Characterization of genetic variation between local and exotic Iranian strawberry (Fragaria×ananassa Duch.) cultivars using amplified fragment length polymorphism (AFLP) markers

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Research Paper 01/12/2012
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Characterization of genetic variation between local and exotic Iranian strawberry (Fragaria×ananassa Duch.) cultivars using amplified fragment length polymorphism (AFLP) markers

Yahya Manbari, Ezzat Karami, Ali Etminan, Reza Talebi
Int. J. Biosci.2( 12), 159-167, December 2012.
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Abstract

The goal of this study was to determine the level of genetic diversity and relatedness among 17 local strawberry cultivars collected from ‘Kurdistan’ province (north-west of Iran) with 13 exotic cultivars, using ten AFLP markers. The 10 AFLP primer pairs produced 228 polymorphic bands. The number of polymorphic bands per primers ranged from 11 to 33, whereas the maximum was observed in E36/M35 primer pairs. The genetic diversity (GD) and markers PIC values ranged from 0.28 to 0.77 and 0.33 to 0.48, with an average 0.54 and 0.41, respectively. Cluster analysis grouped cultivars in four distinct clusters and all local and exotic cultivars separated in referred clusters. We obtained narrow range of diversity in the group of exotic cultivars (from 0.27 to 0.44) while higher diversity values (from 0.33 to 0.77) were observed in local cultivars. The results clearly demonstrate that AFLP markers can be used in a genetic diversity study as well as in genotypic identification of strawberries.

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