Characterization of Fusarium spp associated with mango malformation diseases in the South of Senegal

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Research Paper 05/11/2022
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Characterization of Fusarium spp associated with mango malformation diseases in the South of Senegal

Coumba Fall, Nalla Mbaye, Papa Madiallacke Diedhiou, Fatou Ndoye, Cheikh Dieye
Int. J. Agron. Agri. Res.21( 5), 1-10, November 2022.
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Abstract

Mango is a fruit with huge economic importance in Senegal, as in many tropical and subtropical countries in the world. Its production and trade increased tremendously since exportation started in 1997. However, mango diseases like floral and vegetative malformation is one of the most important constraints causing serious economic losses. Several species of the Fusarium genus have been associated to this disease. In Senegal, only one study has been carried out on mango malformation diseases in the south and Fusarium tupiense was identified as the causal agent. The present study was undertaken to explore the diversity of strains/species of the causal agents of mango malformation diseases in Senegal. Strains of Fusarium spp isolated from malformed mango flowers of different trees, collected in different locations in southern Senegal were characterized on the basis morphological criteria and using molecular biology. The ITS region from the ribosomal DNA was targeted for the molecular biology analysis. The morphological characterization brought out a diversity of Fusarium structures associated with mango malformation suggesting diverse species. The molecular characterization allowed a better refinement, confirming the diversity of species without leading to a definitive specific identification. Therefore, a molecular characterization with specific primers should be considered.

VIEWS 101

Aamir S, Sutar S, Singh SK, Baghela A. 2015. A rapid and efficient method of fungal genomic DNA extraction, suitable for PCR based molecular methods. Plant Pathology & Quarantine 5(2), 74-81.

Ansari MW, Rani V, Shukla A, Bains G, Pant RC, Tuteja C. 2015. Mango (Mangifera indica L.) malformation: A malady of stress ethylene origin. Physiol Mol Biol Plants 21(1), 1-8

Booth C. 1971. The genus Fusarium. Commonwealth Mycological Institute,

Britz H, Steenkamp ET, Coutinho TA, Wingfield BD, Marasas WFO, Wingfield FJ. 2002. Two new species of Fusarium section Liseola associated with mango malformation. Mycologia 94(4), 722-730.

Cadre Intégré Renforcé. 2017 : Bilan de la campagne 2017 d’exportation de mangue, consulté le 28/10/2018 de http://atlc.sn/bilan-de-la-campagne-2017-dexportation-de-mangue/

Diédhiou PM, Diop SAG, Mbaye N, Diédhiou I, Diallo Y, Djiba S, Faye R, Samb PI. 2014. Mango rotting in southern Senegal, a big phytosanitary challenge. International Journal of Biosciences 5(5), 183-188.

Dieye C, Houmairi H, Diedhiou PM, Gaboune F, Mbaye N, Diallo Y, Bencharki B. 2021. “Characterization of Fusarium species associated with mango malformation disease in southern Senegal.” International Journal of Advanced Research (IJAR) 9(3), 322-331.

Felsenstein J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783-791.

Freeman S, Maimon M, Pinkas Y. 1999. Use of GUS Transformants of Fusarium subglutinans for Determining Etiology of Mango Malformation Disease. Phytopathology 89, 456-461.

Freeman S, Shtienberg D, Maymon M, Levin AG, Ploetz RC. 2014. New Insights into Mango Malformation Disease Epidemiology Lead to a New Integrated Management Strategy for Subtropical Environments. The American Phytopathological Society 98, 1456-1466.

Iqbal Z, Dasti AA, Saleem A. 2005. Combating mango malformation through an integrated management strategy. International Conference on Mango and Date palm: Culture and Export, 20-23 June 2005, University of Agriculture, Faisalabad.

Kumar P, Kamle M, Misra AK, O’Donovan A, Pagano M, Modi DR. 2016. Identification and characterization of Fusarium mangiferae as pathogen of mango malformation in India. Brazilian Archives of Biology and Technology 59, 1-9.

Le citoyen. 2017. Les potentialités de la filière mangue du Sénégal, consulté le 28 octobre 2018 de https://espritcitoyen.com/les-potentialites-de-la-filiere-mangue-du-senegal_p_402.html

Leslie JF, Summerell BA. 2006. The Fusarium laboratory manual. Blackwell Publishing: 2121 State Avenue, Ames, Iowa50014, USA. 399p.

Lima CS, Pfenning LH, Costa SS, Abreu LM, Leslie JF. 2012. Fusarium tupiense sp. nov., a member of the Gibberella fujikuroi complex that causes mango malformation in Brazil. Mycologia 104, 1408-1419.

Marasas WFO, Ploetz RC, Wingfield MJ, Wingfield BD, Steenkamp ET. 2006. Mango Malformation Disease and the Associated Fusarium species. Phytopathology 96, 667-672.

Moine L. 2013. Identification et détection d’une nouvelle espèce de Fusarium pathogène sur la tomate de serre en Amérique du Nord. Mémoire de Master, Université Laval, Canada.

Otero-Colina G, Rodríguez-Alvarado G, Fernández-Pavía S, Maymon M, Ploetz RC, Takayuki A, O’Donnell K, Freeman S. 2010. Identification and Characterization of a Novel Etiological Agent of Mango Malformation Disease in Mexico, Fusarium mexicanum. Phytopathology 100, 1176-1184.

Planétoscope. 2018 : La production mondiale de mangues, consulté le 28 octobre 2018 de https://www.planetoscope.com/fruits-legumes/1481-production-mondiale-de-mangues.html

Rey JY. 2011. La production fruitière sénégalaise. Thiès, 08 décembre 2011. 5p

Senghor AL, Sharma K, Kumar PL, Bandyopadhyay R. 2012. First Report of Mango Malformation Disease Caused by Fusarium tupiense in Senegal. The American Phytopathological Society 96, 1582.

Sylla F. 2010, A mango disease new to Senegal and discovered last year threatens disaster for mango production. Global Agricultural Information Network, Le 19/08.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10), 2731-2739.

Youssef SA, Maymon M, Zveibil A, Klein-Gueta D, Sztejnberg A, Shalaby AA, Freeman S. 2006. Epidemiological aspects of mango malformation disease caused by Fusarium mangiferae and source of infection in seedlings cultivated in orchards in Egypt. Plant Pathology 56, 257-263.