Characterization of Chitosan Extracted from Exoskeletons of Pomacea spp.

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Research Paper 28/07/2022
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Characterization of Chitosan Extracted from Exoskeletons of Pomacea spp.

Unnis P. Aguilor, Renjith Raju, Jitty Raju, Julius T. Capili
Int. J. Biosci. 21(1), 184-191, July 2022.
Copyright Statement: Copyright 2022; The Author(s).
License: CC BY-NC 4.0

Abstract

This study focused mainly on the characterization of the chitosan sample extracted from exoskeletons of Pomacea spp., a freshwater mollusk. FTIR was used to characterize the chitosan sample and was compared to the commercial chitosan, which has a DD value of 95%. The FTIR spectra formed characteristic bands in the frequency range between 4000 and 400 cm−1. The FTIR of chitosan samples isolated from shells of Pomacea spp. and the commercial chitosan yielded spectra with functional groups where the hydroxyl (OH) group is at 3650 to 3400 cm-1; carbonyl (C=O) group vibration is at 1730 cm-1 and amide I group is at 1650 to 1550 cm-1. Moreover, the locally extracted chitosan samples from Pomacea spp. shells showed FTIR spectra that are nearly comparable with the commercial chitosan and those locally extracted chitosan samples used in the previous studies. Analysis by IR estimated the DD as 60.47% for this chitosan. This DD value was 56% to 99% when a sample can be considered chitosan.

Anwar M, Anggraeni AS, Amin MHA. 2017. Comparison of Green Method for Chitin Deacetylation. International Conference on Chemistry, Chemical Process and Engineering (IC3PE) 2017. AIP Conference Proceedings 1823, 020071-1–020071-6. http://dx.doi.org/10.1063/1.4978144

Bariuan VJT, Mabborang CT, Macanas SMG, Santiago KCO, Salomon JB, Chua JMT, Jr, RBR. 2020. Mollugo oppositifolia leaf tea formulation and its effect against alloxan-induced hyperglycemic male sprague dawley rats. International Journal of Biosciences 16(4), 71-78. http://dx.doi.org/10.12692/ijb/16.4.71-78.

Biskup RC, Jarosińska D, Rokita B, Ulański P, Rosiak JM. 2012. Determination of degree of deacetylation of Chitosan – Comparison of methods. https://www.researchgate.net/publication/288104933.

Boukhlifi F. 2020. Quantitative Analysis by IR: Determination of Chitin/Chitosan DD.

Chabbra P. 2001. Antimicrobial and antioxidant properties of chitosan.

Domszy JG, Roberts GAF. 1985. Evaluation of infrared spectroscopic techniques for analyzing chitosan. Die Makromolekulare Chemie 186, 1671-1677.

Kaewboonruang S. 2016. Comparative studies on the extraction of chitin chitosan from golden apple snail shells at the control field.

Kalut SA. 2008. Enhancement of degree of deacetylation of chitin in chitosan production.

Khan TA, Peh KK Ch’ng HS. 2002. Reporting degree of deacetylation values of Chitosan: the influence of analytical methods. Journal of Pharmaceutical Sciences 5(3), 205-212, 2002.

Loutfy SM, El-Din HAM, Elberry MH Allam, NG, Hasanin MTM, Abdellah AM. 2016. Synthesis, characterization and cytotoxic evaluation of chitosan nanoparticles: in vitro liver cancer model. Advanced Natural Science: Nanoscience Nanotechnology 7, 035008 (9p). http://dx.doi.org/10.1088/2043-6262/7/3/035008.

Majekodunmi SO, Olorunsola EO. Uzoaganobi CC. 2017. Comparative physicochemical characterization of chitosan from shells of two bivalved mollusks from two different continents. American Journal of Polymer Science 7, 15−22.

Muñoz G, Valencia C, Valderruten N, Ruiz-Durantez E, Zuluaga F. 2015. Extraction of Chitosan from Aspergillus niger mycelium and synthesis of hydrogels for controlled release of betahistine. Reactive and Functional Polymers 91−92, 1−10.

Murugan K, Anitha K, Dinesh D, Suresh U, Ra jaganesh R, Chandramohan B, Subramania m J, Paulpandi M, Vadivalagan C, Amuthavalli P, Wang L, Hwang JS, Wei H, Alsalhi MS, Devan esan S, Kumar S, Pugazhendy K, Higuchi A, Nicoletti M, Benelli G. 2017. Fabrication of nanomos quitocides using chitosan from crab shells: Impact on nontarget organisms in the aquatic environment.

Muslim T, Rhman MH, Begmun A, Rhman A. 2012. Chitosan and Carboxymethyl Chitosan from Fish Scales of Labeorohitin.

Nandiyanto ABD, Risti Ragadhita RO. 2019. How to read and interpret FTIR Spectroscopic of Organic Material. Indonesian Journal of Science and Technology. http://ejournal.upi.edu/index.php/ijost/

Oyekunle DT, Omoleye JA. 2019. New process for synthesizing chitosan from snail shells. IOP Conf. Series: Journal of Physics: Conference Series 1299 (2019) 012089. http://dx.doi.org/10.1088/17426596/1299/1/012089

Paul S, Jayan A, Sasikumar CS, Cherian SM. 2014. Extraction and purification of Chitosan from chitin isolated from sea prawn (Fenneropenaeus Indicus). ISSN – 0974-244.

Ssekatawa K, Byarugaba DK, Wampande EM, Moja TN, Nxumalo E, Maaza M, Sackey J, Ejobi F, Kirabira JB. 2021. Isolation and characterization of Chitosan from Ugandan edible mushrooms, Nile perch scales and banana weevils for biomedical applications. https://doi.org/10.1038/s41598-021-81880-7.

Synowiecki J, Al-khatteb NAA. 2003. Production, properties and some new applications of chitin and its derivatives. Critical Reviews in Food Science and Nutrition 43, 144-171.

Thillai N.S., Kalyanasundaram, N. and Ravi, S. 2017. Extraction and Characterization of Chitin and Chitosan from Achatinodes. Thillai Natarajan, Natural Products Chemistry & Research 5(6). http://dx.doi.org/10.4172/2329-6836.1000281.

Varma R, Vasuden S. 2020. Extraction, Characterization, and Antimicrobial Activity of Chitosan from Horse Mussel Modiolus modiolus. https://dx.doi.org/10.1021/acsomega.0c01903.

Vilar Junior JC, Ribeaux DR, Alves da Silva CA, Campos-Takaki D, Maria G. 2016. Physicochemical and antibacterial properties of chitosan extracted from waste shrimp shells. International Journal of Microbiology 2016, 5127515. https://doi.org/10.1155/2016/5127515.

Younes I, Rinaudo M. 2015. Chitin and Chitosan Preparation from Marine Sources. Structure, Properties and Applications. Marine Drugs 13(3), 1133-1174. http://dx.doi.org/10.3390/md13031133.

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