganisms. Microbial Molecular and Physiological Diversity in Hypersaline Environments. Heidelberg: Springer, p 49-61.

Zobell CE, Morita RY. 1957.  Barophilic bacteria in some deep sea sediments. Journal of Bacteriology 73, 563–568.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background